Scaffold/matrix attachment regions from CHO cell chromosome enhanced the stable transfection efficiency and the expression of transgene in CHO cells

Biotechnology and Applied Biochemistry

Volumn 61, Issue 5, 2014, Pages 510-516

  Chang, Ming ^a   Liu, Ruijie ^a   Jin, Qingzhe ^a   Liu, Yuanfa ^a   Wang, Xingguo ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Chinese hamster ovary cells; high expression; integration efficiency; scaffold matrix attachment region

Indexed keywords

ANIMALS; CELLS; CHROMOSOMES; CLONING; CYTOLOGY; EFFICIENCY; ENZYMES; GENES; MOLECULAR BIOLOGY; SCAFFOLDS (BIOLOGY);

CHINESE HAMSTER OVARY CELLS; DOMAIN BOUNDARY; EXPRESSION VECTORS; HETEROLOGOUS GENES; HIGH EXPRESSION; LYSOZYME GENES; TRANSFECTION EFFICIENCY; TRANSGENE;

GENE EXPRESSION;

LYSOZYME; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; CHO CELL LINE; CHROMOSOME; CONTROLLED STUDY; DNA SEQUENCE; EXPRESSION VECTOR; GENE DOSAGE; GENE EXPRESSION; MATRIX ATTACHMENT REGION; NONHUMAN; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; STABLE TRANSFECTION; TRANSGENE; ANIMAL; COMPUTER SIMULATION; CRICETULUS; GENETIC TRANSFECTION; GENETICS; GENOMIC INSTABILITY; HAMSTER; METABOLISM; PROCEDURES;

CRICETULUS GRISEUS;

ANIMALS; CHO CELLS; COMPUTER SIMULATION; CRICETINAE; CRICETULUS; GENE DOSAGE; GENOMIC INSTABILITY; MATRIX ATTACHMENT REGIONS; RECOMBINANT PROTEINS; TRANSFECTION; TRANSGENES;

EID: 84931389834     PISSN: 08854513     EISSN: 14708744     Source Type: Journal    
DOI: 10.1002/bab.1204     Document Type: Article

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