Image-based cell-resolved screening assays in flow

Cytometry Part A

Volumn 87, Issue 6, 2015, Pages 541-548

  Cheung, Man Ching ^a   McKenna, Brian ^a   Wang, Steve S ^a   Wolf, Dane ^a   Ehrlich, Daniel J ^a  

^a Boston University   (United States)

Author keywords

HCA; High content; High throughput; Imaging; Live cell assay; Microfluidic; Receptor capping; Screening

Indexed keywords

GREEN FLUORESCENT PROTEIN; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN;

ALGORITHM; ANIMAL; BIOLOGY; CARCINOMA, HEPATOCELLULAR; CHO CELL LINE; CRICETULUS; DEVICES; DRUG DEVELOPMENT; FLOW CYTOMETRY; HIGH THROUGHPUT SCREENING; HUMAN; IMAGE PROCESSING; JURKAT CELL LINE; LIVER NEOPLASMS; MICROFLUIDIC ANALYSIS; PHYSIOLOGY; PROCEDURES; SYSTEMS BIOLOGY; T LYMPHOCYTE; TUMOR CELL LINE;

ALGORITHMS; ANIMALS; CARCINOMA, HEPATOCELLULAR; CELL LINE, TUMOR; CHO CELLS; COMPUTATIONAL BIOLOGY; CRICETULUS; DRUG DISCOVERY; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; JURKAT CELLS; LIVER NEOPLASMS; MICROFLUIDIC ANALYTICAL TECHNIQUES; NF-KAPPA B; SYSTEMS BIOLOGY; T-LYMPHOCYTES;

EID: 84929961185     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22609     Document Type: Article

References (20)

1. Mckenna BK, Evans JG, Cheung MC, Ehrlich DJ. A parallel microfluidic flow cytometer for high-content screening. Nat Methods 2011;8:401-403.
2. Taylor DL, Haskins JR, Giuliano KA. High Content Screening. Totowa, NJ: Humana Press; 2007.
3. Carpenter AE. Image-based chemical screening. Nat Chem Biol 2007;3:461-465.
4. Loo L, Wu L, Altschuler S. Image-based multivariate profiling of drug responses from single cells. Nat Methods 2007;4:445-453.
5. Zanella F, Lorens JB, Link W. High content screening: Seeing is believing. Trends Biotechnol 2010;28:237-245.
6. Young DW, Bender A, Hoyt J, McWhinnie E, Chirn GW, Tao C, Tallarico JA, Labow M, Jenkins JL, Mitchinson TJ, Feng Y. Integrating high-content screening and ligand-target prediction to identify mechanism of action. Nat Chem Biol 2008;4:59-68.
7. Zuba-Surma EK, Kucia M, Abdel-Latif A, Lillard JW, Ratajczak MZ. The ImageStream System: A key step to a new era in imaging. Folia Histochem Cytobiol 2007;45:279-290.
8. Bray M-A, Fraser AN, Hasaka TP, Carpenter AE. Workflow and metrics for image quality control in large-scale high-content screens. J Biomol Screen 2012;17:266-274.
9. Bray M-A, Carpenter AE. Advanced Assay Development Guidelines for Image-Based High Content Screening and Analysis. Assay Guidance Manual. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004.
10. Gorenstein J, Zack B, Marszalek J, Bagchi A, Subramaniam S, Carroll P, Elbi C. Reducing the multidimensionality of high-content screening into versatile powerful descriptors. Biotechniques 2010;49:663-665.
11. Zhang J-H, Chung TDY, Oldenburg KR. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 1999;4:67-73.
12. Mckenna BK, Selim AA, Richard Bringhurst F, Ehrlich DJ. 384-channel parallel microfluidic cytometer for rare-cell screening. Lab Chip 2009;9:305-310.
13. Shariff A, Kangas J, Coelho LP, Quinn S, Murphy RF. Automated image analysis for high-content screening and analysis. J Biomol Screen 2010;15:726-734.
14. Ng AYJ, Rajapakse JC, Welsch RE, Matsudaira PT, Horodincu V, Evans JG. A cell profiling framework for modeling drug responses from HCS imaging. J Biomol Screen 2010;15:858-868.
15. Perlman ZE, Slack MD, Feng Y, Mitchison TJ, Wu LF, Altschuler SJ. Multidimensional drug profiling by automated microscopy. Science 2004;306:1194-1198.
16. Kümmel A, Selzer P, Beibel M, Gubler H, Parker CN, Gabriel D. Comparison of multivariate data analysis strategies for high-content screening. J Biomol Screen 2011;16:338-347.
17. Bertelsen M, Sanfridson A. Inflammatory pathway analysis using a high content screening platform. Assay Drug Dev Technol 2005;3:261-271.
18. Borchert KM, Galvin RJS, Frolik CA, Hale LV, Halladay DL, Gonyier RJ, Trask OJ, Nickischer DR, Houck KA. High-content screening assay for activators of the Wnt/Fzd pathway in primary human cells. Assay Drug Dev Technol 2005;3:133-141.
19. Freeley M, Bakos G, Davies A, Kelleher D, Long A, Dunican DJ. A high-content analysis toolbox permits dissection of diverse signaling pathways for T lymphocyte polarization. J Biomol Screen 2010;15:541-555.
20. Kammer GM, Smith JA, Mitchell R. Capping of human T cell specific determinants: Kinetics of capping and receptor re-expression and regulation by the cytoskeleton. J Immunol 1983;130:38-44.