Staining and embedding the whole mouse brain for electron microscopy

Nature Methods

Volumn 9, Issue 12, 2012, Pages 1198-1201

  Mikula, Shawn ^a   Binding, Jonas ^a   Denk, Winfried ^a  

^a MAX PLANCK INSTITUTE FOR MEDICAL RESEARCH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

MYELIN;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN; BRAIN MAPPING; CONTROLLED STUDY; ELECTRON MICROSCOPY; EMBEDDING; MOUSE; NERVE FIBER; NONHUMAN; PRIORITY JOURNAL; STAINING; TISSUE PRESERVATION;

ANIMALS; AXONS; BRAIN; MALE; MICE; MICROSCOPY, ELECTRON; NERVE FIBERS, MYELINATED; OSMIUM TETROXIDE; STAINING AND LABELING; TISSUE EMBEDDING;

EID: 84870784136     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.2213     Document Type: Article

References (36)

1. Kasthuri, N. & Lichtman, J.W. Nat. Methods 4, 307-308 (2007).
2. Bohland, J.W. et al. PLoS Comput. Biol. 5, e1000334 (2009).
3. Tsai, P.S. et al. J. Neurosci. 29, 14553-14570 (2009).
4. Sabatini, D.D., Bensch, K. & Barrnett, R.J. J. Cell Biol. 17, 19-58 (1963).
5. Dalton, A.J., Kahler, H., Striebich, M.J. & Lloyd, B. J. Natl. Cancer Inst. 11, 439-461 (1950).
6. Palay, S.L., McGee-Russell, S.M., Gordon, S. Jr. & Grillo, M.A. J. Cell Biol. 12, 385-410 (1962).
7. Seligman, A.M., Wasserkrug, H.L. & Hanker, J.S. J. Cell Biol. 30, 424-432 (1966).
8. Li, A. et al. Science 330, 1404-1408 (2010).
9. Chung, J.R. et al. Front. Neuroinform. 5, 29 (2011).
10. Dempster, W.T. Am. J. Anat. 107, 59-72 (1960).
11. Hagström, L. & Bahr, G.F. Histochem. Cell Biol. 2, 1-4 (1960).
12. Bozzola, J.J. & Russell, L.D. Electron Microscopy: Principles and Techniques for Biologists. (Jones & Bartlett Learning, 1999).
13. Hayat, M.A. Principles and Techniques of Electron Microscopy: Biological Applications, 4th edn. (Cambridge University Press, 2000).
14. Van Holde, K.E. J. Phys. Chem. 63, 1574-1577 (1959).
15. Bahr, G.F. Exp. Cell Res. 7, 457-479 (1954).
16. Guha, P.C. & De, S.C. J. Chem. Soc. Trans. 125, 1215-1218 (1924).
17. De Bruijn, W.C. in Proc. 4th Eur. Reg. Conf. Electron Microsc. (ed. Bocciarelli, D.S.) 11-65 (Rome: Tipografa Polyglotta Vaticana, 1968).
18. Karnovsky, M.J. in Proc. 11th Meet. Am. Soc. Cell Biol. 146 (1971).
19. Seligman, A.M., Hanker, J.S., Wasserkrug, H., Dmochowski, H. & Katzoff, L. J. Histochem. Cytochem. 13, 629-639 (1965).
20. Quarles, R.H., Macklin, W.B. & Morell, P. in Basic Neurochemistry: Molecular, Cellular, and Medical Aspects, 7th edn. (eds. Siegel, G., Albers, R.W., Brady, S. & Price, D.) Ch. 4, 51-72 (Academic, 2006).
21. Spurr, A.R. J. Ultrastruct. Res. 26, 31-43 (1969).
22. Kushida, H. J. Electron Microsc. (Tokyo) 23, 197 (1974).
23. Partadiredja, G., Miller, R. & Oorschot, D.E. J. Neurocytol. 32, 1165-1179 (2003).
24. Sturrock, R.R. Neuropathol. Appl. Neurobiol. 6, 415-420 (1980).
25. Denk, W. & Horstmann, H. PLoS Biol. 2, e329 (2004).
26. Terzakis, J.A. J. Ultrastruct. Res. 22, 168-184 (1968).
27. Walton, J. J. Histochem. Cytochem. 27, 1337-1342 (1979).
28. Willingham, M.C. & Rutherford, A.V. J. Histochem. Cytochem. 32, 455-460 (1984).
29. Deerinck, T.J. et al. Microsc. Microanal. 16 (suppl. 2), 1138-1139 (2010).
30. Luft, J.H. Anat. Rec. 133, 305 (1959).
31. Binding, J., Mikula, S. & Denk, W. Microsc. Microanal. (in the press).
32. Holland, P.W. & Welsch, R.E. Commun. Stat. Theory Methods 6, 813-827 (1977).
33. Baumann, M. et al. in Proc. 6th PIMS Ind. Problem Solving Workshop (ed. Macki, J.) Ch. 1, 1-25 (PIMS, 2002).
34. Mikula, S., Trotts, I., Stone, J.M. & Jones, E.G. Neuroimage 35, 9-15 (2007).
35. Reynolds, E.S. J. Cell Biol. 17, 208-212 (1963).
36. Franklin, K.B.J. & Paxinos, G. The Mouse Brain in Stereotaxic Coordinates 3rd edn. (Academic, 2008).