A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains

Letters in Applied Microbiology

Volumn 51, Issue 1, 2010, Pages 114-118

  Zhang, Y J ^a,b   Zhang, S ^a   Liu, X Z ^b   Wen, H A ^b   Wang, M ^c  

^a SHANXI UNIVERSITY   (China)

^b INSTITUTE OF MICROBIOLOGY   (China)

^c Tibet Agriculture and Animal Husbandry University   (China)

Author keywords

DNA extraction; Fungi; Internal transcribed spacer (ITS) region; Ophiocordyceps sinensis; PCR amplification; Thermolysis

Indexed keywords

AMPLIFICATION; DNA; EXTRACTION; FUNGI; TRANSCRIPTION;

BREAK DOWN; CELL WALLS; COPY NUMBER; DNA EXTRACTION; DNA SAMPLES; FUNGAL ISOLATES; FUNGAL SPECIES; FUNGAL STRAINS; GENOMIC DNA; GENOMIC DNA EXTRACTION; INTERNAL TRANSCRIBED SPACERS; LYSIS BUFFER; MOLECULAR ASSAYS; OPHIOCORDYCEPS SINENSIS; PCR AMPLIFICATION; PURE WATER; RAPID METHOD; REGIONS OF INTEREST; SIMPLE METHOD; STARTING MATERIALS; YEAST CELL;

GENES;

GENOMIC DNA; FUNGAL DNA;

EXTRACTION METHOD; FUNGUS; GENETIC ANALYSIS; HEATING; LYSIS; POLYMERASE CHAIN REACTION; THERMAL DECOMPOSITION;

ACREMONIUM; ARTICLE; ASPERGILLUS; BEAUVERIA; CADOPHORA; CHRYSOSPORIUM; CLADOSPORIUM; CYLINDROCARPON; DNA EXTRACTION; EPICOCCUM; FIMETARIELLA; FUNGAL STRAIN; FUNGUS; FUNGUS IDENTIFICATION; FUSARIUM; GLIOCLADIUM; GYMNOASCUS; LEOHUMICOLA; MORTIERELLA; MUCOR; MYCELIUM; NEONECTRIA; NONHUMAN; OPHIOCORDYCEPS; PAECILOMYCES; PENICILLIUM; STILBELLA; THERMOLYSIS METHOD; TOLYPOCLADIUM; TRICHOCLADIUM; TRICHODERMA; TRICLADIUM; TUMULARIA; YEAST; YEAST CELL; ZALERION; GENETICS; GENOMICS; ISOLATION AND PURIFICATION; MOLECULAR BIOLOGY; MYCOLOGY; PROCEDURES;

FUNGI;

DNA, FUNGAL; FUNGI; GENOMICS; MOLECULAR BIOLOGY; MYCOLOGY;

EID: 77953482272     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2010.02867.x     Document Type: Article

References (22)

1. Ashktorab, H. Cohen, R.J. (1992) Facile isolation of genomic DNA from filamentous fungi. BioTechniques 13, 198 200.
2. Dighton, J., White, J.F. Oudemans, P. (2005) The Fungal Community: Its Organization and Role in the Ecosystem.
3. Doyle, J.J. Doyle, J.L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19, 11 15.
4. Faggi, E., Pini, G. Campisi, E. (2005) Use of magnetic beads to extract fungal DNA. Mycoses 48, 3 7.
5. Goodwin, D.C. Lee, S.B. (1993) Rapid, microwave mini-prep of total genomic DNA from fungi, plants, protists and animals for PCR. BioTechniques 15, 438 444.
6. Griffin, D.W., Kellogg, C.A., Peak, K.K. Shinn, E.A. (2002) A rapid and efficient assay for extracting DNA from fungi. Lett Appl Microbiol 34, 210 214.
7. Hibbett, D.S., Binder, M., Bischoff, J.F., Blackwell, M., Cannon, P.F., Eriksson, O.E., Huhndorf, S., James, T. et al. (2007) A higher-level phylogenetic classification of the Fungi. Mycol Res 111, 509 547.
8. Kwok, S. Higuchi, R. (1989) Avoiding false positives with PCR. Nature 339, 237 238.
9. Lee, S.B., Milgroom, M.G. Taylor, J.W. (1988) A rapid, high yield mini-prep method for isolation of total genomic DNA from fungi. Fungal Genet Newsl 35, 23 24.
10. Li, S.L., Zhou, B., Yang, L.Y., Li, Z.Y., Zhang, Q. Chen, Y.W. (2002) An improved method for extracting fungal DNA. J Yunnan Univ 24, 471 472.
11. Liu, X.J., Guo, Y.L., Yu, Y.X. Zeng, W. (1989) Isolation and identification of the anamorphic state of Cordyceps sinensis (Berk.) Sacc. Acta Mycol Sin 8, 35 40.
12. Min, J., Arganoza, M.T., Ohrnberger, J., Xu, C. Akins, R.A. (1995) Alternative methods of preparing whole-cell DNA from fungi for dot-blot, restriction analysis, and colony filter hybridization. Anal Biochem 225, 94 100.
13. Paterson, R.R.M. (2007) Internal amplification controls have not been employed in fungal PCR hence potential false negative results. J Appl Microbiol 102, 1 10.
14. Paterson, R.R.M. (2008) Fungal enzyme inhibitors as pharmaceuticals, toxins, and scourge of PCR. Curr Enzyme Inhib 4, 46 59.
15. Porebski, S., Bailey, L. Baum, B. (1997) Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Rep 15, 8 15.
16. Tendulkar, S.R., Gupta, A. Chattoo, B.B. (2003) A simple protocol for isolation of fungal DNA. Biotechnol Lett 25, 1941 1944.
17. Wang, M., Sun, M.H., Zhang, Y.J., Luo, Z. Zhuo, G. (2006) Preliminary studies on microflora of Cordyceps sinensis in Tibet. Edible fungi China 25 (Suppl. 6 8.
18. Wu, Z.H., Wang, T.H., Huang, W. Qu, Y.B. (2001) A simplified method for chromosome DNA preparation from filamentous Fungi. Mycosystema 20, 575 577.
19. Xue, S.J., Yue, T.L., Guan, J., Yuan, Y.H. Gao, Z.P. (2006) An improved method for extracting fungal DNA. Food Res Develop 27, 39 40.
20. Zhang, Y.H., Wei, D.S., Xing, L.J. Li, M.C. (2008a) A modified method for isolating DNA from fungus. Microbiology China 35, 466 469.
21. Zhang, Y.J., Liu, X.Z. Wang, M. (2008b) Cloning, expression, and characterization of two novel cuticle-degrading serine proteases from the entomopathogenic fungus Cordyceps sinensis. Res Microbiol 159, 462 469.
22. Zhang, Y.J., Xu, L.L., Zhang, S., Liu, X.Z., An, Z.Q., Wang, M. Guo, Y.L. (2009) Genetic diversity of Ophiocordyceps sinensis, a medicinal fungus endemic to the Tibetan Plateau: Implications for its evolution and conservation. BMC Evol Biol 9, 290.