Flow cytometry and the stability of phycoerythrin-tandem dye conjugates

Cytometry Part A

Volumn 75, Issue 11, 2009, Pages 966-972

  Hulspas, Ruud ^a   Dombkowski, David ^b   Preffer, Frederic ^b   Douglas, Derick ^c   Kildew Shah, Brian ^c   Gilbert, John ^a  

^a Cytonome Inc   (United States)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

^c Sperotech Inc   (United States)

Author keywords

CD4 CD8; Clinical; Compensation; Degradation; Flow cytometry; Fluorochrome; Stability; T regulatory cells

Indexed keywords

DYE; PHYCOERYTHRIN; PHYCOERYTHRIN CY5 CD25; PHYCOERYTHRIN CY5 CD3; PHYCOERYTHRIN CY5 CD38; PHYCOERYTHRIN CY5 CD4; PHYCOERYTHRIN CY5 STREPTAVIDIN; PHYCOERYTHRIN CY7 CD10; PHYCOERYTHRIN CY7 CD19; PHYCOERYTHRIN CY7 CD2; PHYCOERYTHRIN CY7 CD3; PHYCOERYTHRIN CY7 CD4; PHYCOERYTHRIN CY7 CD5; PHYCOERYTHRIN TEXAS RED CD3; PHYCOERYTHRIN TEXAS RED CD4; PHYCOERYTHRIN TEXAS RED CD8; PHYCOERYTHRIN TEXAS RED STREPTAVIDIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DEGRADATION KINETICS; FLOW CYTOMETRY; FLUORESCENCE ANALYSIS; LIGHT EXPOSURE; PRIORITY JOURNAL; STORAGE; THERMOSTABILITY;

BIOTIN; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; CELL SEPARATION; COLORING AGENTS; FLOW CYTOMETRY; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; HUMANS; LEUKOCYTES, MONONUCLEAR; PHYCOERYTHRIN; POLYSTYRENES; T-LYMPHOCYTES; T-LYMPHOCYTES, REGULATORY;

EID: 71049194900     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20799     Document Type: Article

References (20)

1. Schauenstein K, Schauenstein E, Wick G. Fluorescence properties of free and protein bound fluorescein dyes. I. Macrospectrofluorometric measurements. J Histochem Cytochem 1978;26:277-283.
2. Glazer AN, Stryer L. Fluorescent tandem phycobiliprotein conjugates. Emission wavelength shifting by energy transfer. Biophys J 1983;43:383-386.
3. Roederer M, Kantor AB, Parks DR, Herzenberg LA. Cy7PE and Cy7APC: Bright new probes for immunofluorescence. Cytometry 1996;24:191-197. (Pubitemid 126745075)
4. Lansdorp PM, Smith C, Safford M, Terstappen LWMM, Thomas TE. Single laser three color immunofluorescence staining procedures based on energy transfer between phycoerythrin and cyanine 5. Cytometry 1991;12:723-730.
5. Ha T. Single-molecule fluorescence resonance energy transfer. Methods 2001;25:78-86. (Pubitemid 32905290)
6. Szölldo†si J, Damjanovich S, Mátyus L. Application of fluorescence resonance energy transfer in the clinical laboratory: Routine and research. Cytometry 1998;34:159-179. (Pubitemid 28390338)
7. Iqbal A, Arslan S, Okumus B, Wilson TJ, Giraud G, Norman DG, Ha T, Lilley DMJ. Orientation dependence in fluorescent energy transfer between Cy3 and Cy5 terminally attached to double-stranded nucleic acids. Proc Natl Acad Sci USA 2008;105:11176-11181.
8. Stewart CC, Stewart SJ. Four color compensation. Cytometry 1999;38:161-175.
9. Berlier JE, Rothe A, Buller G, Bradford J, Gray DR, Filanoski BJ, Telford WG, Yue S, Liu J, Cheung CY, Chang W, Hirsch JD, Beechem JM, Haugland RP, Haugland RP. Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: Fluorescence of the dyes and their bioconjugates. J Histochem Cytochem 2003;51:1699-1712. (Pubitemid 37475443)
10. Tung JW, Parks DR, Moore WA, Herzenberg LA, Herzenberg LA. New approaches to fluorescence compensation and visualization of FACS data. Clin Immunol 2004;110:277-283.
11. Bayer J, Grunwald D, Lambert C, Mayol JF, Maynadié M. Thematic workshop on fluorescence compensation settings in multicolor flow cytometry. Cytometry Part B 2007;72B:8-13. (Pubitemid 46105452)
12. Forman MA, Gupta RK. Tandem dyes for flow cytometry: Can we overcome quality concerns? Med Lab Obs 2007;39:24-26.
13. Agrawal YP, Mahlamäki EK, Penttilä I. Use of quality control standards in clinical flow cytometry. Ann Med 1991;23:127-133.
14. Zhang Y, Kemper C, Bakke A, Haugland RP. Novel flow cytometry compensation standards: Internally stained fluorescent microspheres with matched emission spectra and long-term stability. Cytometry 1998;33:244-248. (Pubitemid 28447150)
15. Owens MA, Vall HG, Hurley AA, Wormsley SB. Validation and quality control of immunophenotyping in clinical flow cytometry. J Immunol Methods 200;243:33-50.
16. Perfetto SP, Ambrozak D, Nguygen R, Chattopadhyay P, Roederer M. Quality assurance for polychromatic flow cytometry. Nat Protoc 2006;1:1522-1530.
17. Hulspas R, O'Gorman MRG, Wood BL, Gratama, JW, Sutherland DR. Considerations for the control of background fluorescence in clinical flow cytometry. Cytometry Part B 2009;76B:355-364.
18. Galbraith D. Simultaneous flow cytometric quantification of plant nuclear DNA contents over the full range of described angiosperm 2C values. Cytometry Part A 2009;75A:692-698.
19. Pyne S, Hu X, Wang K, Rossin E, Lin TI, Maier LM, Baecher-Allan C, McLachlan GJ, Tamayo P, Hafler DA, De Jager PL, Mesirov JP. Automated high-dimensional flow cytometric data analysis. Proc Natl Acad Sci USA 2009;106:8519-8524.
20. Tholouli E, Sweeney E, Barrow E, Clay V, Hoyland JA, Byers RJ. Quantum dots light up pathology. J Pathol 2008;216:275-285.