Avoiding false positive antigen detection by flow cytometry on blood cell derived microparticles: The importance of an appropriate negative control

PLoS ONE

Volumn 10, Issue 5, 2015, Pages

  Crompot, Emerence ^a   Van Damme, Michael ^a   Duvillier, Hugues ^a   Pieters, Karlien ^a   Vermeesch, Marjorie ^b   Perez Morga, David ^b   Meuleman, Nathalie ^a   Mineur, Philippe ^c   Bron, Dominique ^a   Lagneaux, Laurence ^a   Stamatopoulos, Basile ^a  

^a JULES BORDET INSTITUTE   (Belgium)

^b UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

^c Service des urgences Grand Hôpital de Charleroi   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

BETA3 INTEGRIN; FIBRINOGEN RECEPTOR; LIPOCORTIN 5; LEUKOCYTE ANTIGEN; MICROSPHERE; MONOCLONAL ANTIBODY;

ANTIGEN DETECTION; ANTIGEN EXPRESSION; ARTICLE; B LYMPHOCYTE; BLOOD CELL; CELL LABELING; CONTROLLED STUDY; CYTOMETRY; FALSE POSITIVE RESULT; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LATEX BEAD CYTOMETRY; LEUKEMIA CELL; MEMBRANE MICROPARTICLE; MONOCYTE; NATURAL KILLER CELL; T LYMPHOCYTE; THROMBOCYTE; VALIDATION PROCESS; CHRONIC LYMPHATIC LEUKEMIA; IMMUNOLOGICAL PROCEDURES; IMMUNOLOGY; LYMPHOCYTE; PROCEDURES;

ANNEXIN A5; ANTIBODIES, MONOCLONAL; ANTIGENS, CD; BLOOD PLATELETS; CELL-DERIVED MICROPARTICLES; FALSE POSITIVE REACTIONS; FLOW CYTOMETRY; HUMANS; IMMUNOLOGIC TESTS; LEUKEMIA, LYMPHOCYTIC, CHRONIC, B-CELL; LYMPHOCYTES; MICROSPHERES; MONOCYTES;

EID: 84929377691     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0127209     Document Type: Article

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