Affinity Purification of Protein Complexes Using TAP Tags

Methods in Enzymology

Volumn 559, Issue , 2015, Pages 37-52

  Gerace, Erica ^a   Moazed, Danesh ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

Affinity purification; Calmodulin binding (CAM B); Calmodulin elution (CAM E); IgG Sepharose; Lysis buffer; Protease cleavage; Protein lysates; TAP tags; TEV cleavage (TEV C)

Indexed keywords

FUNGAL PROTEIN; PROTEIN A; PROTEIN HYDROLYSATE; PROTEINASE; CALMODULIN; IMMUNOGLOBULIN G; PROTEIN; PROTEIN BINDING;

ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; ELUTION; EXPRESSED SEQUENCE TAG; FUNGAL GENE; NONHUMAN; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN PURIFICATION; TANDEM AFFINITY PURIFICATION; YEAST CELL; AFFINITY CHROMATOGRAPHY; ANIMAL; CHEMISTRY; DEVICES; HUMAN; ISOLATION AND PURIFICATION; PROCEDURES; PROTEIN ANALYSIS; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES; TANDEM MASS SPECTROMETRY;

TOBACCO ETCH VIRUS;

ANIMALS; CALMODULIN; CHROMATOGRAPHY, AFFINITY; HUMANS; IMMUNOGLOBULIN G; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; PROTEINS; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES; TANDEM MASS SPECTROMETRY;

EID: 84931577319     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/bs.mie.2014.11.007     Document Type: Chapter

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