Isotype controls in phenotyping and quantification of microparticles: A major source of error and how to evade it

Thrombosis Research

Volumn 122, Issue 5, 2008, Pages 691-700

  Trummer, Arne ^a   De Rop, Christiane ^a   Tiede, Andreas ^a   Ganser, Arnold ^a   Eisert, Roswith ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Flow cytometry; Isotype controls; Microparticles; P Selectin

Indexed keywords

ANTIBODY; FLUORESCEIN ISOTHIOCYANATE; MEMBRANE ANTIGEN; PADGEM PROTEIN; PHYCOERYTHRIN;

ANALYTICAL ERROR; ANTIBODY LABELING; ARTICLE; CALIBRATION; CELL CULTURE; CELL STRAIN HL 60; CONTROL STRATEGY; CONTROLLED STUDY; CULTURE MEDIUM; ENDOTHELIAL MICROPARTICLE; ENDOTHELIUM CELL; FALSE POSITIVE RESULT; FLOW CYTOMETRY; FLUORESCENCE; HUMAN; HUMAN CELL; INSTRUMENTATION; ISOTYPE CONTROL; LEUKEMIA CELL LINE; MEMBRANE MICROPARTICLE; NORMAL HUMAN; PHENOTYPE; PLATELET MICROPARTICLE; PRIORITY JOURNAL; QUANTITATIVE ASSAY; RELIABILITY; REPRODUCIBILITY; SIGNAL DETECTION; STAINING; UMBILICAL VEIN;

ANTIBODIES, MONOCLONAL; ANTIGENS, SURFACE; BLOOD PLATELETS; CELL LINE; CELL-DERIVED MICROPARTICLES; ENDOTHELIAL CELLS; FALSE POSITIVE REACTIONS; FLOW CYTOMETRY; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; HUMANS; LEUKEMIA; P-SELECTIN; PHENOTYPE; PHYCOERYTHRIN; STAINING AND LABELING;

EID: 49549085696     PISSN: 00493848     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.thromres.2008.01.005     Document Type: Article

References (21)

1. Simak J., and Gelderman M.P. Cell membrane microparticles in blood and blood products: potentially pathogenic agents and diagnostic markers. Transfus Med Rev 20 (2006) 1-26
2. Jy W., Horstman L.L., Jimenez J.J., Ahn Y.S., Biro E., Nieuwland R., et al. Measuring circulating cell-derived microparticles. J Thromb Haemost 2 (2004) 1842-1843
3. Ahn Y.S., Jy W., Jimenez J.J., and Horstman L.L. More on: cellular microparticles: what are they bad or good for?. J Thromb Haemost 2 (2004) 1215-1216
4. Keeney M., Gratama J.W., Chin-Yee I.H., and Sutherland D.R. Isotype controls in the analysis of lymphocytes and CD34+ stem and progenitor cells by flow cytometry - time to let go!. Cytometry 34 (1998) 280-283
5. Scheinichen D., Kleine H.D., Koksch M., Brandl M., Heine J., Elsnar H.A., et al. Comparison of four blood sampling sites for flow-cytometric analysis of platelet function. Transfus Med Hemother 30 (2003) 109-114
6. Kim H.K., Song K.S., Lee E.S., Lee Y.J., Park Y.S., Lee K.R., et al. Optimized flow cytometric assay for the measurement of platelet microparticles in plasma: pre-analytic and analytic considerations. Blood Coagul Fibrinolysis 13 (2002) 393-397
7. Bode A.P., and Hickerson D.H. Characterization and quantitation by flow cytometry of membranous microparticles formed during activation of platelet suspensions with ionophore or thrombin. Platelets 11 (2000) 259-271
8. Baranska P., Jerczynska H., Pawlowska Z., Koziolkiewicz W., and Cierniewski C.S. Expression of integrins and adhesive properties of human endothelial cell line EA.hy 926. Cancer Genomics Proteomics 2 (2005) 265-270
9. Abid Hussein M.N., Meesters E.W., Osmanovic N., Romijn F.P., Nieuwland R., and Sturk A. Antigenic characterization of endothelial cell-derived microparticles and their detection ex vivo. J Thromb Haemost 1 (2003) 2434-2443
10. Garcia B.A., Smalley D.M., Cho H., Shabanowitz J., Ley K., and Hunt D.F. The platelet microparticle proteome. J Proteome Res 4 (2005) 1516-1521
11. Cambien B., and Wagner D.D. A new role in hemostasis for the adhesion receptor P-selectin. Trends Mol Med 10 (2004) 179-186
12. Furie B., and Furie B.C. Role of platelet P-selectin and microparticle PSGL-1 in thrombus formation. Trends Mol Med 10 (2004) 171-178
13. Polgar J., Matuskova J., and Wagner D.D. The P-selectin, tissue factor, coagulation triad. J Thromb Haemost 3 (2005) 1590-1596
14. Blann A.D., Nadar S.K., and Lip G.Y. The adhesion molecule P-selectin and cardiovascular disease. Eur Heart J 24 (2003) 2166-2179
15. Banfi C., Brioschi M., Wait R., Begum S., Gianazza E., Pirillo A., et al. Proteome of endothelial cell-derived procoagulant microparticles. Proteomics 5 (2005) 4443-4455
16. Bernal-Mizrachi L., Jy W., Jimenez J.J., Pastor J., Mauro L.M., Horstman L.L., et al. High levels of circulating endothelial microparticles in patients with acute coronary syndromes. Am Heart J 145 (2003) 962-970
17. Morel O., Toti F., Hugel B., Bakouboula B., Camoin-Jau L., Dignat-George F., et al. Procoagulant microparticles: disrupting the vascular homeostasis equation?. Arterioscler Thromb Vasc Biol 26 (2006) 2594-2604
18. Osterud B., and Bjorklid E. Sources of tissue factor. Semin Thromb Hemost 32 (2006) 11-23
19. Filshie R.J., Zannettino A.C., Makrynikola V., Gronthos S., Henniker A.J., Bendall L.J., et al. MUC18, a member of the immunoglobulin superfamily, is expressed on bone marrow fibroblasts and a subset of hematological malignancies. Leukemia 12 (1998) 414-421
20. Lynch S.F., and Ludlam C.A. Plasma microparticles and vascular disorders. Br J Haematol 137 (2007) 36-48
21. Reininger A.J., Heijnen H.F., Schumann H., Specht H.M., Schramm W., and Ruggeri Z.M. Mechanism of platelet adhesion to von Willebrand factor and microparticle formation under high shear stress. Blood 107 (2006) 3537-3545