An improved protocol and a new grinding device for extraction of genomic DNA from microorganisms by a two-step extraction procedure

Genetics and Molecular Research

Volumn 11, Issue 2, 2012, Pages 1532-1543

  Zhang, S S ^a   Chen, D ^a   Lu, Q ^a  

^a NATIONAL ENGINEERING RESEARCH CENTER FOR NON FOOD BIOREFINERY   (China)

Author keywords

DNA extraction; Electric mini grinder; Silica sand

Indexed keywords

BUFFER; GENOMIC DNA; SILICON DIOXIDE;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL SPORE; CELL LYSATE; CONTROLLED STUDY; DNA DETERMINATION; DNA EXTRACTION; DNA STRUCTURE; ELECTRICAL EQUIPMENT; ESCHERICHIA COLI; GRINDING; LIMIT OF DETECTION; NONHUMAN; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; TRICHODERMA VIRIDE; TWO STAGE EXTRACTION;

EID: 84930476368     PISSN: None     EISSN: 16765680     Source Type: Journal    
DOI: 10.4238/2012.May.21.10     Document Type: Article

References (24)

1. Al-Samarrai TH and Schmid J (2000). A simple method for extraction of fungal genomic DNA. Lett. Appl. Microbiol. 30: 53-56.
2. Astegiano S, Sidoti F, Costa C, Ostorero A, et al. (2010). Human cytomegalovirus load in fresh and glycerolized skin grafts. New Microbiol. 33: 253-256.
3. Chi MH, Park SY and Lee YH (2009). A quick and safe method for fungal DNA extraction. Plant Pathol. J. 25: 108-111.
4. Clark-Curtiss JE, Jacobs WR, Docherty MA, Ritchie LR, et al. (1985). Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae. J. Bacteriol. 161: 1093-1102.
5. Deden H, Deufel A, Eisel D and Grünewald-Janho S (2006). PCR Applications Manual. 3rd edn. Roche Diagnostics GmbH, Mannheim.
6. Fredricks DN and Relman DA (1998). Improved amplification of microbial DNA from blood cultures by removal of the PCR inhibitor sodium polyanetholesulfonate. J. Clin. Microbiol. 36: 2810-2816.
7. Griffiths LJ, Anyim M, Doffman SR, Wilks M, et al. (2006). Comparison of DNA extraction methods for Aspergillus fumigatus using real-time PCR. J. Med. Microbiol. 55: 1187-1191.
8. Harju S, Fedosyuk H and Peterson KR (2004). Rapid isolation of yeast genomic DNA: Bust n' Grab. BMC Biotechnol. 4: 8.
9. Karakousis A, Tan L, Ellis D, Alexiou H, et al. (2006). An assessment of the efficiency of fungal DNA extraction methods for maximizing the detection of medically important fungi using PCR. J. Microbiol. Methods 65: 38-48.
10. Kuske CR, Banton KL, Adorada DL, Stark PC, et al. (1998). Small-scale DNA sample preparation method for field PCR detection of microbial cells and spores in soil. Appl. Environ. Microbiol. 64: 2463-2472.
11. Liu KH, Yeh YL and Shen WC (2011). Fast preparation of fungal DNA for PCR screening. J. Microbiol. Methods 85: 170-172.
12. Maarit NR, Heiskanen I, Wallenius K and Lindstrom K (2001). Extraction and purification of DNA in rhizosphere soil samples for PCR-DGGE analysis of bacterial consortia. J. Microbiol. Methods 45: 155-165.
13. Melo SC, Pungartnik C, Cascardo JC and Brendel M (2006). Rapid and efficient protocol for DNA extraction and molecular identification of the basidiomycete Crinipellis perniciosa. Genet. Mol. Res. 5: 851-855.
14. Moslem MA, Bahkali AH, Abd-Elsalam KA and Wit PJ (2010). An efficient method for DNA extraction from Cladosporioid fungi. Genet. Mol. Res. 9: 2283-2291.
15. Müller FM, Werner KE, Kasai M, Francesconi A, et al. (1998). Rapid extraction of genomic DNA from medically important yeasts and filamentous fungi by high-speed cell disruption. J. Clin. Microbiol. 36: 1625-1629.
16. Niwa T, Kawamura Y, Katagiri Y and Ezaki T (2005). Lytic enzyme, labiase for a broad range of Gram-positive bacteria and its application to analyze functional DNA/RNA. J. Microbiol. Methods 61: 251-260.
17. Okada H, Tada K, Sekiya T, Yokoyama K, et al. (1998). Molecular characterization and heterologous expression of the gene encoding a low-molecular-mass endoglucanase from Trichoderma reesei QM9414. Appl. Environ. Microbiol. 64: 555-563.
18. Rajagopalan G and Krishnan C (2008). Alpha-amylase production from catabolite derepressed Bacillus subtilis KCC103 utilizing sugarcane bagasse hydrolysate. Bioresour. Technol. 99: 3044-3050.
19. Sambrook J and Russell DW (2001). Molecular Cloning: A Laboratory Manual. 3rd edn. Cold Spring Harbor, New York.
20. Tendulkar SR, Gupta A and Chattoo BB (2003). A simple protocol for isolation of fungal DNA. Biotechnol. Lett. 25: 1941-1944.
21. Tong AH and Boone C (2006). Synthetic genetic array analysis in Saccharomyces cerevisiae. Methods Mol. Biol. 313: 171-192.
22. Waites MJ, Morgan NL, Rockey JS and Higton G (2001). Industrial Microbiology: An Introduction. Blackwell Science, London, 16-34.
23. Yamamoto Y, Kohno S, Koga H, Kakeya H, et al. (1995). Random amplified polymorphic DNA analysis of clinically and environmentally isolated Cryptococcus neoformans in Nagasaki. J. Clin. Microbiol. 33: 3328-3332.
24. Zhang YJ, Zhang S, Liu XZ, Wen HA, et al. (2010). A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains. Lett. Appl. Microbiol. 51: 114-118.