The complex vaginal flora of West African women with bacterial vaginosis

PLoS ONE

Volumn 6, Issue 9, 2011, Pages

  Pépin, Jacques ^a   Deslandes, Sylvie ^a   Giroux, Geneviève ^a   Sobéla, François ^a   Khonde, Nzambi ^a   Diakité, Soumaila ^b   Demeule, Sophie ^a   Labbé, Annie Claude ^c   Carrier, Nathalie ^d   Frost, Eric ^a  

^a UNIVERSITÉ DE SHERBROOKE   (Canada)

^b UNIVERSITÉ DE MONTRÉAL   (Canada)

^c MAISONNEUVE ROSEMONT HOSPITAL   (Canada)

^d CENTRE HOSPITALIER UNIVERSITAIRE DE SHERBROOKE   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CLOTRIMAZOLE; FLUCONAZOLE; METRONIDAZOLE; TINIDAZOLE;

ADULT; AFRICA; AGE; ANAEROCOCCUS; ARTICLE; ATOPOBIUM VAGINAE; BACTERIAL COUNT; BACTERIUM; BIFIDOBACTERIUM; DIALISTER; DISEASE ASSOCIATION; EGGERTHELLA; FEMALE; GARDNERELLA VAGINALIS; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS; LACTOBACILLUS; LEPTOTRICHIA; MAJOR CLINICAL STUDY; MEGASPHAERA ELSDENII; MOBILUNCUS; MYCOPLASMA HOMINIS; NONHUMAN; PENTONIPHILUS; PREVOTELLA; SEXUAL BEHAVIOR; SINGLE DRUG DOSE; VAGINA FLORA; VAGINITIS;

ADULT; AFRICA; BACTERIA; DNA, VIRAL; EPIDEMIOLOGIC STUDIES; FEMALE; HUMANS; POLYMERASE CHAIN REACTION; RANDOMIZED CONTROLLED TRIALS AS TOPIC; VAGINA; VAGINOSIS, BACTERIAL; YOUNG ADULT;

ANAEROCOCCUS; ATOPOBIUM VAGINAE; BACTERIA (MICROORGANISMS); BIFIDOBACTERIUM; DIALISTER; EGGERTHELLA; GARDNERELLA VAGINALIS; LACTOBACILLUS; LEPTOTRICHIA; MEGASPHAERA ELSDENII; MOBILUNCUS; MYCOPLASMA HOMINIS; PEPTONIPHILUS; PREVOTELLA;

EID: 80052918665     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0025082     Document Type: Article

References (46)

1. Eckert LO, (2006) Acute vulvovaginitis. N Engl J Med 355: 1244-1252.
2. Pepin J, Sobéla F, Khonde N, Agyarko-Poku T, Diakité S, et al. (2006) The syndromic management of vaginal discharge using single-dose treatments: a randomized controlled trial in West Africa. Bull World Health Organ 84: 729-738.
3. Hillier SL, Nugent RP, Eschenbach DA, Krohn MA, Gibbs RS, et al. (1995) Association between bacterial vaginosis and preterm delivery of a low-birth-weight infant. N Eng J Med 333: 1737-1742.
4. Moodley P, Connolly C, Sturm AW, (2002) Interrelationships among human immunodeficiency virus type 1 infection, bacterial vaginosis, trichomoniasis, and the presence of yeasts. J Infect Dis 185: 697-693.
5. Sewankambo N, Gray RH, Wawer MJ, Paxton L, McNaim D, et al. (1997) HIV-1 infection associated with abnormal vaginal flora morphology and bacterial vaginosis. Lancet 350: 546-550.
6. Taha TE, Hoover DR, Dallabetta GA, Kumwenda NI, Mtimavalye LA, et al. (1998) Bacterial vaginosis and disturbances of vaginal flora: association with increased acquisition of HIV. AIDS 12: 1699-1706.
7. Myer L, Denny L, Telerant R, de Souza M, Wright TC, et al. (2005) Bacterial vaginosis and susceptibility to HIV infection in South African women: a nested case-control study. J Infect Dis 192: 1372-1380.
8. Atashili J, Poole C, Ndumbe PM, Adimora AA, Smith JS, (2008) Bacterial vaginosis and HIV acquisition: a meta-analysis of published studies. AIDS 22: 1493-1501.
9. Sha BE, Zarrifard MR, Wang QJ, Chen HY, Bremer J, et al. (2005) Female genital-tract HIV load correlates inversely with Lactobacillus species but positively with bacterial vaginosis and Mycoplasma hominis. J Infect Dis 191: 25-32.
10. Thorsen P, Jensen IP, Jeune B, Ebbesen N, Arpi M, et al. (1998) Few microorganisms associated with bacterial vaginosis may constitute the pathologic core: a population-based microbiologic study among 3596 pregnant women. Am J Obstet Gynecol 178: 580-587.
11. Bradshaw CS, Tabrizi SN, Fairley CK, Morton AN, Rudland E, et al. (2006) The association of Atopobium vaginae and Gardnerella vaginalis with bacterial vaginosis and recurrence after oral metronidazole therapy. J Infect Dis 194: 8288-36.
12. Fredricks DN, Fiedler TL, Marrazzo JM, (2005) Molecular identification of bacteria associated with bacterial vaginosis. N Eng J Med 353: 1899-1911.
13. Verstraelen H, Verhelst R, Claeys G, Temmerman M, Vaneechoutte M, (2004) Culture-independent analysis of vaginal microflora: the unrecognized association of Atopobium vaginae with bacterial vaginosis. Am J Obstet Gynecol 191: 1130-1132.
14. Fredricks DN, Fiedler TL, Thomas KK, Oakley BB, Marazzo JM, (2007) Targeted PCR for detection of vaginal bacteria associated with bacterial vaginosis. J Clin Microbiol 45: 3270-3276.
15. Marrazzo JM, Thomas KK, Fiedler TL, Ringwood K, Fredricks DN, (2010) Risk for acquisition of bacterial vaginosis among women who report sex with women: a cohort study. PLoS One 5: e11139.
16. Marazzo JM, Thomas KK, Fiedler TL, Ringwood K, Fredricks DN, (2008) Relationship of specific vaginal bacteria and bacterial vaginosis treatment failure in women who have sex with women. Ann Intern Med 149: 20-28.
17. Haggerty CL, Totten PA, Ferris M, Martin DH, Hoferka S, et al. (2009) Clinical characteristics of bacterial vaginosis among women testing positive for fastidious bacteria. Sex Transm Dis 85: 242-248.
18. Rottingen JA, Cameron DW, Garnett GP, (2001) A systematic review for the epidemiologic interactions between classic sexually transmitted diseases and HIV. How much really is known? Sex Transm Dis 28: 579-597.
19. Dols JAM, Smit PW, Kort R, Reid GM, Schuren FHJ, et al. (2011) Microarray-based identification of clinically relevant vaginal bacteria in relation to bacterial vaginosis. Am J Obstet Gynecol 204: 305e1-e7.
20. Hummelen R, Fernandes AD, Macklaim JM, Dickson RJ, Changalucha J, et al. (2010) Deep sequencing of the vaginal microbiota of women with HIV. PLoS One 5: e12078.
21. Nugent RP, Krohn MA, Hillier SL, (1991) Reliability of diagnosing bacterial vaginosis is improved by a standardised method of Gram stain interpretation. J Clin Microbiol 29: 297-301.
22. Verhelst R, Verstraelen H, Claeys G, Verschraegen G, Delanghe J, et al. (2004) Cloning of 16S rRNA genes amplified from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis. BMC Microbiol 4: 16.
23. Matsuki T, Watanabe K, Fujimoto J, Miyamoto Y, Takada T, et al. (2002) Development of 16S rRNA-gene-targeted group-specific primers for the detection and identification of predominant bacteria in human feces. Appl Environ Microbiol 68: 5445-5451.
24. Zariffard MR, Saifuddin M, Sha BE, Spear GT, (2002) Detection of bacterial vaginosis-related organisms by real-time PCR for Lactobacilli, Gardnerella vaginalis and Mycoplasma hominis. FEMS Immunol Med Microbiol 34: 277-281.
25. Ferris MJ, Masztal A, Martin DH, (2004) Use of species-directed 16S rRNA gene PCR primers for detection of Atopobium vaginae in patients with bacterial vaginosis. J Clin Microbiol 42: 5892-5894.
26. Blanchard A, Yanez A, Dybvig K, Watson HL, Griffiths G, et al. (1993) Evaluation of intraspecies genetic variation within the 16S rRNA gene of Mycoplasma hominis and detection by polymerase chain reaction. J Clin Microbiol 31: 1358-1361.
27. Tiveljung A, Forsum U, Monstein HJ, (1996) Classification of the genus Mobiluncus based on comparative partial 16S rRNA gene analysis. Int J Syst Bacteriol 46: 332-336.
28. Verstraelen H, Verhelst R, Vaneechoutte M, Temmerman M, (2010) The epidemiology of bacterial vaginosis in relation to sexual behaviour. BMC Infect Dis 10: 81.
29. Bradshaw CS, Morton AN, Hocking J, Garland SM, Morris MB, et al. (2006) High recurrence rate of bacterial vaginosis over the course of 12 months after oral metronidazole therapy and factors associated with recurrence. J Infect Dis 193: 1478-1486.
30. Schwebke JR, Desmond R, (2005) Risk factors for bacterial vaginosis in women at high risk for sexually transmitted diseases. Sex Transm Dis 32: 654-658.
31. Morio F, Jean-Pierre H, Dubreuil L, Jumas-Bilak E, Calvet L, et al. (2007) Antimicrobial susceptibilities and clinical sources of Dialister species. Antimicrob Agents Chemother 51: 4498-4501.
32. Austin MN, Beigi RH, Meyn LA, Hillier SL, (2005) Microbiologic response to treatment of bacterial vaginosis with topical clindamycin or metronidazole. J Clin Microbiol 43: 4492-4497.
33. Liebetrau A, Rodloff AC, Behra-Miellet, Dubreuil L, (2003) In vitro activities of a new des-fluoro(6) quinolone, garenoxacin, against clinical anaerobic bacteria. Antimicrob Agents Chemother 47: 3667-3671.
34. Eribe ERK, Olsen I, (2008) Leptotrichia species in human infections. Anaerobe 14: 131-137.
35. Hillier SL, Lipinski C, Briselden AM, Eschenbach DA, (1993) Efficacy of intravaginal 0.75% metronidazole gel for the treatment of bacterial vaginosis. Obstet Gynecol 81: 963-967.
36. Fredricks DN, Fiedler TL, Thomas KK, Mitchell CM, Marazzo JM, (2009) Changes in vaginal bacterial concentrations with intravaginal metronidazole therapy for bacterial vaginosis as assessed by quantitative PCR. J Clin Microbiol 47: 721-726.
37. Mitchell CM, Hitti JE, Agnew KJ, Fredricks DN, (2009) Comparison of oral and vaginal metronidazole for treatment of bacterial vaginosis in pregnancy: impact on fastidious bacteria. BMC Infect Dis 9: 89.
38. Centers for Disease Control and Prevention (2006) Sexually Transmitted Diseases treatment guidelines 2006. Morb Mortal Wkly Rep 55 (RR-11): 1-64.
39. Beigi RH, Austin MN, Meyn LA, Krohn MA, Hillier SL, (2004) Antimicrobial resistance associated with the treatment of bacterial vaginosis. Am J Obstet Gynecol 191: 1124-1129.
40. Oduyebo OO, Anorlu RI, Ogunsola FT, (2009) The effects of antimicrobial therapy on bacterial vaginosis in non-pregnant women. Cochrane Database of Systematic Reviews 3: CD006055.
41. Swidsinski A, Mendling W, Loening-Baucke V, Swidsinski S, Dorffel Y, et al. (2008) An adherent Gardnerella vaginalis biofilm persists on the vaginal epidetlium after standard therapy with oral metronidazole. Am J Obstet Gynecol 198: e1-97.e6.
42. Oakley BB, Fiedler TL, Marazzo JM, Fredricks DN, (2008) Diversity of human vaginal communities and associations with clinically defined bacterial vaginosis. Appl Environ Microbiol 74: 4898-4909.
43. Ling Z, Kong J, Liu F, Zhu H, Chen X, et al. (2010) Molecular analysis of the diversity of vaginal microbiota associated with bacterial vaginosis. BMC Genomics 11: 488.
44. Koumans EH, Sternberg M, Bruce C, McQuillan G, Kendrick J, et al. (2007) The prevalence of bacterial vaginosis in the United States, 2001-2004; associations with symptoms, sexual behaviors, and reproductive health. Sex Transm Dis 34: 864-869.
45. Ravel J, Gajer P, Abdo Z, Schneider GM, Sara S, et al. (2010) Vaginal microbiome of reproductive-age women. Proc Natl Acad Sci U S A doi:10.1073/pnas.10002611107.
46. Biagi E, Vitali B, Pugliese C, Candela M, Donders GGG, Brigidi P, (2009) Quantitative variations in the vaginal bacterial population associated with asymptomatic infections: a real-time polymerase chain reaction study. Eur J Clin Microbiol Infect Dis 28: 281-285.