Flow cytometric analysis of microparticle phenotype and their role in thrombin generation

Cytometry Part B - Clinical Cytometry

Volumn 80 B, Issue 1, 2011, Pages 57-63

  MacEy, M G ^a   Enniks, N ^a   Bevan, S ^a  

^a ROYAL LONDON HOSPITAL   (United Kingdom)

Author keywords

Calibrated automated thrombogram VR (CAT) thrombin generation assay; CD15; CD45; plasma filtration; Terms

Indexed keywords

CD142 ANTIGEN; CD15 ANTIGEN; CD45 ANTIGEN; GLYCOPHORIN A; GLYCOPROTEIN IB ALPHA; LIPOCORTIN 5; LYMPHOCYTE ANTIGEN; PHOSPHATIDYLSERINE; THROMBIN; THROMBOPLASTIN; UNCLASSIFIED DRUG;

ADULT; ANTIGEN BINDING; ANTIGEN EXPRESSION; ARTICLE; BLOOD FILTRATION; BLOOD SAMPLING; CONTROLLED STUDY; ERYTHROCYTE FUNCTION AND CHARACTERISTICS; FEMALE; FLOW CYTOMETRY; HUMAN; LEUKOCYTE FUNCTION; LEUKOCYTE MICROPARTICLE; MALE; MEMBRANE MICROPARTICLE; NORMAL HUMAN; PHENOTYPE; PLATELET MICROPARTICLE; PRIORITY JOURNAL; PROTEIN EXPRESSION; THROMBIN TIME; THROMBOCYTE FUNCTION;

ADULT; ANTIGENS, CD15; ANTIGENS, CD45; CELL-DERIVED MICROPARTICLES; FEMALE; FLOW CYTOMETRY; GLYCOPHORIN; HUMANS; IMMUNOPHENOTYPING; MALE; MIDDLE AGED; PHENOTYPE; PLATELET GLYCOPROTEIN GPIB-IX COMPLEX; THROMBIN; YOUNG ADULT;

EID: 78650819812     PISSN: 15524949     EISSN: 15524957     Source Type: Journal    
DOI: 10.1002/cyto.b.20551     Document Type: Article

References (17)

1. Flaumenhaft R, Dilks JR, Richardson J, Alden E, Patel Hett SR, Battinelli E, Klement GL, Sola Visner M, Italiano JE Jr,. Megakaryocyte derived microparticles: Direct visualization and distinction from platelet derived microparticles. Blood 2009; 113: 1112 - 1121.
2. Flaumenhaft R,. Formation and fate of platelet microparticles. Blood Cells Mol Dis 2006; 36: 182 - 187.
3. Berckmans RJ, Nieuwland R, Böing AN, Romijn F.P.H.T.M, Hack CE, Sturk A,. Cell derived microparticles circulate in healthy humans and support low grade thrombin generation. Thrombosis Haemostasis 2001; 85: 639 - 646.
4. Sims PJ, Wiedmer T, Esmon CT, Weissll HJ, Shattil SJ,. Assembly of the platelet prothrombinase complex is linked to vesiculation of the platelet plasma membrane. Studies in Scott syndrome: An isolated defect in platelet procoagulant activity. J Biol Chem 1989; 264: 17049 - 17057.
5. Morel O, Toti F, Hugel B, Bakouboula B, Camoin Jau L, Dignat George F, Freyssinet JM,. Procoagulant microparticles disrupting the vascular homeostasis equation? Arteriosclerosis Thrombosis Vascular Biol 2006; 26: 2594 - 2604.
6. del Conde I, Shrimpton C.N, Thiagarajan P, López JA,. Tissue factor bearing microvesicles arise from lipid rafts and fuse with activated platelets to initiate coagulation. Blood 2005b; 106: 1604 - 1611.
7. Steppich B, Mattisek C, Sobczyk D, Kastrati A, Schomig A, Ott I,. Tissue factor pathway inhibitor on circulating microparticles in acute myocardial infarction. Thromb Haemost 2005; 93: 35 - 39.
8. Pérez Casal M, Downey C, Fukudome K, Marx G, Toh CH,. Activated protein C induces the release of microparticle associated endothelial protein C receptor. Blood 2005; 105: 1515 - 1522.
9. Baglin T,. The measurement and application of thrombin generation. Br J Haematol 2005; 130: 653 - 661.
10. Eichinger S, Hron G, Kollars M, Kyrle PA,. Prediction of recurrent venous thromboembolism by endogenous thrombin potential and D Dimer. Clin Chem 2008; 54: 2042 - 2048.
11. Macey MG, Bevan S, Alam S, Verghese L, Agrawal S, Beski S, Thuraisingham R, Maccallum PK,. Platelet activation and endogenous thrombin potential in pre eclampsia. Thromb Res 2010; 125: e76 - e81.
12. Macey M, McCarthy D, Azam U, Milne T, Golledge P, Newland AC, Ethylenediaminetetraacetic acid plus citrate theophylline adenosine dipyridamole (EDTA CTAD): A novel anticoagulant for the flow cytometric assessment of platelet and neutrophil activation ex vivo in whole blood. Cytometry B Clin Cytom B 2003; 51: 30 - 40.
13. McCarthy DA, Macey MG, Allen PD,. A simple, novel, procedure for monitoring membrane scrambling and permeability in microparticles, platelets, and leukocytes in whole blood samples. Exp Hematol 2008; 36: 909 - 921.
14. Lawrie AS, Harrison P, Cardigan RA, Mackie IJ,. The characteriszation and impact of microparticles on haemostasis within fresh frozen plasma. Vox Sang 2008; 95: 197 - 204.
15. Sinauridze EI, Kireev DA, Popenko NY, Pichugin AV, Panteleev MA, Krymskaya OV, Ataullakhanov FL,. Platelet microparticle membranes have 50 to 100 fold higher specific procoagumant activity than activated platelets. Thromb Haemost 2007; 97: 425 - 434.
16. Stampfuss JJ, Censarek P, Bein D, Schror K, Grandoch M, Naber C, Weber AA,. Membrane environment rather than tissue factor expression determines thrombin formation triggered by monocytic cells undergoing apoptosis. J Leukoc Biol 2008; 83: 1379 - 1381.
17. Butenas S, Bouchard BA, Brummeo Ziedens KE, Parhami Seren B, Mann KG,. Tissue factor activity in whole blood. Blood 2005; 105: 2764 - 2770.