Transgenic plants as a sustainable, terrestrial source of fish oils

European Journal of Lipid Science and Technology

Volumn 117, Issue 9, 2015, Pages 1317-1324

  Napier, Johnathan A ^a   Usher, Sarah ^a   Haslam, Richard P ^a   Ruiz Lopez, Noemi ^b   Sayanova, Olga ^a  

^a ROTHAMSTED RESEARCH   (United Kingdom)

^b INSTITUTO DE LA GRASA   (Spain)

Author keywords

Aquaculture; GM plants; Omega 3; Plant biotechnology

Indexed keywords

EID: 84932151744     PISSN: 14387697     EISSN: 14389312     Source Type: Journal    
DOI: 10.1002/ejlt.201400452     Document Type: Review

References (40)

1. Saravanan, P., Davidson, N. C., Schmidt, E. B., Calder, P. C., Cardiovascular effects of marine omega-3 fatty acids. Lancet 2010, 376, 540-550.
2. Tacon, A. G., Metian, M., Global overview on the use of fish meal and fish oil in industrially compounded aquafe eds trends and future prospects. Aquaculture 2008, 285, 146-158.
3. Hixson, S. M., Fish nutrition and current issues in aquaculture: The balance in providing safe and nutritious seafood, in an environmentally sustainable manner. J. Aquac. Res. Devel. 2014, 5, 1-10.
4. Bostock, J., McAndrew, B., Richards, R., Jauncey, K., et al., Aquaculture: Global status and trends. Philos. Trans. R. Soc. Lond. B 2010, 365, 2897-2912.
5. Salem, N., Eggersdorfer, M., Is the world supply of omega-3 fatty acids adequate for optimal human health?. Curr. Opin. Clin. Nutr. Metab. Care 2015, 18, 147-154.
6. Tacon, A. G., Metian, M., Fish matters: Importance of aquatic foods in human nutrition and global food supply. Rev. Fisheries Sci. 2013, 21, 22-38.
7. Henriques, J., Dick, J. R., Tocher, D. R., Bell, J. G., Nutritional quality of salmon products available from major retailers in the UK: Content and composition of n-3 long-chain PUFA. Br. J. Nutr. 2014, 112, 964-975.
8. Nichols, P. D., Glencross, B., Petrie, J. R., Singh, S. P., Readily available sources of long-chain omega-3 oils: Is farmed Australian seafood a better source of the good oil than wild-caught seafood? Nutrients 2014, 6, 1063-1079.
9. Sayanova, O. V., Napier, J. A., Eicosapentaenoic acid: Biosynthetic routes and the potential for synthesis in transgenic plants. Phytochemical 2004, 65, 147-158.
10. Venegas-Calerón, M., Sayanova, O., Napier, J. A., An alternative to fish oils: Metabolic engineering of oil-seed crops to produce omega-3 long chain polyunsaturated fatty acids. Prog. Lipid Res. 2010, 49, 108-119.
11. Petrie, J. R., Singh, S. P., Expanding the docosahexaenoic acid food web for sustainable production: Engineering lower plant pathways into higher plants. AoB Plants 2011, plr 011.
12. Ruiz-Lopez, N, Haslam, RP, Usher, S, Napier, JA, Sayanova, O., An alternative pathway for the effective production of the omega -3 long-chain polyunsaturates EPA and ETA in transgenic oilseeds. Plant Biotechnol. J. 2015, Jan 30 DOI: 10.1111/pbi.12328
13. Metz, J. G., Roessler, P., Facciotti, D., Levering, C., et al., Production of polyunsaturated fatty acids by polyketide synthases in both prokaryotes and eukaryotes. Science 2001, 293, 290-293.
14. McCartney, A. W., Dyer, J. M., Dhanoa, P. K., Kim, P. K., et al., Membrane-bound fatty acid desaturases are inserted co-translationally into the ER and contain different ER retrieval motifs at their carboxy termini. Plant J. 2004, 37, 156-173.
15. Cahoon, E. B., Shockey, J. M., Dietrich, C. R., Gidda, S. K., et al., Engineering oilseeds for sustainable production of industrial and nutritional feedstocks: Solving bottlenecks in fatty acid flux. Curr. Opin. Plant Biol. 2007, 3, 236-244.
16. Ruiz-Lopez, N., Usher, S., Sayanova, O. V., Napier, J. A., Haslam, R. P., Modifying the lipid content and composition of plant seeds: Engineering the production of LC-PUFA. Appl. Microbiol. Biotechnol. 2015, 99, 143-154.
17. Kinney, A. J., Metabolic engineering in plants for human health and nutrition. Curr. Opin. Biotechnol. 2006, 17, 130-138.
18. Napier, J. A., The production of unusual fatty acids in transgenic plants. Annu. Rev. Plant Biol. 2007, 58, 295-319.
19. Qi, B., Fraser, T., Mugford, S., Dobson, G., et al., Production of very long chain polyunsaturated ω-3 and ω-6 fatty acids in plants. Nat. Biotech. 2004, 22, 739-745.
20. Abbadi, A., Domergue, F., Bauer, J., Napier, J. A., et al., Biosynthesis of very-long-chain polyunsaturated fatty acids in transgenic oilseeds: Constraints on their accumulation. Plant Cell. 2004, 16, 2734-2748.
21. Wu, G., Truksa, M., Datla, N., Vrinten, P., et al., Stepwise engineering to produce high yields of very long-chain polyunsaturated fatty acids in plants. Nat. Biotechnol. 2005, 23, 1013-1017.
22. Cheng, B., Wu, G., Vrinten, P., Falk, K., et al., Towards the production of high levels of eicosapentaenoic acid in transgenic plants: The effects of different host species, genes and promoters. Transgenic Res. 2010, 19, 221-229.
23. Petrie, J. R., Shrestha, P., Zhou, X. R., Mansour, M. P., et al., Metabolic engineering plant seeds with fish oil-like levels of DHA. PLoS ONE 2012, 7, 49165.
24. Domergue, F., Abbadi, A., Zähringer, U., Moreau, H., Heinz, E., In vivo characterization of the first acyl-CoA D6-desaturase from a member of the plant kingdom, the microalga Ostreococcus tauri. Biochem. J. 2005, 389, 483-490.
25. Hoffmann, M., Wagner, M., Abbadi, A., Fulda, M., Feussner, I., Metabolic engineering of ω-3 very long chain polyunsaturated fatty acid production by an exclusively acyl-CoA-dependent pathway. J. Biol. Chem. 2008, 283, 22352-22362.
26. Petrie, J. R., Shrestha, P., Mansour, M. P., Nichols, P. D., et al., Metabolic engineering of omega-3 long-chain polyunsaturated fatty acids in plants using an acyl-CoA D6-desaturase with omega3-preference from the marine microalga Micromonas pusilla. Metab. Eng. 2010, 12, 233-240.
27. Sayanova, O., Ruiz-Lopez, N., Haslam, R. P., Napier, J. A., The role of Δ6-desaturase acyl-carrier specificity in the efficient synthesis of long-chain polyunsaturated fatty acids in transgenic plants. Plant Biotechnol. J. 2012, 10, 195-206.
28. Wood, C. C., Petrie, J. R., Shrestha, P., Mansour, M. P., et al., A leaf-based assay using interchangeable design principles to rapidly assemble multistep recombinant pathways. Plant Biotechnol. J. 2009, 7, 914-924.
29. Petrie, J. R., Shrestha, P., Liu, Q., Mansour, M. P., et al., Rapid expression of transgenes driven by seed-specific constructs in leaf tissue: DHA production. Plant Meth. 2010, 6, 8.
30. Ruiz-López, N., Haslam, R. P., Venegas-Calerón, M., Li, T., et al., Enhancing the accumulation of omega-3 long chain polyunsaturated fatty acids in transgenic Arabidopsis thaliana via iterative metabolic engineering and genetic crossing. Transgenic Res. 2012, 21, 1233-1243.
31. Ruiz-Lopez, N., Haslam, R. P., Usher, S. L., Napier, J. A., Sayanova, O., Reconstitution of EPA and DHA biosynthesis in Arabidopsis: iterative metabolic engineering for the synthesis of n-3 LC-PUFAs in transgenic plants. Metab. Eng. 2013, 17, 30-41.
32. Ruiz-Lopez, N., Haslam, R. P., Napier, J. A., Sayanova, O., Successful high-level accumulation of fish oil omega-3 long-chain polyunsaturated fatty acids in a transgenic oilseed crop. Plant J. 2014, 77, 198-208.
33. Petrie, J. R., Shrestha, P., Belide, S., Kennedy, Y., et al., Metabolic engineering Camelina sativa with fish oil-like levels of DHA. PLoS ONE 2014, 9, e85061.
34. Napier, J. A., Haslam, R. P., Beaudoin, F., Cahoon, E. B., Understanding and manipulating plant lipid composition: Metabolic engineering leads the way. Curr. Opin. Plant Biol. 2014, 19, 68-75.
35. Mansour, M. P., Shrestha, P., Belide, S., Petrie, J. R., et al., Characterization of oilseed lipids from "DHA-producing Camelina sativa": A new transformed land plant containing long-chain omega-3 oils. Nutrients 2014, 6, 776-789.
36. Betancor, M. B., Sprague, M., Usher, S., Sayanova, O., et al., A nutritionally-enhanced oil from transgenic Camelina sativa effectively replaces fish oil as a source of eicosapentaenoic acid for fish. Sci. Rep. 2015, 5, 8104.
37. Leaver, M. J., Villeneuve, L. A., Obach, A., Jensen, L., et al., Functional genomics reveals increases in cholesterol biosynthetic genes and highly unsaturated fatty acid biosynthesis after dietary substitution of fish oil with vegetable oils in Atlantic salmon (Salmo salar). BMC Genomics 2008, 24, 299.
38. Hamilton, M. L., Haslam, R. P., Napier, J. A., Sayanova, O., Metabolic engineering of Phaeodactylum tricornutum for the enhanced accumulation of omega-3 long chain polyunsaturated fatty acids. Metab. Eng. 2014, 22, 3-9.
39. Xue, Z., Sharpe, P. L., Hong, S. P., Yadav, N. S., et al., Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica. Nat. Biotechnol. 2013, 31, 734-740.
40. Xie, D., Jackson, E. N., Zhu, Q., Sustainable source of omega-3 eicosapentaenoic acid from metabolically engineered Yarrowia lipolytica: From fundamental research to commercial production. Appl. Microbiol. Biotechnol. 2015, 99, 1599-1610.