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Volumn , Issue , 2014, Pages 3678-3701

Reagents and Cell Staining for Immunophenotyping by Flow Cytometry

Author keywords

Antigen epitope; Cell separation; Cell viabiLity; Cluster of differentiation; Fixatives; Flow cytometry; Fluorochromes; Immunofluorescent staining; Immunophenotyping; Leukocytes; Monoclonal antibodies; Red blood cell lysis; Stain index; White blood cells

Indexed keywords


EID: 84932197036     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1016/B978-0-12-386456-7.07104-5     Document Type: Chapter
Times cited : (17)

References (50)
  • 1
    • 0022111678 scopus 로고
    • Ficoll-Hypaque separation of bone marrow cells
    • Abrams R.A., Buck P., Polacek L. Ficoll-Hypaque separation of bone marrow cells. Blood 1985, 66:472-473.
    • (1985) Blood , vol.66 , pp. 472-473
    • Abrams, R.A.1    Buck, P.2    Polacek, L.3
  • 2
    • 0036330398 scopus 로고    scopus 로고
    • Number of viable CD34(+) cells reinfused predicts engraftment in autologous hematopoietic stem cell transplantation
    • Allan D.S., Keeney M., Howson-Jan K., Popma J., Weir K., Bhatia M., et al. Number of viable CD34(+) cells reinfused predicts engraftment in autologous hematopoietic stem cell transplantation. Bone Marrow Transplant. 2002, 29:967-972.
    • (2002) Bone Marrow Transplant. , vol.29 , pp. 967-972
    • Allan, D.S.1    Keeney, M.2    Howson-Jan, K.3    Popma, J.4    Weir, K.5    Bhatia, M.6
  • 3
    • 0348230787 scopus 로고    scopus 로고
    • Routine flow cytometric immuno-staining of T-cell perforin is preserved using diethylene glycol for erythrocyte-lysis but lost by the use of ammonium chloride
    • Ambach A., Bonnekoh B., Gollnick H. Routine flow cytometric immuno-staining of T-cell perforin is preserved using diethylene glycol for erythrocyte-lysis but lost by the use of ammonium chloride. Exp. Dermatol. 2003, 12:825-831.
    • (2003) Exp. Dermatol. , vol.12 , pp. 825-831
    • Ambach, A.1    Bonnekoh, B.2    Gollnick, H.3
  • 5
    • 0038810211 scopus 로고    scopus 로고
    • Multicolor laser scanning confocal immunofluorescence microscopy: practical appLication and Limitations
    • Brelje T.C., Wessendorf M.W., Sorenson R.L. Multicolor laser scanning confocal immunofluorescence microscopy: practical appLication and Limitations. Methods Cell Biol. 2002, 70:165-244.
    • (2002) Methods Cell Biol. , vol.70 , pp. 165-244
    • Brelje, T.C.1    Wessendorf, M.W.2    Sorenson, R.L.3
  • 6
  • 7
    • 0021104486 scopus 로고
    • Selective loss of OKT8 lymphocytes on density gradient centrifugation separation of blood mononuclear cells
    • De PaoLi P., Villalta D., Battistin S., Gasparollo A., Santini G. Selective loss of OKT8 lymphocytes on density gradient centrifugation separation of blood mononuclear cells. J. Immunol. Methods 1983, 61:259-260.
    • (1983) J. Immunol. Methods , vol.61 , pp. 259-260
    • De PaoLi, P.1    Villalta, D.2    Battistin, S.3    Gasparollo, A.4    Santini, G.5
  • 8
    • 79955683073 scopus 로고    scopus 로고
    • Proceedings of the 9th International Workshop on Human Leukocyte Differentiation Antigens. March 2010. Barcelona, Spain
    • Engel P. Proceedings of the 9th International Workshop on Human Leukocyte Differentiation Antigens. March 2010. Barcelona, Spain. Immunol. Lett. 2011, 134:103-187.
    • (2011) Immunol. Lett. , vol.134 , pp. 103-187
    • Engel, P.1
  • 10
    • 0342314501 scopus 로고    scopus 로고
    • Loss of CD34(+) hematopoietic progenitor cells due to washing can be reduced by the use of fixative-free erythrocyte lysing reagents
    • Gratama J.W., Menendez P., Kraan J., Orfao A. Loss of CD34(+) hematopoietic progenitor cells due to washing can be reduced by the use of fixative-free erythrocyte lysing reagents. J. Immunol. Methods 2000, 239:13-23.
    • (2000) J. Immunol. Methods , vol.239 , pp. 13-23
    • Gratama, J.W.1    Menendez, P.2    Kraan, J.3    Orfao, A.4
  • 14
    • 0008066915 scopus 로고
    • The role of carbonic anhydrase in certain ionic exchanges involving the erythrocyte
    • Jacobs M.H., Stewart D.R. The role of carbonic anhydrase in certain ionic exchanges involving the erythrocyte. J. Gen. Physiol. 1942, 25:539-552.
    • (1942) J. Gen. Physiol. , vol.25 , pp. 539-552
    • Jacobs, M.H.1    Stewart, D.R.2
  • 15
    • 15244353941 scopus 로고    scopus 로고
    • Phosphorothyoate oLigodeoxynucleotides block nonspecific binding of Cy5 conjugates to monocytes
    • Jahrsdorfer B., Blackwell S.E., Weiner G.J. Phosphorothyoate oLigodeoxynucleotides block nonspecific binding of Cy5 conjugates to monocytes. J. Immunol. Methods 2005, 297:259-263.
    • (2005) J. Immunol. Methods , vol.297 , pp. 259-263
    • Jahrsdorfer, B.1    Blackwell, S.E.2    Weiner, G.J.3
  • 18
    • 0016756272 scopus 로고
    • Continuous cultures of fused cells secreting antibody of predefined specificity
    • Kohler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 1975, 256:495-497.
    • (1975) Nature , vol.256 , pp. 495-497
    • Kohler, G.1    Milstein, C.2
  • 19
    • 23444446774 scopus 로고    scopus 로고
    • Coordinate analysis of murine immune cell surface markers and intracellular phosphoproteins by flow cytometry
    • Krutzik P.O., Clutter M.R., Nolan G.P. Coordinate analysis of murine immune cell surface markers and intracellular phosphoproteins by flow cytometry. J. Immunol. 2005, 175:2357-2365.
    • (2005) J. Immunol. , vol.175 , pp. 2357-2365
    • Krutzik, P.O.1    Clutter, M.R.2    Nolan, G.P.3
  • 20
    • 38149077099 scopus 로고    scopus 로고
    • Post-thaw viable CD34(+) cell count is a valuable predictor of haematopoietic stem cell engraftment in autologous peripheral blood stem cell transplantation
    • Lee S., Kim S., Kim H., Baek E.J., Jin H., Kim J., et al. Post-thaw viable CD34(+) cell count is a valuable predictor of haematopoietic stem cell engraftment in autologous peripheral blood stem cell transplantation. Vox Sang. 2008, 94:146-152.
    • (2008) Vox Sang. , vol.94 , pp. 146-152
    • Lee, S.1    Kim, S.2    Kim, H.3    Baek, E.J.4    Jin, H.5    Kim, J.6
  • 21
    • 0031003208 scopus 로고    scopus 로고
    • The Q-Prep system: effects on the apparent expression of leucocyte cell surface antigens
    • Macey M.G., McCarthy D.A., Davies C., Newland A.C. The Q-Prep system: effects on the apparent expression of leucocyte cell surface antigens. Cytometry 1997, 30:67-71.
    • (1997) Cytometry , vol.30 , pp. 67-71
    • Macey, M.G.1    McCarthy, D.A.2    Davies, C.3    Newland, A.C.4
  • 22
    • 77950513197 scopus 로고    scopus 로고
    • Selecting reagents for multicolor BD flow cytometry
    • Maecker H., Trotter J. Selecting reagents for multicolor BD flow cytometry. Postepy Biochem. 2009, 55:461-467.
    • (2009) Postepy Biochem. , vol.55 , pp. 461-467
    • Maecker, H.1    Trotter, J.2
  • 24
    • 0036766494 scopus 로고    scopus 로고
    • Xenogeneic human NK cytotoxicity against porcine endotheLial cells is perforin/granzyme B dependent and not inhibited by Bcl-2 overexpression
    • Matter-Reissmann U.B., Forte P., Schneider M.K., Filgueira L., Groscurth P., Seebach J.D. Xenogeneic human NK cytotoxicity against porcine endotheLial cells is perforin/granzyme B dependent and not inhibited by Bcl-2 overexpression. Xenotransplantation 2002, 9:325-337.
    • (2002) Xenotransplantation , vol.9 , pp. 325-337
    • Matter-Reissmann, U.B.1    Forte, P.2    Schneider, M.K.3    Filgueira, L.4    Groscurth, P.5    Seebach, J.D.6
  • 25
    • 0028144891 scopus 로고
    • Effect of fixation on quantification of the expression of leucocyte function-associated surface antigens
    • McCarthy D.A., Macey M.G., Cahill M.R., Newland A.C. Effect of fixation on quantification of the expression of leucocyte function-associated surface antigens. Cytometry 1994, 17:39-49.
    • (1994) Cytometry , vol.17 , pp. 39-49
    • McCarthy, D.A.1    Macey, M.G.2    Cahill, M.R.3    Newland, A.C.4
  • 26
    • 55549135720 scopus 로고    scopus 로고
    • Effects of dextran molecular weight on red blood cell aggregation
    • Neu B., Wenby R., Meiselman H.J. Effects of dextran molecular weight on red blood cell aggregation. Biophys. J. 2008, 95:3059-3065.
    • (2008) Biophys. J. , vol.95 , pp. 3059-3065
    • Neu, B.1    Wenby, R.2    Meiselman, H.J.3
  • 28
    • 0033560889 scopus 로고    scopus 로고
    • Isotype controls - time to let go?
    • O'Gorman M.R., Thomas J. Isotype controls - time to let go?. Cytometry 1999, 38:78-80.
    • (1999) Cytometry , vol.38 , pp. 78-80
    • O'Gorman, M.R.1    Thomas, J.2
  • 29
    • 84865592407 scopus 로고    scopus 로고
    • PE-Cy5.5 conjugates bind to the cells expressing mouse DEC205/CD205
    • Park C.G., Rodriguez A., Steinman R.M. PE-Cy5.5 conjugates bind to the cells expressing mouse DEC205/CD205. J. Immunol. Methods 2012, 384:184-190.
    • (2012) J. Immunol. Methods , vol.384 , pp. 184-190
    • Park, C.G.1    Rodriguez, A.2    Steinman, R.M.3
  • 30
    • 0004257938 scopus 로고    scopus 로고
    • Wolters Kluwer Health/Lippincott WilLiams & Wilkins, Philadelphia, PA
    • Paul W.E. Fundamental Immunology 2013, Wolters Kluwer Health/Lippincott WilLiams & Wilkins, Philadelphia, PA.
    • (2013) Fundamental Immunology
    • Paul, W.E.1
  • 32
    • 84870810887 scopus 로고    scopus 로고
    • Density gradient centrifugation compromises bone marrow mononuclear cell yield
    • Posel C., Moller K., FrohLich W., Schulz I., Boltze J., Wagner D.C. Density gradient centrifugation compromises bone marrow mononuclear cell yield. PloS One 2012, 7:e50293.
    • (2012) PloS One , vol.7
    • Posel, C.1    Moller, K.2    FrohLich, W.3    Schulz, I.4    Boltze, J.5    Wagner, D.C.6
  • 33
    • 0023239474 scopus 로고
    • Analysis of T cell subsets in normal adults. Comparison of whole blood lysis technique to Ficoll-Hypaque separation by flow cytometry
    • Renzi P., Ginns L.C. Analysis of T cell subsets in normal adults. Comparison of whole blood lysis technique to Ficoll-Hypaque separation by flow cytometry. J. Immunol. Methods 1987, 98:53-56.
    • (1987) J. Immunol. Methods , vol.98 , pp. 53-56
    • Renzi, P.1    Ginns, L.C.2
  • 34
    • 47749106439 scopus 로고    scopus 로고
    • Factors affecting the antigen-antibody reaction
    • Reverberi R., Reverberi L. Factors affecting the antigen-antibody reaction. Blood Transfus. 2007, 5:227-240.
    • (2007) Blood Transfus. , vol.5 , pp. 227-240
    • Reverberi, R.1    Reverberi, L.2
  • 35
    • 0035503984 scopus 로고    scopus 로고
    • Spectral compensation for flow cytometry: visuaLization artifacts, Limitations, and caveats
    • Roederer M. Spectral compensation for flow cytometry: visuaLization artifacts, Limitations, and caveats. Cytometry 2001, 45:194-205.
    • (2001) Cytometry , vol.45 , pp. 194-205
    • Roederer, M.1
  • 36
    • 0026556554 scopus 로고
    • Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry
    • Schmid I., Krall W.J., Uittenbogaart C.H., Braun J., Giorgi J.V. Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry 1992, 13:204-208.
    • (1992) Cytometry , vol.13 , pp. 204-208
    • Schmid, I.1    Krall, W.J.2    Uittenbogaart, C.H.3    Braun, J.4    Giorgi, J.V.5
  • 39
    • 34250755570 scopus 로고    scopus 로고
    • Changes in fluorescence intensity of selected leukocyte surface markers following fixation
    • Stewart J.C., Villasmil M.L., Frampton M.W. Changes in fluorescence intensity of selected leukocyte surface markers following fixation. Cytometry A 2007, 71:379-385.
    • (2007) Cytometry A , vol.71 , pp. 379-385
    • Stewart, J.C.1    Villasmil, M.L.2    Frampton, M.W.3
  • 40
    • 0031464539 scopus 로고    scopus 로고
    • Perforin is activated by a proteolytic cleavage during biosynthesis which reveals a phosphoLipid-binding C2 domain
    • Uellner R., Zvelebil M.J., Hopkins J., Jones J., MacDougall L.K., Morgan B.P., et al. Perforin is activated by a proteolytic cleavage during biosynthesis which reveals a phosphoLipid-binding C2 domain. EMBO J. 1997, 16:7287-7296.
    • (1997) EMBO J. , vol.16 , pp. 7287-7296
    • Uellner, R.1    Zvelebil, M.J.2    Hopkins, J.3    Jones, J.4    MacDougall, L.K.5    Morgan, B.P.6
  • 41
    • 0028935073 scopus 로고
    • Hydrophobic, hydrophiLic and other interactions in epitope-paratope binding
    • Van Oss C.J. Hydrophobic, hydrophiLic and other interactions in epitope-paratope binding. Mol. Immunol. 1995, 32:199-211.
    • (1995) Mol. Immunol. , vol.32 , pp. 199-211
    • Van Oss, C.J.1
  • 42
    • 84907110437 scopus 로고
    • Two methods for the removal of erythrocytes from buffy coats for the production of human leukocyte interferon
    • van Oss C.J., Bronson P.M., Dinolfo E.A., Chadha K.C. Two methods for the removal of erythrocytes from buffy coats for the production of human leukocyte interferon. Immunol. Commun. 1981, 10:549-555.
    • (1981) Immunol. Commun. , vol.10 , pp. 549-555
    • van Oss, C.J.1    Bronson, P.M.2    Dinolfo, E.A.3    Chadha, K.C.4
  • 43
    • 52649113153 scopus 로고    scopus 로고
    • Comparison of three cell fixation methods for high content analysis assays utiLizing quantum dots
    • WilLiams Y., Byrne S., Bashir M., Davies A., Whelan A., Gun'ko Y., et al. Comparison of three cell fixation methods for high content analysis assays utiLizing quantum dots. J. Microsc. 2008, 232:91-98.
    • (2008) J. Microsc. , vol.232 , pp. 91-98
    • WilLiams, Y.1    Byrne, S.2    Bashir, M.3    Davies, A.4    Whelan, A.5    Gun'ko, Y.6
  • 44
    • 84883243212 scopus 로고    scopus 로고
    • Flow cytometric monitoring of residual disease in acute leukemia
    • Wood B.L. Flow cytometric monitoring of residual disease in acute leukemia. Methods Mol. Biol. 2013, 999:123-136.
    • (2013) Methods Mol. Biol. , vol.999 , pp. 123-136
    • Wood, B.L.1
  • 45
    • 82155173476 scopus 로고    scopus 로고
    • Whole blood leukocytes isolation with microfabricated filter for cell analysis
    • Yu L., Warner P., Warner B., Recktenwald D., Yamanishi D., Guia A., et al. Whole blood leukocytes isolation with microfabricated filter for cell analysis. Cytometry A 2011, 79:1009-1015.
    • (2011) Cytometry A , vol.79 , pp. 1009-1015
    • Yu, L.1    Warner, P.2    Warner, B.3    Recktenwald, D.4    Yamanishi, D.5    Guia, A.6
  • 46
    • 85043263093 scopus 로고    scopus 로고
    • Conjugation of Monoclonal Antibodies to Fluorochromes
    • Conjugation of Monoclonal Antibodies to Fluorochromes. http://www.drmr.com/abcon/index.html.
  • 47
    • 85043274142 scopus 로고    scopus 로고
    • Compensation Tutorial
    • Compensation Tutorial. http://www.drmr.com/compensation/index.html.
  • 48
    • 84874604847 scopus 로고    scopus 로고
    • Flow Cytometry - A Basic Introduction
    • Flow Cytometry - A Basic Introduction. http://flowbook.denovosoftware.com.
  • 49
    • 85043297229 scopus 로고    scopus 로고
    • Flow Cytometry and Fluorescence Tutorials
    • Flow Cytometry and Fluorescence Tutorials. http://www.Lifetechnologies.com/us/en/home/support/tutorials.html.
  • 50
    • 85043241166 scopus 로고    scopus 로고
    • Washington State University List and Description of CD Antigens. http://www.vetmed.wsu.edu/tkp/Search.aspx.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.