Sensitive detection of thirteen bacterial vaginosis-associated agents using multiplex polymerase chain reaction

BioMed Research International

Volumn 2015, Issue , 2015, Pages

  Malaguti, Natália ^a   Bahls, Larissa Danielle ^a   Uchimura, Nelson Shozo ^a   Gimenes, Fabrícia ^a   Consolaro, Marcia Edilaine Lopes ^a  

^a STATE UNIVERSITY OF MARINGÁ   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; BACTERIAL DNA;

ARTICLE; ATOPOBIUM VAGINAE; BACTERIUM DETECTION; BACTEROIDES FRAGILIS; CLOSTRIDIA LIKE BACTERIUM; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; DNA EXTRACTION; FEMALE; GARDNERELLA VAGINALIS; HUMAN; MEGASPHAERA; MOBILUNCUS CURTISII; MOBILUNCUS MULIERIS; MULTIPLEX POLYMERASE CHAIN REACTION; MYCOPLASMA GENITALIUM; MYCOPLASMA HOMINIS; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; SENSITIVITY AND SPECIFICITY; SNEATHIA SANGUINEGENS; UREAPLASMA UREALYTICUM; VAGINITIS; ACTINOBACTERIA; ANAEROBIC BACTERIUM; GENETICS; ISOLATION AND PURIFICATION; LACTOBACILLUS; MICROBIOLOGY; PATHOLOGY; SPECIES DIFFERENCE; VAGINA; VAGINOSIS, BACTERIAL;

ATOPOBIUM VAGINAE; BACTERIA (MICROORGANISMS); BACTEROIDES FRAGILIS; CLOSTRIDIA; GARDNERELLA VAGINALIS; MEGASPHAERA; MOBILUNCUS CURTISII; MOBILUNCUS MULIERIS; MYCOPLASMA GENITALIUM; MYCOPLASMA HOMINIS; SNEATHIA SANGUINEGENS; UREAPLASMA UREALYTICUM;

ACTINOBACTERIA; BACTERIA, ANAEROBIC; DNA, BACTERIAL; FEMALE; HUMANS; LACTOBACILLUS; MULTIPLEX POLYMERASE CHAIN REACTION; SPECIES SPECIFICITY; VAGINA; VAGINOSIS, BACTERIAL;

EID: 84930649289     PISSN: 23146133     EISSN: 23146141     Source Type: Journal    
DOI: 10.1155/2015/645853     Document Type: Article

References (53)

1. J. R. Schwebke, "New concepts in the etiology of bacterial vaginosis," Current Infectious Disease Reports, vol. 11, no. 2, pp. 143-147, 2009.
2. J. M. Marrazzo, "Interpreting the epidemiology and natural history of bacterial vaginosis: are we still confused?" Anaerobe, vol. 17, no. 4, pp. 186-190, 2011.
3. M. A. Klebanof, J. R. Schwebke, J. Zhang, T. R. Nansel, K.-F. Yu, and W. W. Andrews, "Vulvovaginal symptoms in women with bacterial vaginosis," Obstetrics & Gynecology, vol. 104, no. 2, pp. 267-272, 2004.
4. E. Gillet, J. F. A. Meys, H. Verstraelen et al., "Bacterial vaginosis is associated with uterine cervical human papillomavirus infection: a meta-analysis," BMC Infectious Diseases, vol. 11, article 10, 2011.
5. P. Hay, "Bacterial vaginosis," Medicine, vol. 42, no. 7, pp. 359-363, 2014.
6. J. Ravel, P. Gajer, Z. Abdo et al., "Vaginal microbiome of reproductive-age women," Proceedings of the National Academy of Sciences of the United States of America, vol. 108, supplement 1, pp. 4680-4687, 2011.
7. J. F. Peipert, R. B. Ness, J. Blume et al., "Clinical predictors of endometritis in women with symptoms and signs of pelvic infammatory disease," American Journal of Obstetrics & Gyne-cology, vol. 184, no. 5, pp. 856-864, 2001.
8. C. L. Haggerty, S. L. Hillier, D. C. Bass, and R. B. Ness, "Bacterial vaginosis and anaerobic bacteria are associated with endometritis," Clinical Infectious Diseases, vol. 39, no. 7, pp. 990-995, 2004.
9. M. A. Klebanof, S. L. Hillier, R. P. Nugent et al., "Is bacterial vaginosis a stronger risk factor for preterm birth when it is diagnosed earlier in gestation?" American Journal of Obstetrics & Gynecology, vol. 192, no. 2, pp. 470-477, 2005.
10. Z. Ling, J. Kong, F. Liu et al., "Molecular analysis of the diversity of vaginal microbiota associated with bacterial vaginosis," BMC Genomics, vol. 11, no. 1, article 488, 2010.
11. H. M. Silver, R. S. Sperling, P. J. St. Clair, and R. S. Gibbs, "Evidence relating bacterial vaginosis to intraamniotic infection," American Journal of Obstetrics & Gynecology, vol. 161, no. 3, pp. 808-812, 1989.
12. S. L. Hillier, J. Martius, M. Krohn, N. Kiviat, K. K. Holmes, and D. A. Eschenbach, "A case-control study of chorioamnionic infection and histologic chorioamnionitis in prematurity," The New England Journal of Medicine, vol. 319, no. 15, pp. 972-978, 1988.
13. H. Leitich, B. Bodner-Adler, M. Brunbauer, A. Kaider, C. Egarter, and P. Husslein, "Bacterial vaginosis as a risk factor for preterm delivery: a meta-analysis," American Journal of Obstetrics & Gynecology, vol. 189, no. 1, pp. 139-147, 2003.
14. R. L. Sweet, "Gynecologic conditions and bacterial vaginosis: implications for the non-pregnant patient," Infectious Diseases in Obstetrics and Gynecology, vol. 8, no. 3-4, pp. 184-190, 2000.
15. A. H. N. Ugwumadu, "Bacterial vaginosis in pregnancy," Current Opinion in Obstetrics and Gynecology, vol. 14, no. 2, pp. 115-118, 2002.
16. C. R. Cohen, S. C. Cheng, S. Shiboski et al., "Diaphragm used with replens gel and risk of bacterial vaginosis: results from a randomized controlled trial," Infectious Diseases in Obstetrics and Gynecology, vol. 2012, Article ID 921519, 8 pages, 2012.
17. R. Chawla, P. Bhalla, S. Chadha, S. Grover, and S. Garg, "Comparison of Hay's criteria with Nugent's scoring system for diagnosis of bacterial vaginosis," BioMed Research International, vol. 2013, Article ID 365194, 5 pages, 2013.
18. R. M. Brotman and J. Ravel, "Ready or not: the molecular diagnosis of bacterial vaginosis," Clinical Infectious Diseases, vol. 47, no. 1, pp. 44-46, 2008.
19. R. Amsel, P. A. Totten, C. A. Spiegel, K. C. Chen, D. Eschenbach, and K. K. Holmes, "Nonspecifc vaginitis. Diagnostic criteria and microbial and epidemiologic associations," The American Journal of Medicine, vol. 74, no. 1, pp. 14-22, 1983.
20. R. P. Nugent, M. A. Krohn, and S. L. Hillier, "Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation," Journal of Clinical Microbiology, vol. 29, no. 2, pp. 297-301, 1991.
21. H. Bahar, M. M. Torun, F. Öçer, and B. Kocazeybek, "Mobilun-cus species in gynaecological and obstetric infections: antimicrobial resistance and prevalence in a Turkish population," International Journal of Antimicrobial Agents, vol. 25, no. 3, pp. 268-271, 2005.
22. R. T. Gergova, T. V. Strateva, and I. G. Mitov, "Gardnerella vaginalis-associated bacterial vaginosis in Bulgarian women," Brazilian Journal of Infectious Diseases, vol. 17, no. 3, pp. 313-318, 2013.
23. C. P. Cartwright, B. D. Lembke, K. Ramachandran et al., "Development and validation of a semiquantitative, multitarget PCR assay for diagnosis of bacterial vaginosis," Journal of Clinical Microbiology, vol. 50, no. 7, pp. 2321-2329, 2012.
24. J.-P. Menard, F. Fenollar, M. Henry, F. Bretelle, and D. Raoult, "Molecular quantifcation of Gardnerella vaginalis and Atopo-bium vaginae loads to predict bacterial vaginosis," Clinical Infectious Diseases, vol. 47, no. 1, pp. 33-43, 2008.
25. C. L. Haggerty, P. A. Totten, M. Ferris et al., "Clinical characteristics of bacterial vaginosis among women testing positive for fastidious bacteria," Sexually Transmitted Infections, vol. 85, no. 4, pp. 242-248, 2009.
26. J. Pépin, S. Deslandes, G. Giroux et al., "The complex vaginal fora of West African women with bacterial vaginosis," PLoS ONE, vol. 6, no. 9, Article ID e25082, 2011.
27. K. Fethers, J. Twin, C. K. Fairley et al., "Bacterial vaginosis (BV) candidate bacteria: associations with BV and behavioural practices in sexually-experienced and inexperienced women," PLoS ONE, vol. 7, no. 2, Article ID e30633, 2012.
28. S. Srinivasan, N. G. Hofman, M. T. Morgan et al., "Bacterial communities in women with bacterial vaginosis: high resolution phylogenetic analyses reveal relationships of microbiota to clinical criteria," PLoS ONE, vol. 7, no. 6, Article ID e37818, 2012.
29. E. Shipitsyna, A. Roos, R. Datcu et al., "Composition of the vaginal microbiota in women of reproductive age-sensitive and specifc molecular diagnosis of bacterial vaginosis is possible?" PLoS ONE, vol. 8, no. 4, Article ID e60670, 2013.
30. B. Foxman, A. Wen, U. Srinivasan et al., "Mycoplasma, bacterial vaginosis-associated bacteria BVAB3, race, and risk of preterm birth in a high-risk cohort," American Journal of Obstetrics & Gynecology, vol. 210, no. 3, pp. 226.e1-226.e7, 2014.
31. C. H. Livengood, "Bacterial vaginosis: an overview for 2009," Reviews in Obstetrics & Gynecology, vol. 2, no. 1, pp. 28-37, 2009.
32. D. N. Fredricks, T. L. Fiedler, and J. M. Marrazzo, "Molecular identifcation of bacteria associated with bacterial vaginosis," The New England Journal of Medicine, vol. 353, no. 18, pp. 1899-1911, 2005.
33. K. Mallard, K. Schopfer, and T. Bodmer, "Development of realtime PCR for the diferential detection and quantifcation of Ureaplasma urealyticum and Ureaplasma parvum," Journal of Microbiological Methods, vol. 60, no. 1, pp. 13-19, 2005.
34. D. N. Fredricks, T. L. Fiedler, K. K. Tomas, B. B. Oakley, and J. M. Marrazzo, "Targeted PCR for detection of vaginal bacteria associated with bacterial vaginosis," Journal of Clinical Microbiology, vol. 45, no. 10, pp. 3270-3276, 2007.
35. K. B. Waites, L. Xiao, V. Paralanov, R. M. Viscardi, and J. I. Glass, "Molecular methods for the detection of mycoplasma and ureaplasma infections in humans: a paper from the 2011 William Beaumont Hospital symposium on molecular pathology," Journal of Molecular Diagnostics, vol. 14, no. 5, pp. 437-450, 2012.
36. J. S. Huppert, J. R. Bates, A. F. Weber, N. Quinn, and C. A. Gaydos, "Abnormal vaginal pH and Mycoplasma genitalium infection," Journal of Pediatric & Adolescent Gynecology, vol. 26, no. 1, pp. 36-39, 2013.
37. A. Tiveljung, U. Forsum, and H.-J. Monstein, "Classifcation of the genus Mobiluncus based on comparative partial 16S rRNA gene analysis," International Journal of Systematic Bacteriology, vol. 46, no. 1, pp. 332-336, 1996.
38. D. E. Riley, M. Samadpour, and J. N. Krieger, "Detection of variable DNA repeats in diverse eukaryotic microorganisms by a single set of polymerase chain reaction primers," Journal of Clinical Microbiology, vol. 29, no. 12, pp. 2746-2751, 1991.
39. F. A. Nassar, F. A. Abu-Elamreen, M. E. Shubair, and F. A. Sharif, "Detection of Chlamydia trachomatis and Mycoplasma homi-nis, genitalium and Ureaplasma urealyticum by polymerase chain reaction in patients with sterile pyuria," Advance Medical Science, vol. 53, no. 1, pp. 80-86, 2008.
40. A. Ingianni, S. Petruzzelli, G. Morandotti, and R. Pompei, "Genotypic diferentiation of Gardnerella vaginalis by ampli-fed ribosomal DNA restriction analysis (ARDRA)," FEMS Immunology and Medical Microbiology, vol. 18, no. 1, pp. 61-66, 1997.
41. D. N. Fredricks, T. L. Fiedler, K. K. Tomas, C. M. Mitchell, and J. M. Marrazzo, "Changes in vaginal bacterial concentrations with intravaginal metronidazole therapy for bacterial vaginosis as assessed by quantitative PCR," Journal of Clinical Microbiology, vol. 47, no. 3, pp. 721-726, 2009.
42. M. Obata-Yasuoka, W. Ba-Tein, H. Hamada, and H. Hayashi, "A multiplex polymerase chain reaction-based diagnostic method for bacterial vaginosis," Obstetrics and Gynecology, vol. 100, no. 4, pp. 759-764, 2002.
43. M. J. Ferris, A. Masztal, and D. H. Martin, "Use of species-directed 16S rRNA gene PCR primers for detection of Atopo-bium vaginae in patients with bacterial vaginosis," Journal of Clinical Microbiology, vol. 42, no. 12, pp. 5892-5894, 2004.
44. C. M. Muvunyi, N. Dhont, R. Verhelst et al., "Evaluation of a new multiplex polymerase chain reaction assay STDFinder for the simultaneous detection of 7 sexually transmitted disease pathogens," Diagnostic Microbiology and Infectious Disease, vol. 71, no. 1, pp. 29-37, 2011.
45. C. J. McIver, N. Rismanto, C. Smith et al., "Multiplex PCR testing detection of higher-than-expected rates of cervical Mycoplasma, Ureaplasma, and Trichomonas and viral agent infections in sexually active australian women," Journal of Clinical Microbiology, vol. 47, no. 5, pp. 1358-1363, 2009.
46. D. N. Fredricks and D. A. Relman, "Application of polymerase chain reaction to the diagnosis of infectious diseases," Clinical Infectious Diseases, vol. 29, no. 3, pp. 475-488, 1999.
47. M. L. Mckechnie, R. Hillman, D. Couldwell et al., "Simultaneous identifcation of 14 genital microorganisms in urine by use of a multiplex PCR-based reverse line blot assay," Journal of Clinical Microbiology, vol. 47, no. 6, pp. 1871-1877, 2009.
48. R. P. Souza, A. L. P. de Abreu, É. C. Ferreira et al., "Simultaneous detection of seven sexually transmitted agents in human immunodefciency virus-infected Brazilian women by multiplex polymerase chain reaction," American Journal of Tropical Medicine and Hygiene, vol. 89, no. 6, pp. 1199-1202, 2013.
49. F. Gimenes, F. S. Medina, A. L. Abreu et al., "Sensitive simultaneous detection of seven sexually transmitted agents in semen by multiplex-PCR and of HPV by single PCR," PLoS ONE, vol. 9, no. 6, Article ID e98862, 2014.
50. A. Swidsinski, W. Mendling, V. Loening-Baucke et al., "An adherent Gardnerella vaginalis bioflm persists on the vaginal epithelium afer standard therapy with oral metronidazole," The American Journal of Obstetrics and Gynecology, vol. 198, no. 1, pp. 97.e 1-97.e6, 2008.
51. M. D. Harwich Jr., J. M. Alves, G. A. Buck et al., "Drawing the line between commensal and pathogenic Gardnerella vaginalis through genome analysis and virulence studies," BMC Genomics, vol. 11, no. 1, article 375, 2010.
52. Y. Turovskiy, K. Sutyak Noll, and M. L. Chikindas, "The aetiology of bacterial vaginosis," Journal of Applied Microbiology, vol. 110, no. 5, pp. 1105-1128, 2011.
53. J. Twin, C. S. Bradshaw, S. M. Garland, C. K. Fairley, K. Fethers, and S. N. Tabrizi, "The potential of metatranscriptomics for identifying screening targets for bacterial vaginosi," PLoS ONE, vol. 8, no. 9, Article ID e76892, 2013.