Crystal structure of β-galactosidase from Bacillus circulans ATCC 31382 (BgaD) and the construction of the thermophilic mutants

FEBS Journal

Volumn 282, Issue 13, 2015, Pages 2540-2552

  Ishikawa, Kazuhiko ^a   Kataoka, Misumi ^a   Yanamoto, Toshiaki ^a   Nakabayashi, Makoto ^a   Watanabe, Masahiro ^a   Ishihara, Satoru ^b   Yamaguchi, Shotaro ^b  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b Amano Enzyme Inc   (Japan)

Author keywords

crystal structure; galacto oligosaccharide; prebiotics; protein engineering; thermophilic

Indexed keywords

BACILLUS CIRCULANS BETA GALACTOSIDE; BETA GALACTOSIDE; GLYCOSYLTRANSFERASE; UNCLASSIFIED DRUG; BETA GALACTOSIDASE;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS CIRCULANS; BETA SHEET; CARBOXY TERMINAL SEQUENCE; CIRCULAR DICHROISM; CONTROLLED STUDY; CRYSTAL STRUCTURE; CRYSTALLIZATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME SPECIFICITY; GLYCOSYLATION; IMMUNOGLOBULIN LIKE DOMAIN; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STRUCTURE ANALYSIS; THERMOPHILIC BACTERIUM; THERMOSTABILITY; X RAY ANALYSIS; BACILLUS; CHEMISTRY; ENZYMOLOGY; MUTATION; PROTEIN TERTIARY STRUCTURE;

BACILLUS CIRCULANS; BACTERIA (MICROORGANISMS);

BACILLUS; BETA-GALACTOSIDASE; CATALYTIC DOMAIN; CRYSTALLIZATION; MUTATION; PROTEIN STRUCTURE, TERTIARY;

EID: 84934298944     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/febs.13298     Document Type: Article

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