Alpha or beta human chorionic gonadotropin knockdown decrease BeWo cell fusion by down-regulating PKA and CREB activation

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Malhotra, Sudha Saryu ^a   Suman, Pankaj ^b   Gupta, Satish Kumar ^a  

^a NATIONAL INSTITUTE OF IMMUNOLOGY   (India)

^b AMITY UNIVERSITY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA CHAIN; BETA CATENIN; CGA PROTEIN, HUMAN; CHORIONIC GONADOTROPIN BETA SUBUNIT; CREB1 PROTEIN, HUMAN; CYCLIC AMP DEPENDENT PROTEIN KINASE; CYCLIC AMP RESPONSIVE ELEMENT BINDING PROTEIN; FORSKOLIN; ISOQUINOLINE DERIVATIVE; MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE P38; N-(2-(4-BROMOCINNAMYLAMINO)ETHYL)-5-ISOQUINOLINESULFONAMIDE; PLACENTA PROTEIN; PROTEIN KINASE INHIBITOR; SDC1 PROTEIN, HUMAN; SMALL INTERFERING RNA; SULFONAMIDE; SYNCYTIN; SYNDECAN 1; VIRUS ENVELOPE PROTEIN;

ALPHA CHAIN; ANTAGONISTS AND INHIBITORS; BIOSYNTHESIS; CELL FUSION; DOWN REGULATION; ENZYME ACTIVATION; GENETICS; HUMAN; METABOLISM; PHOSPHORYLATION; PHYSIOLOGY; RNA INTERFERENCE; TROPHOBLAST;

BETA CATENIN; CELL FUSION; CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN; COLFORSIN; CYCLIC AMP RESPONSE ELEMENT-BINDING PROTEIN; CYCLIC AMP-DEPENDENT PROTEIN KINASES; DOWN-REGULATION; ENZYME ACTIVATION; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; GENE PRODUCTS, ENV; GLYCOPROTEIN HORMONES, ALPHA SUBUNIT; HUMANS; ISOQUINOLINES; P38 MITOGEN-ACTIVATED PROTEIN KINASES; PHOSPHORYLATION; PREGNANCY PROTEINS; PROTEIN KINASE INHIBITORS; RNA INTERFERENCE; RNA, SMALL INTERFERING; SULFONAMIDES; SYNDECAN-1; TROPHOBLASTS;

EID: 84930960148     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep11210     Document Type: Article

References (41)

1. Red-Horse, K. et al. Trophoblast differentiation during embryo implantation and formation of the maternal-fetal interface. J Clin Invest. 114, 744-754 (2004
2. Langbein, M. et al. Impaired cytotrophoblast cell-cell fusion is associated with reduced Syncytin and increased apoptosis in patients with placental dysfunction. Mol Reprod Dev. 75, 175-183 (2008
3. Gauster, M., Moser, G., Orendi, K. & Huppertz, B. Factors involved in regulating trophoblast fusion: potential role in the development of preeclampsia. Placenta 30, S49-S54 (2009
4. Morrish, D. W., Bhardwaj, D., Dabbagh, L. K., Marusyk, H. & Siy, O. Epidermal growth factor induces differentiation and secretion of human chorionic gonadotropin and placental lactogen in normal human placenta. J Clin Endocrinol Metab. 65, 1282-1290 (1987
5. Garcia-Lloret, M. I. et al. Demonstration of functional cytokine-placental interactions: CSF-1 and GM-CSF stimulate human cytotrophoblast differentiation and peptide hormone secretion. Exp Cell Res. 214, 46-54 (1994
6. Sawai, K. et al. Leukemia inhibitory factor (LIF) enhances trophoblast differentiation mediated by human chorionic gonadotropin (hCG). Biochem Biophys Res Commun. 211, 137-143 (1995
7. Morrish, D. W., Bhardwaj, D. & Paras, M. T. Transforming growth factor beta 1 inhibits placental differentiation and human chorionic gonadotropin and human placental lactogen secretion. Endocrinology 129, 22-26 (1991
8. Mi, S. et al. Syncytin is a captive retroviral envelope protein involved in human placental morphogenesis. Nature 403, 785-789 (2000
9. Marin, M., Lavillette, D., Kelly, S. M. & Kabat, D. N-linked glycosylation and sequence changes in a critical negative control region of the ASCT1 and ASCT2 neutral amino acid transporters determine their retroviral receptor functions. J Virol. 77, 2936-2945 (2003
10. Cronier, L., Herv J. C., De, J. & Malassin A. Regulation of gap junctional communication during human trophoblast differentiation. Microsc Res Tech. 38, 21-28 (1997
11. Kudo, Y., Boyd, C. A., Millo, J., Sargent, I. L. & Redman, C. W. Manipulation of CD98 expression affects both trophoblast cell fusion and amino acid transport activity during syncytialization of human placental BeWo cells. J Physiol. 550, 3-9 (2003
12. Dalton, P., Christian, H. C., Redman, C. W., Sargent, I. L. & Boyd, C. A. Membrane trafficking of CD98 and its ligand galectin 3 in BeWo cells-implication for placental cell fusion. FEBS J. 274, 2715-2727 (2007
13. Prakash, G. J., Suman, P. & Gupta, S. K. Relevance of syndecan-1 in the trophoblastic BeWo cell syncytialization. Am J Reprod Immunol. 66, 385-393 (2011
14. Shi, Q. J., Lei, Z. M., Rao, C. V. & Lin, J. Novel role of human chorionic gonadotropin in differentiation of human cytotrophoblasts. Endocrinology 132, 1387-1395 (1993
15. Yang, M., Lei, Z. M. & Rao, Ch.V. The central role of human chorionic gonadotropin in the formation of human placental syncytium. Endocrinology 144, 1108-1120 (2003
16. Pidoux, G. et al. Human placental development is impaired by abnormal human chorionic gonadotropin signaling in trisomy 21 pregnancies. Endocrinology 148, 5403-5413 (2007
17. Pidoux, G. et al. Review: Human trophoblast fusion and differentiation: lessons from trisomy 21 placenta. Placenta 33, S81-S86 (2012
18. Themmen, A. P. N. & Huhtaniemi, I. T. Mutations of gonadotropins and gonadotropin receptors: elucidating the physiology and pathophysiology of pituitary-gonadal function. Endocr Rev. 21, 551-583 (2000
19. Vassart, G., Pardo, L. & Costagliola, S. A molecular dissection of the glycoprotein hormone receptors. Trends Biochem Sci. 29, 119-126 (2004
20. Knerr, I. et al. Stimulation of GCMa and syncytin via cAMP mediated PKA signaling in human trophoblastic cells under normoxic and hypoxic conditions. FEBS Lett. 579, 3991-3998 (2005
21. Johnstone, E. D., Sibley, C. P., Lowen, B. & Guilbert, L. J. EGF stimulation of trophoblast differentiation requires MAPK11/14 (p38 MAP kinase) activation. Biol Reprod. 73, 1282-1288 (2005
22. Leduc, K. et al. Leukemia inhibitory factor regulates differentiation of trophoblast like BeWo cells through the activation of JAK/ STAT and MAPK3/1 MAP kinase-signaling pathways. Biol Reprod. 86, 54 (2012
23. Delidaki, M., Gu, M., Hein, A., Vatish, M. & Grammatopoulos, D. K. Interplay of cAMP and MAPK pathways in hCG secretion and fusogenic gene expression in a trophoblast cell line. Mol Cell Endocrinol. 332, 213-220 (2011
24. Matsuura, K. et al. Identification of a link between Wnt/ -catenin signalling and the cell fusion pathway. Nat Commun. 2, 548 (2011
25. Drewlo, S., Baczyk, D., Dunk, C. & Kingdom, J. Fusion assays and models for the trophoblast. Methods Mol Biol. 475, 363-382 (2008
26. Orendi, K., Gauster, M., Moser, G., Meiri, H. & Huppertz, B. The choriocarcinoma cell line BeWo: syncytial fusion and expression of syncytium-specific proteins. Reproduction 140, 759-766 (2010
27. Handwerger, S. New insights into the regulation of human cytotrophoblast cell differentiation. Mol Cell Endocrinol. 323, 94-104 (2010
28. Rao, C. V. Multiple novel roles of luteinizing hormone. Fertil. Steril. 76, 1097-1100 (2001
29. Keryer, G., Alsat, E., Tasken, K. & Evain-Brion, D. Cyclic AMP-dependent protein kinases and human trophoblast cell differentiation in vitro. J Cell Sci. 111, 995-1004 (1998
30. Douglas, G. C. & King, B. F. Differentiation of human trophoblast cells in vitro as revealed by immunocytochemical staining of desmoplakin and nuclei. J Cell Sci. 96, 131-141 (1990
31. Jokimaa, V. I., Kujari, H. P., Ekholm, E. M., Inki, P. L. & Anttila, L. Placental expression of syndecan 1 is diminished in preeclampsia. Am J Obstet Gynecol. 183, 1495-1498 (2000
32. Daoud, G. et al. ERK1/2 and p38 regulate trophoblasts differentiation in human term placenta. J Physiol. 566, 409-423 (2005
33. Zhang, Z., Zhang, L., Jia, L., Wang, P. & Gao, Y. Association of Wnt2 and sFRP4 expression in the third trimester placenta in women with severe preeclampsia. Reprod Sci. 20, 981-989 (2013
34. Zhang, Z., Li, H., Zhang, L., Jia, L. & Wang, P. Differential expression of -catenin and Dickkopf-1 in the third trimester placentas from normal and preeclamptic pregnancies: a comparative study. Reprod Biol Endocrinol. 4, 11-17 (2013
35. Sonderegger, S., Husslein, H., Leisser, C. & Knler, M. Complex expression pattern of Wnt ligands and frizzled receptors in human placenta and its trophoblast subtypes. Placenta 28, S97-S102 (2007
36. Hino, S., Tanji, C., Nakayama, K. I. & Kikuchi, A. Phosphorylation of beta-catenin by cyclic AMP-dependent protein kinase stabilizes beta-catenin through inhibition of its ubiquitination. Mol Cell Biol. 25, 9063-9072 (2005
37. Roy, L. et al. Convergence of 3', 5'-cyclic adenosine 5'-monophosphate/protein kinase A and glycogen synthase kinase-3beta/beta-catenin signaling in corpus luteum progesterone synthesis. Endocrinology 150, 5036-5045 (2009
38. Taurin, S., Sandbo, N., Qin, Y., Browning, D. & Dulin, N. O. Phosphorylation of beta-catenin by cyclic AMP-dependent protein kinase. J Biol Chem. 281, 9971-9976 (2006
39. Suman, P., Shembekar, N. & Gupta, S. K. Leukemia inhibitory factor increases the invasiveness of trophoblastic cells through integrated increase in the expression of adhesion molecules and pappalysin 1 with a concomitant decrease in the expression of tissue inhibitor of matrix metalloproteinases. Fertil Steril. 99, 533-542 (2013
40. Gupta, S. K., Singh, O., Kaur, J. & Talwar, G. P. Characterization of monoclonal anti-alpha human chorionic gonadotropin antibody. Indian J Med Res. 81, 281-285 (1985
41. Gupta, S. K. & Talwar, G. P. Development of hybridomas secreting anti-human chorionic gonadotropin antibodies. Indian J Exp Biol. 18, 1361-1365 (1980