Measurement of glycolysis reactants by high-throughput solid phase extraction with tandem mass spectrometry: Characterization of pyrophosphate-dependent phosphofructokinase as a case study

Analytical Biochemistry

Volumn 482, Issue , 2015, Pages 40-47

  Rye, Peter T ^a   LaMarr, William A ^a  

^a Agilent Technologies   (United States)

Author keywords

Glycolysis; High throughput; Mass spectrometry; Phosphofructokinase; RapidFire; Solid phase extraction

Indexed keywords

AUTOMATION; MASS SPECTROMETRY; METABOLISM; PATHOLOGY; PHASE SEPARATION;

GLYCOLYSIS; HIGH THROUGHPUT; PHOSPHOFRUCTOKINASE; RAPIDFIRE; SOLID-PHASE EXTRACTION;

EXTRACTION;

6 PHOSPHOFRUCTOKINASE; PYROPHOSPHATE; PYROPHOSPHATE DEPENDENT PHOSPHOFRUCTOKINASE; UNCLASSIFIED DRUG; PHOSPHOTRANSFERASE; PYROPHOSPHATE-FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASE;

ACTIVATION CONSTANT; ARTICLE; AUTOMATION; BINDING SITE; CONCENTRATION RESPONSE; ENZYME ANALYSIS; ENZYME BINDING; GLYCOLYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; IN VITRO STUDY; LIMIT OF DETECTION; MICHAELIS CONSTANT; MICHAELIS MENTEN KINETICS; PRIORITY JOURNAL; SOLID PHASE EXTRACTION; TANDEM MASS SPECTROMETRY; ENZYMOLOGY; HUMAN; METABOLISM; METABOLOMICS; POTATO; PROCEDURES;

GLYCOLYSIS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; METABOLOMICS; PHOSPHOTRANSFERASES; SOLANUM TUBEROSUM; SOLID PHASE EXTRACTION; TANDEM MASS SPECTROMETRY;

EID: 84930274369     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2015.03.029     Document Type: Article

References (38)

1. H. Pelicano, D. Martin, R. Xu, and P. Huang Glycolysis inhibition for anticancer treatment Oncogene 25 2006 4633 4646
2. P. Porporato, S. Dhup, R. Dadhich, T. Copetti, and P. Sonveaux Anticancer targets in the glycolytic metabolism of tumors: a comprehensive review Front. Pharmacol. 2 2011 http://dx.doi.org/10.3389/fphar.2011.00049
3. B. Clem, S. Telang, A. Clem, A. Yalcin, J. Meier, A. Simmons, M. Rasku, S. Arumugam, W. Dean, J. Eaton, A. Lane, J. Trent, and J. Chesney Small-molecule inhibition of 6-phosphofructo-2-kinase activity suppresses glycolytic flux and tumor growth Mol. Cancer Ther. 7 2008 110 120
4. J. Huck, E. Struys, N. Verhoeven, C. Jakobs, and M. van der Knapp Profiling of pentose phosphate pathway intermediates in blood spots by tandem mass spectrometry: application to transaldolase deficiency Clin. Chem. 49 2003 1375 1380
5. J. Zhang, T. Chung, and K. Oldenburg A simple statistical parameter for use in evaluation and validation of high throughput screening assays J. Biomol. Screen. 4 1999 67 73
6. A. Zhu, R. Romero, and H. Petty An enzymatic fluorimetric assay for glucose-6-phosphate: application in an in vitro Warburg-like effect Anal. Biochem. 388 2009 97 101
7. E. Van Schaftingen, B. Lederer, R. Bartrons, and H. Hers A kinetic study of pyrophosphate:fructose-6-phosphate phosphotransferase from potato tubers: Application to a microassay of fructose 2,6-bisphosphate Eur. J. Biochem. 129 1982 191 195
8. Y. Gibon, H. Vigeolas, A. Tiessen, P. Geigenberger, and M. Stitt Sensitive and high throughput metabolite assays for inorganic pyrophosphate, ADPGlc, nucleotide phosphates, and glycolytic intermediates based on a novel enzymatic cycling system Plant J. 30 2002 221 235
9. G. Michal H. Bergmeyer, Methods of Enzymatic Analysis, vol. 6: Metabolites 1: Carbohydrates 1983 Verlag Chemie Weinheim, Germany 191 199 (Chapter 3)
10. D. De Jong, and W. Woodlief Fluorometric assay of tobacco leaf dehydrogenases with resazurin Biochim. Biophys. Acta 484 1977 249 259
11. A. Zhu, R. Romero, and H. Petty An enzymatic colorimetric assay for glucose-6-phosphate Anal. Biochem. 419 2011 266 270
12. J. van Dam, M. Eman, J. Frank, H. Lange, G. van Dedem, and S. Heijnen Analysis of glycolytic intermediates in Saccharomyces cerevisiae using anion exchange chromatography and electrospray ionization with tandem mass spectrometric detection Anal. Chim. Acta 460 2002 209 218
13. Y. Sekiguchi, N. Mitsuhashi, T. Kokaji, H. Miyakoda, and T. Mimura Development of a comprehensive analytical method for phosphate metabolites in plants by ion chromatography coupled with tandem mass spectrometry J. Chromatogr. 1085 2005 131 136
14. C. Antonio, T. Larson, A. Gilday, I. Graham, E. Bergstrom, and J. Thomas-Oates Quantification of sugars and sugar phosphates in Arabidopsis thaliana tissues using porous graphitic carbon liquid chromatography-electrospray ionization mass spectrometry J. Chromatogr. 1172 2007 170 178
15. T. Nielsen Fructose-1,6-bisphosphate is an allosteric activator of pyrophosphate:fructose-6-phosphate 1-phosphotransferase Plant Physiol. 108 1995 69 73
16. T. Schirmer, and P. Evans Structural basis of the allosteric behavior of phosphofructokinase Nature 343 1990 140 145
17. N. Kruger, and D. Dennis Molecular properties of pyrophosphate:fructose-6-phosphate phosphotransferase from potato tuber Arch. Biochem. Biophys. 256 1987 273 279
18. M. Stitt Fructose-2,6-bisphosphate as a regulatory molecule in plants Annu. Rev. Plant Physiol. Plant Mol. Biol. 41 1990 153 185
19. M. Stitt Product inhibition of potato tuber pyrophosphate:fructose-6-phosphate phosphotransferase by phosphate and pyrophosphate Plant Physiol. 89 1989 628 633
20. F. Podesta, and W. Plaxton Fluorescence study of ligand binding to potato tuber pyrophosphate-dependent phosphofructokinase: evidence for competitive binding between fructose-1,6-bisphosphate and fructose-2,6-bisphosphate Arch. Biochem. Biophys. 414 2003 101 107
21. T. Nielsen, and B. Wischmann Quantitative aspects of the in vivo regulation of pyrophosphate:fructose-6-phosphate 1-phosphotransferase by fructose-2,6-bisphosphate Plant Physiol. 109 1995 1033 1038
22. M. Stitt, and A. Vasella Biological action of phosphonate analogs of fructose 2,6-bisphosphonate on enzymes from higher plants FEBS Lett. 228 1987 60 64
23. B. Bertagnolli, E. Younathan, R. Voll, and P. Cook Kinetic studies on the activation of pyrophosphate-dependent phosphofructokinase from mung bean by fructose 2,6-bisphosphate and related compounds Biochemistry 25 1986 4682 4687
24. A. Balogh, J. Wong, C. Wotzel, J. Soll, C. Cseke, and B. Buchanan Metabolite-mediated catalyst conversion of PFK and PFP: a mechanism of enzyme regulation in green plants FEBS Lett. 169 1984 287 292
25. J. Wong, A. Balogh, and B. Buchanan Pyrophosphate functions as phosphoryl donor with UDP-glucose-treated mammalian phosphofructokinase Biochem. Biophys. Res. Commun. 121 1984 842 847
26. N. Kruger, and D. Dennis A source of apparent pyrophosphate: fructose 6-phosphate phosphotransferase activity in rabbit muscle phosphofructokinase Biochem. Biophys. Res. Commun. 126 1985 320 326
27. N. Kruger, and D. Dennis Reassessment of an apparent hyperactive form of phosphofructokinase from plants Plant Physiol. 78 1985 645 648
28. M. Bujara, M. Schumperli, R. Pellaux, M. Heinemann, and S. Panke Optimization of a blueprint for in vitro glycolysis by metabolic real-time analysis Nat. Chem. Biol. 5 2011 271 277
29. T. Holt, B. Choi, N. Geoghagen, K. Jensen, Q. Luo, W. LaMarr, G. Makara, L. Malkowitz, C. Ozbal, Y. Xiong, C. Dufresne, and M. Luo Label-free high-throughput screening via mass spectrometry: a single cystathionine quantitative method for multiple applications Assay Drug Dev. Technol. 5 2009 495 506
30. E. Langsdorf, A. Malikzay, W. Lamarr, D. Daubaras, C. Kravec, R. Zhang, R. Hart, F. Monsma, T. Black, C. Ozbal, L. Miesel, and C. Lunn Screening for antibacterial inhibitors of the UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase (LpxC) using a high-throughput mass spectrometry assay J. Biomol. Screen. 1 2010 52 61
31. M. Highkin, M. Yates, O. Nemirovskiy, W. Lamarr, G. Munie, J. Rains, J. Masferrer, and M. Nagiec High-throughput screening assay for sphingosine kinase inhibitors in whole blood using RapidFire mass spectrometry J. Biomol. Screen. 2 2011 272 277
32. V. Miller SPE-MS analysis of absorption, distribution, metabolism, and excretion assays: a tool to increase throughput and steamline workflow Bioanalysis 9 2012 1111 1121
33. M. Leveridge, A. Bardera, W. LaMarr, A. Billinton, B. Bellenie, C. Edge, P. Francis, E. Christodoulou, A. Shillings, M. Hibbs, A. Fosberry, R. Tanner, P. Hardwicke, P. Craggs, Y. Sinha, O. Elegbe, E. Alvarez-Ruiz, J. Martin-Plaza, V. Barroso-Poveda, S. Baddeley, C. Chung, and J. Hutchinson Lead discovery for microsomal prostaglandin E synthase using a combination of high-throughput fluorescent-based assays and RapidFire mass spectrometry J. Biomol. Screen. 5 2012 641 650
34. E. Vanderporten, L. Frick, R. Turincio, P. Thana, W. Lamarr, and Y. Liu Label-free high-throughput assays to screen and characterize novel lactate dehydrogenase inhibitors Anal. Biochem. 2 2013 115 122
35. P. Rye, L. Frick, C. Ozbal, and W. Lamarr Advances in label-free screening approaches for studying sirtuin-mediated deacetylation J. Biomol. Screen. 10 2011 1217 1226
36. M. Plant, T. Dineen, A. Cheng, A. Long, H. Chen, and K. Morgenstern Screening for lysine-specific demethylase-1 inhibitors using a label-free high-throughput mass spectrometry assay Anal. Biochem. 2 2011 217 227
37. S. Hutchinson, M. Leveridge, M. Heathcote, P. Francis, L. Williams, M. Gee, J. Munoz-Muriedas, B. Leavens, A. Shillings, E. Jones, P. Homes, S. Baddeley, C. Chung, A. Bridges, and A. Argyrou Enabling lead discovery for histone lysine demethylases by high-throughput RapidFire mass spectrometry J. Biomol. Screen. 1 2012 39 48
38. P. Rye, L. Frick, C. Ozbal, and W. Lamarr Advances in label-free screening approaches for studying histone acetyltransferases J. Biomol. Screen. 10 2011 1186 1195