Lentivirus-mediated RNA interference in mammalian neurons

Methods in Molecular Biology

Volumn 442, Issue , 2008, Pages 95-112

  Harper, Scott Q ^a   Gonzalez Alegre, Pedro ^b  

^a OHIO STATE UNIVERSITY   (United States)

^b UNIVERSITY OF IOWA   (United States)

Author keywords

FIV (feline immunodeficiency virus); Lentivirus; PNC (primary neuronal cultures); RNAi; ShRNA; ; Silencing; TorsinA

Indexed keywords

FELINE IMMUNODEFICIENCY VIRUS; LENTIVIRUS; MAMMALIA;

EID: 84934434586     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-191-8_8     Document Type: Article

References (14)

1. Ozelius, L. J., Hewett, J. W., Page, C. E., et al. (1997). The early-onset torsion dystonia gene (DYT1) encodes an ATP-binding protein. Nat. Genet. 17, 40-48.
2. Gonzalez-Alegre, P., and Paulson, H. L. (2004). Aberrant cellular behavior of mutant torsinA implicates nuclear envelope dysfunction in DYT1 dystonia. J. Neurosci. 24, 2593-2601.
3. Goodchild, R. E., and Dauer, W. T. (2004). Mislocalization to the nuclear envelope: An effect of the dystonia-causing torsinA mutation. Proc. Natl. Acad. Sci. USA. 101, 847-852.
4. Naismith, T. V., Heuser, J. E., Breakefield, X. O., and Hanson, P. I. (2004). TorsinA in the nuclear envelope. Proc. Natl. Acad. Sci. USA 101, 7612-7617.
5. Goodchild, R. E., Kim, C. E., and Dauer, W. T. (2005). Loss of the dystoniaassociated protein torsinA selectively disrupts the neuronal nuclear envelope. Neuron 48, 923-932.
6. Walker, R. H., and Shashidharan, P. (2003). Developments in the molecular biology of DYT1 dystonia. Mov. Disord. 18, 1102-1107.
7. Gonzalez-Alegre, P., Miller, V. M., Davidson, B. L., and Paulson, H. L. (2003). Toward tiierapy for DYT1 dystonia: Allele-specific silencing of mutant torsinA. Ann. Neurol. 53, 781-787.
8. Gonzalez-Alegre, P., Bode, N., Davidson, B. L., and Paulson, H. L. (2005). Silencing primary dystonia: Lentiviral-mediated RNA interference therapy for DYT1 dystonia. J. Neurosci. 25, 10502-10509.
9. Meberg, P. J., and Miller, M. W. (2003). Culturing hippocampal and cortical neurons. Meth. Cell Biol. 71, 111-127.
10. Harper, S. Q., Staber, P. D., Beck, C. R., et al. (2006). Optimization of feline immunodeficiency virus vectors for RNA interference. J. Virol. 80, 9371-9380.
11. Rubinson, D. A., Dillon, C. P., Kwiatkowski, A. V., et al. (2003). A lentivirusbased system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat. Genet. 33, 401-406.
12. Davidson, B. L., and Harper, S. Q. (2005). Viral delivery of recombinant short hairpin RNAs. Meth. Enzymol. 392, 145-173.
13. Johnston, J. C., Gasmi, M., Lim, L. E., et al. (1999). Minimum requirements for efficient transduction of dividing and nondividing cells by feline immunodeficiency virus vectors. J. Virol. 73, 4991-5000.
14. Hewett, J., Ziefer, P., Bergeron, D., et al. (2003). TorsinA in PC12 cells: Localization in the endoplasmic reticulum and response to stress. J. Neurosci. Res. 72, 158-168.