High-resolution whole-brain staining for electron microscopic circuit reconstruction

Nature Methods

Volumn 12, Issue 6, 2015, Pages 541-546

  Mikula, Shawn ^a,b   Denk, Winfried ^a,b  

^a MAX PLANCK INSTITUTE FOR MEDICAL RESEARCH   (Germany)

^b MAX PLANCK INSTITUTE OF NEUROBIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

OSMIUM; PYROGALLOL;

ANIMAL TISSUE; ARTICLE; BRAIN WIDE REDUCED OSMIUM STAINING WITH PYROGALLOL MEDIATED AMPLIFICATION; CELL EXPANSION; CONNECTOME; ELECTRIC FIELD; ELECTRON MICROSCOPY; EXTRACELLULAR SPACE; LIPID MEMBRANE; MOUSE; MYELIN SHEATH; NONHUMAN; PRECIPITATION; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; SOMATOSENSORY CORTEX; STAINING; SYNAPSE; ULTRASTRUCTURE; ANIMAL; BRAIN; BRAIN MAPPING; ELECTROPHYSIOLOGY; HUMAN; NEURITE; PHYSIOLOGY; PROCEDURES;

ANIMALS; BRAIN; BRAIN MAPPING; ELECTROPHYSIOLOGICAL PHENOMENA; HUMANS; MICE; MICROSCOPY, ELECTRON; NEURITES; STAINING AND LABELING; SYNAPSES;

EID: 84930182225     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.3361     Document Type: Article

References (35)

1. Denk, W., Briggman, K. L. & Helmstaedter, M. Structural neurobiology: missing link to a mechanistic understanding of neural computation. Nat. Rev. Neurosci. 13, 351-358 (2012).
2. Lichtman, J. W. & Denk, W. The big and the small: challenges of imaging the brain's circuits. Science 334, 618-623 (2011).
3. Jarrell, T. A. et al. The connectome of a decision-making neural network. Science 337, 437-444 (2012).
4. Briggman, K. L., Helmstaedter, M. & Denk, W. Wiring specificity in the direction-selectivity circuit of the retina. Nature 471, 183-188 (2011).
5. Bock, D. D. et al. Network anatomy and in vivo physiology of visual cortical neurons. Nature 471, 177-182 (2011).
6. Knott, G., Marchman, H., Wall, D. & Lich, B. Serial section scanning electron microscopy of adult brain tissue using focused ion beam milling. J. Neurosci. 28, 2959-2964 (2008).
7. Mikula, S., Binding, J. & Denk, W. Staining and embedding the whole mouse brain for electron microscopy. Nat. Methods 9, 1198-1201 (2012).
8. Li, A. et al. Micro-optical sectioning tomography to obtain a high-resolution atlas of the mouse brain. Science 330, 1404-1408 (2010).
9. Willingham, M. C. & Rutherford, A. V. The use of osmium-thiocarbohydrazide-osmium (OTO) and ferrocyanide-reduced osmium methods to enhance membrane contrast and preservation in cultured cells. J. Histochem. Cytochem. 32, 455-460 (1984).
10. Deerinck, T. J. et al. Enhancing serial block-face scanning electron microscopy to enable high resolution 3-D nanohistology of cells and tissues. Microsc. Microanal. 16, 1138-1139 (2010).
11. Zweens, J., Frankena, H., Rispens, P. & Zijlstra, W. G. Determination of extracellular fluid volume in the dog with ferrocyanide. Pflugers Arch. 357, 275-290 (1975).
12. Van Harreveld, A., Crowell, J. & Malhotra, S. K. A study of extracellular space in central nervous tissue by freeze-substitution. J. Cell Biol. 25, 117-137 (1965).
13. Thorne, R. G. & Nicholson, C. In vivo diffusion analysis with quantum dots and dextrans predicts the width of brain extracellular space. Proc. Natl. Acad. Sci. USA 103, 5567-5572 (2006).
14. Van Harreveld, A. in The Structure and Function of Nervous Tissue (ed. Bourne, G. H. ) Ch. 10, 447-511 (Academic Press, 1972).
15. Cragg, B. Preservation of extracellular space during fixation of the brain for electron microscopy. Tissue Cell 12, 63-72 (1980).
16. Rivlin, P. K. & Raymond, P. A. Use of osmium tetroxide-potassium ferricyanide in reconstructing cells from serial ultrathin sections. J. Neurosci. Methods 20, 23-33 (1987).
17. Karnovsky, M. J. Use of ferrocyanide-reduced osmium tetroxide in electron microscopy. Proc. Am. Soc. J. Cell Biol. 51, 146a (1971).
18. Alexander, R., Ko, E. C. F., Mac, Y. C. & Parker, A. J. Solvation of ions. XI. Solubility products and instability constants in water methanol, formamide, dimethylformamide, dimethylacetamide, dimethyl sulfoxide, acetonitrile, and hexamethylphosphorotriamide. J. Am. Chem. Soc. 89, 3703-3712 (1967).
19. Denk, W. & Horstmann, H. Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure. PLoS Biol. 2, e329 (2004).
20. Bahr, G. F. Osmium tetroxide and ruthenium tetroxide and their reactions with biologically important substances: electron stains III. Exp. Cell Res. 7, 457-479 (1954).
21. Hayat, M. A. Principles and Techniques of Electron Microscopy: Biological Applications 4th edn. (Cambridge Univ. Press, 2000).
22. Greedan, J. E., Willson, D. B. & Haas, T. E. Metallic nature of osmium dioxide. Inorg. Chem. 7, 2461-2463 (1968).
23. Eberle, A. L. et al. High-resolution, high-throughput imaging with a multibeam scanning electron microscope. J. Microsc. doi:10. 1111/jmi. 12224 (27 January 2015).
24. Titze, B. & Denk, W. Automated in-chamber specimen coating for serial block-face electron microscopy. J. Microsc. 250, 101-110 (2013).
25. Pfenninger, K. H. The cytochemistry of synaptic densities. I. An analysis of the bismuth iodide impregnation method. J. Ultrastruct. Res. 34, 103-122 (1971).
26. Tapia, J. C. et al. High-contrast en bloc staining of neuronal tissue for field emission scanning electron microscopy. Nat. Protoc. 7, 193-206 (2012).
27. Helmstaedter, M., Briggman, K. L. & Denk, W. High-accuracy neurite reconstruction for high-throughput neuroanatomy. Nat. Neurosci. 14, 1081-1088 (2011).
28. Tomassy, G. S. et al. Distinct profiles of myelin distribution along single axons of pyramidal neurons in the neocortex. Science 344, 319-324 (2014).
29. Hayworth, K. J. et al. Ultrastructurally smooth thick partitioning and volume stitching for large-scale connectomics. Nat. Methodsdoi:10. 1038/nmeth. 3292 (16 February 2015).
30. Hayworth, K. J., Kasthuri, N., Schalek, R. & Lichtman, J. W. Automating the collection of ultrathin serial sections for large volume TEM reconstructions. Microsc. Microanal. 12, 86-87 (2006).
31. Siegel, S. M. & Siegel, B. Z. Autoxidation of pyrogallol: general characteristics and inhibition by catalase. Nature 181, 1153-1154 (1958).
32. Spurr, A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31-43 (1969).
33. Binding, J., Mikula, S. & Denk, W. Low-dosage maximum-a-posteriori focusing and stigmation (MAPFoSt). Microsc. Microanal. 19, 38-55 (2013).
34. Gundersen, H. J. G. et al. The new stereological tools: disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis. APMIS 96, 857-881 (1988).
35. Fiala, J. C. & Harris, K. M. Extending unbiased stereology of brain ultrastructure to three-dimensional volumes. J. Am. Med. Inform. Assoc. 8, 1-16 (2001).