96-Well Format-Based Microfluidic Platform for Parallel Interconnection of Multiple Multicellular Spheroids

Journal of Laboratory Automation

Volumn 20, Issue 3, 2015, Pages 274-282

  Kim, Jin Young ^a   Fluri, David A ^b   Kelm, Jens M ^b   Hierlemann, Andreas ^a   Frey, Olivier ^a  

^a ETH ZURICH   (Switzerland)

^b InSphero AG   (Switzerland)

Author keywords

automated biology; high throughput screening; lab on a chip; microfluidics; microtechnology

Indexed keywords

DIAGNOSIS; HISTOLOGY; INTEGRATED CIRCUIT INTERCONNECTS; LAB-ON-A-CHIP; TUMORS;

5-FLUOROURACIL; CONTINUOUS MEDIA; HIGH THROUGHPUT SCREENING; MICROFLUIDIC CHANNEL; MICROFLUIDIC PLATFORMS; MICROTECHNOLOGY; MULTICELLULAR SPHEROID; PARALLEL INTERCONNECTION;

MICROFLUIDICS;

FLUOROURACIL;

ANIMAL TISSUE; ARTICLE; CELL PROLIFERATION; CELL VIABILITY; FLUORESCENCE IMAGING; FLUORESCENCE MICROSCOPY; GRAVITY; HYDRODYNAMICS; METABOLIC ACTIVITY ASSAY; MICROFLUIDICS; MICROSCOPY; MICROTECHNOLOGY; NONHUMAN; RAT; SPHEROID CELL; TUMOR SPHEROID; ANIMAL; CELL CULTURE TECHNIQUE; CYTOLOGY; DRUG EFFECTS; HCT116 CELL LINE; HUMAN; LAB ON A CHIP; LIVER; MULTICELLULAR SPHEROID; PRIMARY CELL CULTURE; PROCEDURES; UTILIZATION;

ANIMALS; CELL CULTURE TECHNIQUES; FLUOROURACIL; HCT116 CELLS; HUMANS; LAB-ON-A-CHIP DEVICES; LIVER; MICROFLUIDICS; PRIMARY CELL CULTURE; RATS; SPHEROIDS, CELLULAR;

EID: 84930249306     PISSN: 22110682     EISSN: 22110690     Source Type: Journal    
DOI: 10.1177/2211068214564056     Document Type: Article

References (24)

1. Abbott A.Biology’s New Dimension.Nature. 2003;424:870-872
2. Pampaloni F.,Reynaud E. G.,Stelzer E. H. K.The Third Dimension Bridges the Gap between Cell Culture and Live Tissue.Nat. Rev. Mol. Cell Biol. 2007;8:839-845
3. Friedrich J.,Ebner R.,Kunz-Schughart L. A.Experimental Anti-Tumor Therapy in 3-D: Spheroids—Old Hat or New Challenge?.Int. J. Radiat. Biol. 2007;83:849-871
4. Hirschhaeuser F.,Menne H.,Dittfeld C., et al.Multicellular Tumor Spheroids: An Underestimated Tool Is Catching up Again.J. Biotechnol. 2010;148:3-15
5. Drewitz M.,Helbling M.,Fried N., et al.Towards Automated Production and Drug Sensitivity Testing Using Scaffold-Free Spherical Tumor Microtissues.Biotechnol. J. 2011;6:1488-1496
6. Messner S.,Agarkova I.,Moritz W., et al.Multi-Cell Type Human Liver Microtissues for Hepatotoxicity Testing.Arch. Toxicol. 2013;87:209-213
7. Hsiao A. Y.,Tung Y.-C.,Qu X., et al.384 Hanging Drop Arrays Give Excellent Z-Factors and Allow Versatile Formation of Co-Culture Spheroids.Biotechnol. Bioeng. 2012;109:1293-1304
8. Kovarik M. L.,Gach P. C.,Ornoff D. M., et al.Micro Total Analysis Systems for Cell Biology and Biochemical Assays.Anal. Chem. 2012;84:516-540
9. Wagner I.,Materne E.-M.,Brincker S., et al.A Dynamic Multi-Organ-Chip for Long-Term Cultivation and Substance Testing Proven by 3D Human Liver and Skin Tissue Co-Culture.Lab Chip. 2013;13:3538-3547
10. Huh D.,Torisawa Y.,Hamilton G. A., et al.Microengineered Physiological Biomimicry: Organs-on-Chips.Lab Chip. 2012;12:2156-2164
11. Sonntag F.,Schilling N.,Mader K., et al.Design and Prototyping of a Chip-Based Multi-Micro-Organoid Culture System for Substance Testing, Predictive to Human (Substance) Exposure.J. Biotechnol. 2010;148:70-75
12. Sung J. H.,Shuler M. L.A Micro Cell Culture Analog (MicroCCA) with 3-D Hydrogel Culture of Multiple Cell Lines to Assess Metabolism-Dependent Cytotoxicity of Anti-Cancer Drugs.Lab Chip. 2009;9:1385-1394
13. Imura Y.,Sato K.,Yoshimura E.Micro Total Bioassay System for Ingested Substances: Assessment of Intestinal Absorption, Hepatic Metabolism, and Bioactivity.Anal. Chem. 2010;82:9983-9988
14. Khademhosseini A.,Langer R.,Borenstein J., et al.Microscale Technologies for Tissue Engineering and Biology.Proc. Natl. Acad. Sci. U. S. A. 2006;103:2480-2487
15. Torisawa Y.,Takagi A.,Nashimoto Y., et al.A Multicellular Spheroid Array to Realize Spheroid Formation, Culture, and Viability Assay on a Chip.Biomaterials. 2007;28:559-566
16. Wu L. Y.,Di Carlo D.,Lee L. P.Microfluidic Self-Assembly of Tumor Spheroids for Anticancer Drug Discovery.Biomed. Microdevices. 2008;10:197-202
17. Hsiao A. Y.,Torisawa Y.,Tung Y.-C., et al.Microfluidic System for Formation of PC-3 Prostate Cancer Co-Culture Spheroids.Biomaterials. 2009;30:3020-3027
18. Ruppen J.,Cortes-Dericks L.,Marconi E., et al.A Microfluidic Platform for Chemoresistive Testing of Multicellular Pleural Cancer Spheroids.Lab Chip. 2014;14:1198-1205
19. Trietsch S. J.,Israëls G. D.,Joore J., et al.Microfluidic Titer Plate for Stratified 3D Cell Culture.Lab Chip. 2013;13:3548-3554
20. Lee P. J.,Ghorashian N.,Gaige T. A., et al.Microfluidic System for Automated Cell-Based Assays.JALA Charlottesv. Va. 2007;12:363-367
21. Chang T.,Mikheev A. M.,Monnat R. J., et al.A 96-Well, Plate-Basel Microfluidic Device for Multiplexed Chemosensitivity Testing in Intact Tissues.17th Int. Conference on Miniaturized Systems for Chemistry and Life Science (microTAS); 2013Freiburg, Germany; 2013. 506.
22. Frey O.,Mohanty S.,Moritz W., et al.Towards a “Body on a Chip” Using Spherical Microtissues in a Microfluidic Network.16th Int. Conference on Miniaturized Systems for Chemistry and Life Science (microTAS); 2012Okinawa, Japan; 2012. 1540.
23. Ishihara K.,Ziats N. P.,Tierney B. P., et al.Protein Adsorption from Human Plasma Is Reduced on Phospholipid Polymers.J. Biomed. Mater. Res. 1991;25:1397-1407
24. Van Midwoud P. M.,Janse A.,Merema M. T., et al.Comparison of Biocompatibility and Adsorption Properties of Different Plastics for Advanced Microfluidic Cell and Tissue Culture Models.Anal. Chem. 2012;84:3938-3944