Nanohydroxyapatite-reinforced chitosan composite hydrogel for bone tissue repair in vitro and in vivo

Journal of Nanobiotechnology

Volumn 13, Issue 1, 2015, Pages

  Dhivya, S ^a   Saravanan, S ^a   Sastry, T P ^b   Selvamurugan, N ^a  

^a SRM INSTITUTE OF SCIENCE AND TECHNOLOGY   (India)

^b CENTRAL LEATHER RESEARCH INSTITUTE   (India)

Author keywords

Bone; Chitosan; nHAp; Runx2; Zinc; Glycerophosphate

Indexed keywords

BIOMINERALIZATION; BONE; CELL CULTURE; CHITIN; CHITOSAN; DEFECTS; ENERGY DISPERSIVE SPECTROSCOPY; FOURIER TRANSFORM INFRARED SPECTROSCOPY; GENE EXPRESSION; MOBILE SECURITY; PHOSPHATE MINERALS; PHOSPHOLIPIDS; POLYMERASE CHAIN REACTION; PROTEINS; SCAFFOLDS (BIOLOGY); SCANNING ELECTRON MICROSCOPY; SOL-GEL PROCESS; SOL-GELS; STEM CELLS; TISSUE; TISSUE ENGINEERING; TISSUE REGENERATION; X RAY DIFFRACTION; ZINC;

GLYCEROPHOSPHATE; NHAP; OSTEOBLAST DIFFERENTIATION; REVERSE-TRANSCRIPTASE POLYMERASE CHAIN REACTIONS; RUNX2; THERMO-SENSITIVE HYDROGEL; THREEDIMENSIONAL (3-D); WESTERN-BLOT ANALYSIS;

HYDROGELS;

APATITE; CHITOSAN; COLLAGEN; GLYCEROL 2 PHOSPHATE; HYDROXYAPATITE; ZINC; ANTIINFECTIVE AGENT; BETA-GLYCEROPHOSPHORIC ACID; GLYCEROPHOSPHATE; HYDROGEL; NANOCOMPOSITE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIMICROBIAL ACTIVITY; ARTICLE; BIOMINERALIZATION; BONE DEFECT; BONE DEVELOPMENT; BONE REMODELING; CELL DIFFERENTIATION; CONTROLLED STUDY; CYTOTOXICITY; DRUG FORMULATION; DRUG STRUCTURE; DRUG SYNTHESIS; ESCHERICHIA COLI; GENE EXPRESSION; HYDROGEL; IN VITRO STUDY; IN VIVO STUDY; MALE; MESENCHYMAL STEM CELL; MOUSE; NANOBIOTECHNOLOGY; NONHUMAN; OSSIFICATION; OSTEOBLAST; RAT; STREPTOCOCCUS PYOGENES; ANIMAL; BONE REGENERATION; CHEMISTRY; CYTOLOGY; DRUG EFFECTS; INFRARED SPECTROSCOPY; MATERIALS TESTING; MESENCHYMAL STROMA CELL; PHARMACOLOGY; PHYSIOLOGY; PROCEDURES; SCANNING ELECTRON MICROSCOPY; TIBIA; WISTAR RAT; X RAY DIFFRACTION;

RATTUS;

ANIMALS; ANTI-BACTERIAL AGENTS; BONE REGENERATION; CELL DIFFERENTIATION; CHITOSAN; DURAPATITE; GLYCEROPHOSPHATES; HYDROGEL; MALE; MATERIALS TESTING; MESENCHYMAL STROMAL CELLS; MICE; MICROSCOPY, ELECTRON, SCANNING; NANOCOMPOSITES; OSTEOBLASTS; OSTEOGENESIS; RATS, WISTAR; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; TIBIA; X-RAY DIFFRACTION; ZINC;

EID: 84931264437     PISSN: None     EISSN: 14773155     Source Type: Journal    
DOI: 10.1186/s12951-015-0099-z     Document Type: Article

References (52)

1. Arrington ED, Smith WJ, Chambers HG, Bucknell AL, Davino NA (1996) Complications of iliac crest bone graft harvesting. Clin Orthop Relat Res 329:300-309
2. Schaaf H, Lendeckel S, Howaldt HP, Streckbein P (2010) Donor site morbidity after bone harvesting from the anterior iliac crest. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 109:52-58
3. Drury JL, Mooney DJ (2003) Hydrogels for tissue engineering: scaffold design variables and applications. Biomaterials 24:4337-4351
4. Lee KY, Mooney DJ (2001) Hydrogels for tissue engineering. Chem Rev 101:1869-1880
5. Fedorovich NE, Alblas J, De Wijn JR, Hennink WE, Verbout AJ, Dhert WJ (2007) Hydrogels as extracellular matrices for skeletal tissue engineering: state-of-the-art and novel application in organ printing. Tissue Eng 13:1905-1925
6. Mano JF (2008) Stimuli-responsive polymeric systems for biomedical applications. Adv Eng Mater 10:515-527
7. Huang Z, Tian J, Yu B, Xu Y, Feng Q (2009) A bone-like nano-hydroxyapatite/collagen loaded injectable scaffold. Biomed Mater. doi: 10.1088/1748-6041/4/5/055005
8. Martins AM, Alves CM, Kasper FK, Mikos AG, Reis RL (2010) Responsive and in situ-forming chitosan scaffolds for bone tissue engineering applications: an overview of the last decade. J Mater Chem 20:1638-1645
9. Jayakumar R, Prabaharan M, Nair SV, Tokura S, Tamura H, Selvamurugan N (2010) Novel carboxymethyl derivatives of chitin and chitosan materials and their biomedical applications. Prog Mater Sci 55:675-709
10. Knapczyk J, Krowczynski L, Krzck J, Brzeski M, Nirnberg E, Schenk D et al (1989) Requirements of chitosan for pharmaceutical and biomedical applications. In: Skjåk-Braek G, Anthonsen T, Sandford PA (eds) Chitin and Chitosan: sources, chemistry, biochemistry, physical properties and applications. Elsevier, London, pp 657-663
11. Hirano S, Seino H, Akiyama I, Nonaka I (1990) Chitosan: a biocompatible material for oral and intravenous administration. In: Gebelein CG, Dunn RL (eds) Progress in biomedical polymers. Plenum Press, New York, pp 283-90
12. Muzzarelli RAA (1997) Human enzymatic activities related to the therapeutic administration of chitin derivatives. Cell Mol Life Sci 53:131-140
13. Raafat D, Sahl HG (2009) Chitosan and its antimicrobial potential-a critical literature survey. Microb Biotechnol 2:186-201
14. Freier T, Koh HS, Kazazian K, Shoichet MS (2005) Controlling cell adhesion and degradation of chitosan films by N-acetylation. Biomaterials 26:5872-5878
15. Madihally SV, Matthew HWT (1999) Porous chitosan scaffolds for tissue engineering. Biomaterials 20:1133-1142
16. Varma AJ, Deshpande SV, Kennedy JF (2004) Metal complexation by chitosan and its derivatives: a review. Carbohydr Pol. 55:77-93
17. Rohindra R, Nand AV, Khurma JR (2004) Swelling properties of chitosan hydrogels. South Pac J Nat App Sci. 22:32-35
18. Qiu Y, Park K (2001) Environment-sensitive hydrogels for drug delivery. Adv Drug Deliv Rev 53:321-339
19. Chvapil M (1973) New aspects in the biological role of zinc: a stabilizer of macromolecules and biological membranes. Life Sci 13:1041-1049
20. Yamaguchi M, Weitzmann MN (2011) Zinc stimulates osteoblastogenesis and suppresses osteoclastogenesis by antagonizing NF-ΚB activation. Mol Cell Biochem 355:179-186
21. Hershfinkel M, Moran A, Grossman N, Sekler J (2001) A zinc-sensing receptor triggers the release of intracellular Ca2+ and regulates ion transport. Proc Natl Acad Sci USA 20:11749-11754
22. Tripathi A, Saravanan S, Pattnaik S, Moorthi A, Partridge NC, Selvamurugan N (2012) Bio-composite scaffolds containing chitosan/nano-hydroxyapatite/nano-copper-zinc for bone tissue engineering. Int J Biol Macromol 50:294-299
23. Zhou H, Lee J (2011) Nanoscale hydroxyapatite particles for bone tissue engineering. Acta Biomater 7:2769-2781
24. Webster TJ, Ergun C, Doremus RH, Siegel RW, Bizios R (2000) Specific proteins mediate enhanced osteoblast adhesion on nanophase ceramics. J Biomed Mat Res 51:475-483
25. Gleeson JP, Plunkett NA (2010) Addition of hydroxyapatite improves stiffness, interconnectivity and osteogenic potential of a highly porous collagen-based scaffold for bone tissue regeneration. Eur Cell Mater 20:218-230
26. Chung H, Golub EE, Forbes E, Tokuoka T, Shapiro IM (1992) Mechanism of action of beta-glycerophosphate on bone cell mineralization. Calcif Tissue Int 51:305-311
27. Chang YL, Stanford CM, Keller JC (2000) Calcium and phosphate supplementation promotes bone cell mineralization: implications for hydroxyapatite (HA)-enhanced bone formation. J Biomed Mat Res 52:270-278
28. Wang L, Stegemann JP (2010) Thermogelling chitosan and collagen composite hydrogels initiated with Β-glycerophosphate for bone tissue engineering. Biomaterials 31:3976-3985
29. Cho J, Heuzey MC, Begin A, Carreau PJ (2005) Physical gelation of chitosan in the presence of Β-glycerophosphate: the effect of temperature. Biomacromolecules 6:3267-3275
30. Lavertu M, Filion D, Buschmann MD (2008) Heat-induced transfer of protons from chitosan to glycerol phosphate produces chitosan precipitation and gelation. Biomacromolecules 79:640-650
31. Chenite M, Buschmann D, Wang D, Chaput C, Kandani N (2001) Rheological characterization of thermogelling chitosan/glycerol phosphate solutions. Carbohydr Polym 46:39-47
32. Huang J, Best SM, Bonfield W, Brooks RA, Rushton N, Jayasinghe SN et al (2004) In vitro assessment of the biological response to nano-sized hydroxyapatite. J Mater Sci Mater Med 15:441-445
33. Cerroni L, Filocamo R, Fabbri M, Piconi C, Caropreso S, Condo SG (2002) Growth of osteoblast-like cells on porous hydroxyapatite ceramics: an in vitro study. Biomol Eng 19:119-124
34. Zhou H, Lee J (2011) Nanoscale hydroxyapatite particles for bone tissue engineering. Acta Biomater 7:2762-2781
35. Salehi R, Arami M, Mahmoodi NM, Bahrami H, Khorramfar S (2010) Novel biocompatible composite (chitosan-zinc oxide nanoparticle): preparation, characterization and dye adsorption properties. Colloids Surf B Biointerfaces 80:86-93
36. Wang X, Du Y, Fan L, Liu H, Hu Y (2005) Chitosan-metal complexes as antimicrobial agent: synthesis, characterization and structure-activity study. Polym Bull 55:105-113
37. Han JK, Song HY, Saito F, Lee BT (2006) Synthesis of high purity nano-sized hydroxyapatite powder by microwave-hydrothermal method. Mater Chem Phy 99:235-239
38. Balamurugan A, Michel J, Faure J, Benhayoune H, Wortham L, Sockalingum G et al (2006) Synthesis and structural analysis of sol gel derived stoichiometric monophasic hydroxyapatite. Ceram Silik 50:27-31
39. Pawlak A, Mucha M (2003) Thermogravimetric and FTIR studies of chitosan blends. Thermochim Acta 396:153-166
40. Peter M, Ganesh N, Selvamurugan N, Nair SV, Furuike T, Tamura H et al (2010) Preparation and characterization of chitosan-gelatin/nanohydroxyapatite composite scaffolds for tissue engineering applications. Carbohydr Poly 80:687-694
41. Ji C, Khademhosseini A, Dehghani F (2011) Enhancing cell penetration and proliferation in chitosan hydrogels for tissue engineering applications. Biomaterials 32:9719-9729
42. Ren D, Yi H, Wang W, Ma X (2005) The enzymatic degradation and swelling properties of chitosan matrices with different degrees of N-acetylation. Carbohydr Res 340:2403-2410
43. Lord MS, Foss M, Besenbacher F (2010) Influence of nanoscale surface topography on protein adsorption and cellular response. Nano Today 5:66-78
44. Harada H, Tagashira S, Fujiwara M, Ogawa S, Katsumata T, Yamaguchi A et al (1999) Cbfa1 isoforms exert functional differences in osteoblast differentiation. J Biol Chem 274:6972-6978
45. Bellows CG, Aubin JE, Heersche JNM (1991) Initiation and progression of mineralization of bone nodules formed in vitro: the role of alkaline phosphatase and organic phosphate. Bone Min 14:27-40
46. Nudelman F, Pieterse K, George A, Bomans PH, Friedrich H, Brylka LJ et al (2010) The role of collagen in bone apatite formation in the presence of hydroxyapatite nucleation inhibitors. Nat Mater 9:1004-1009
47. Boskey AL, Gadaleta S, Gundberg C, Doty SB, Ducy P, Karsenty G (1998) Fourier transform infrared microspectroscopic analysis of bones of osteocalcin-deficient mice provides insight into the function of osteocalcin. Bone 23:187-196
48. Niranjan R, Koushik C, Saravanan S, Moorthi A, Vairamani M, Selvamurugan N (2012) A novel injectable temperature-sensitive zinc doped chitosan/Β-glycerophosphate hydrogel for bone tissue engineering. Int J Biol Macromol 54:24-29
49. Pattnaik S, Nethala S, Tripathi A, Saravanan S, Moorthi A, Selvamurugan N (2011) Chitosan scaffolds containing silicon dioxide and zirconia nano particles for bone tissue engineering. Int J Biol Macromol 49:1167-1172
50. Jalota S, Bhaduri SB, Tas AC (2008) Using a synthetic body fluid (SBF) solution of 27mM HCO3 - to make bone substitutes more osteointegrative. Mater Sci Eng C 28:129-140
51. Moller B, Terheyden H, Açil Y, Purcz NM, Hertrampf K, Tabakov A et al (2012) A comparison of biocompatibility and osseointegration of ceramic and titanium implants: an in vivo and in vitro study. Int J Oral Maxillofac Surg 41:638-645
52. Vimalraj S, Partridge NC, Selvamurugan N (2014) A positive role of microRNA-15b on regulation of osteoblast differentiation. J Cell Physiol 229:1236-1244