A novel tandem affinity purification strategy for the efficient isolation and characterisation of native protein complexes

Proteomics

Volumn 7, Issue 23, 2007, Pages 4228-4234

  Gloeckner, Christian Johannes ^a   Boldt, Karsten ^a,b   Schumacher, Annette ^a   Roepman, Ronald ^c   Ueffing, Marius ^a,b  

^a INSTITUTE OF EPIDEMIOLOGY   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^c RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Mass spectrometry; MEK1; Protein complexes; Raf; TAP

Indexed keywords

BIOTIN DERIVATIVE; DETHIOBIOTIN; HEAT SHOCK PROTEIN 70; HYBRID PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; OCTAPEPTIDE; OCTAPEPTIDE FLAG; PROTEIN 14 3 3; RAF PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELL STRAIN HEK293; COMPLEX FORMATION; FUNCTIONAL GENOMICS; HUMAN; INTERMETHOD COMPARISON; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN STABILITY;

14-3-3 PROTEINS; BIOTIN; BLOTTING, WESTERN; CELL LINE; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HUMANS; MAP KINASE KINASE 1; MULTIPROTEIN COMPLEXES; OLIGOPEPTIDES; PEPTIDES; PROTO-ONCOGENE PROTEINS B-RAF; TANDEM MASS SPECTROMETRY; TRANSFECTION;

MAMMALIA;

EID: 37048999786     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.200700038     Document Type: Article

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