Imaging windows for long-term intravital imaging: General overview and technical insights

IntraVital

Volumn 3, Issue 2, 2014, Pages

  Alieva, Maria ^a   Ritsma, Laila ^b,c   Giedt, Randy J ^d   Weissleder, Ralph ^b,d   Van Rheenen, Jacco ^a  

^a UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

^b HARVARD MEDICAL SCHOOL   (United States)

^c MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^d MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

Abdominal imaging window; Cranial imaging window; Dorsal skinfold chamber; intravital microscopy; Mammary imaging window; Surgery

Indexed keywords

BUPRENORPHINE; HYPNORM; ISOFLURANE; KETAMINE; MIDAZOLAM; XYLAZINE;

ARTICLE; ATTITUDE; BEHAVIOR; BODY CONSTITUTION; BODY POSTURE; CANCER RESEARCH; HUMAN; IMAGING; IMPLANTATION; INTRAVITAL IMAGING; LOCOMOTION; NONHUMAN; PERIOPERATIVE PERIOD; POSTOPERATIVE CARE; PREOPERATIVE CARE; VITAL SIGN;

ANIMALIA;

EID: 84929373209     PISSN: 21659079     EISSN: 21659087     Source Type: Journal    
DOI: 10.4161/intv.29917     Document Type: Article

References (104)

1. Condeelis J, Weissleder R. In vivo imaging in cancer. Cold Spring Harb Perspect Biol 2010; 2:a003848; PMID:20861158; http://dx.doi.org/10.1101/cshperspect.a003848
2. Ritsma L, Ponsioen B, Van Rheenen J. Intravital imaging of cell signaling in mice. IntraVital 2012; 1:2-10; http://dx.doi.org/10.4161/intv.20802
3. Pittet MJ, Weissleder R. Intravital imaging. Cell 2011; 147:983-91; PMID:22118457; http://dx.doi.org/10.1016/j.cell.2011.11.004
4. Ellenbroek SI, van Rheenen J. Imaging hallmarks of cancer in living mice. Nat Rev Cancer 2014; 14:406-18; PMID:24854083; http://dx.doi.org/10.1038/nrc3742
5. Fein MR, Egeblad M. Caught in the act: revealing the metastatic process by live imaging. Dis Model Mech 2013; 6:580-93; PMID:23616077; http://dx.doi.org/10.1242/dmm.009282
6. Ewald AJ, Werb Z, Egeblad M. Preparation of mice for long-term intravital imaging of the mammary gland. Cold Spring Harb Protoc 2011; 2011:t5562; PMID:21285262; http://dx.doi.org/10.1101/pdb.prot5562
7. Garnett MJ, Edelman EJ, Heidorn SJ, Greenman CD, Dastur A, Lau KW, Greninger P, Thompson IR, Luo X, Soares J, et al. Systematic identification of genomic markers of drug sensitivity in cancer cells. Nature 2012; 483:570-5; PMID:22460902; http://dx.doi.org/10.1038/nature11005
8. Wyckoff J, Gligorijevic B, Entenberg D, Segall J, Condeelis J. High-resolution multiphoton imaging of tumors in vivo. Cold Spring Harb Protoc 2011; 2011:1167-84; PMID:21969629; http://dx.doi.org/10.1101/pdb.top065904
9. Zomer A, Beerling E, Vlug EJ, van Rheenen J. Realtime intravital imaging of cancer models. Clin Transl Oncol 2011; 13:848-54; PMID:22126727; http://dx.doi.org/10.1007/s12094-011-0745-3
10. Hefendehl JK, Wegenast-Braun BM, Liebig C, Eicke D, Milford D, Calhoun ME, Kohsaka S, Eichner M, Jucker M. Long-term in vivo imaging of ß-amyloid plaque appearance and growth in a mouse model of cerebral ß-amyloidosis. J Neurosci 2011; 31:624-9; PMID:21228171; http://dx.doi.org/10.1523/JNEUROSCI.5147-10.2011
11. Zomer A, Ellenbroek SI, Ritsma L, Beerling E, Vrisekoop N, Van Rheenen J. Intravital imaging of cancer stem cell plasticity in mammary tumors. Stem Cells 2013; 31:602-6; PMID:23225641; http://dx.doi.org/10.1002/stem.1296
12. Ritsma L, Ellenbroek SI, Zomer A, Snippert HJ, de Sauvage FJ, Simons BD, Clevers H, van Rheenen J. Intestinal crypt homeostasis revealed at singlestem-cell level by in vivo live imaging. Nature 2014; 507:362-5; PMID:24531760; http://dx.doi.org/10.1038/nature12972
13. Ravina F. De motu cerebri. Memoires de l'Academie des Sciences de Turin 1811; 20:61-93
14. Donders FC. De bewegingen der Hersenen en de veranderingen der vaatvulling van der "Pia Mater", ook bijgesloten onuitzetberen schedel regstreeks onderzocht. Nederlandsche Lancet 1850; 5:521-53
15. Forbes HS. The cerebral circulation: I. observation and measurement of pial vessels. Arch Neurol Psychiatry 1928; 19:751-61; http://dx.doi.org/10.1001/archneurpsyc.1928.02210110003001
16. Holtmaat A, Bonhoeffer T, Chow DK, Chuckowree J, De Paola V, Hofer SB, Hübener M, Keck T, Knott G, Lee WC, et al. Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window. Nat Protoc 2009; 4:1128-44; PMID:19617885; http://dx.doi.org/10.1038/nprot.2009.89
17. Grutzendler J, Kasthuri N, Gan WB. Long-term dendritic spine stability in the adult cortex. Nature 2002; 420:812-6; PMID:12490949; http://dx.doi.org/10.1038/nature01276
18. Mostany R, Portera-Cailliau C. A method for 2-photon imaging of blood flow in the neocortex through a cranial window. J Vis Exp 2008; 678; PMID:19066563; http://dx.doi.org/10.3791/678
19. Dreier JP, Victorov IV, Petzold GC, Major S, Windmüller O, Fernández-Klett F, Kandasamy M, Dirnagl U, Priller J. Electrochemical failure of the brain cortex is more deleterious when it is accompanied by low perfusion. Stroke 2013; 44:490-6; PMID:23287786; http://dx.doi.org/10.1161/STROKEAHA.112.660589
20. Miyauchi T, Wei EP, Povlishock JT. Therapeutic targeting of the axonal and microvascular change associated with repetitive mild traumatic brain injury. J Neurotrauma 2013; 30:1664-71; PMID:23796228; http://dx.doi.org/10.1089/neu.2013.2995
21. Murmu RP, Li W, Holtmaat A, Li JY. Dendritic spine instability leads to progressive neocortical spine loss in a mouse model of Huntington's disease. J Neurosci 2013; 33:12997-3009; PMID:23926255; http://dx.doi.org/10.1523/JNEUROSCI.5284-12.2013
22. Kienast Y, von Baumgarten L, Fuhrmann M, Klinkert WE, Goldbrunner R, Herms J, Winkler F. Real-time imaging reveals the single steps of brain metastasis formation. Nat Med 2010; 16:116-22; PMID:20023634; http://dx.doi.org/10.1038/nm.2072
23. Algire GH. An Adaptation of the Transparent-Chamber Technique to the Mouse. J Natl Cancer Inst 1943; 4:1-11
24. Sandison J. a new method for the microscopic study of living growing tissues by the introduction of a transparent chamber in the rabbit's ear. Anat Rec 1924; 28:281-7; http://dx.doi.org/10.1002/ar.1090280403
25. Lehr HA, Leunig M, Menger MD, Nolte D, Messmer K. Dorsal skinfold chamber technique for intravital microscopy in nude mice. Am J Pathol 1993; 143:1055-62; PMID:7692730
26. Alexander S, Koehl GE, Hirschberg M, Geissler EK, Friedl P. Dynamic imaging of cancer growth and invasion: a modified skin-fold chamber model. Histochem Cell Biol 2008; 130:1147-54; PMID:18987875; http://dx.doi.org/10.1007/s00418-008-0529-1
27. Sckell A, Leunig M. The Dorsal Skinfold Chamber: Studying Angiogenesis by Intravital Microscopy. Methods in Molecular Biology, Angiogenesis Protocols, Second Edition, Vol 467 Humana Press 2009
28. Menger MD, Vollmar B. In vivo analysis of microvascular reperfusion injury in striated muscle and skin. Microsurgery 1994; 15:383-9; PMID:7968463; http://dx.doi.org/10.1002/micr.1920150605
29. Kedrin D, Gligorijevic B, Wyckoff J, Verkhusha VV, Condeelis J, Segall JE, van Rheenen J. Intravital imaging of metastatic behavior through a mammary imaging window. Nat Methods 2008; 5:1019-21; PMID:18997781; http://dx.doi.org/10.1038/nmeth.1269
30. Shan S, Sorg B, Dewhirst MW. A novel rodent mammary window of orthotopic breast cancer for intravital microscopy. Microvasc Res 2003; 65:109-17; PMID:12686168; http://dx.doi.org/10.1016/S0026-2862(02)00017-1
31. Ritsma L, Steller EJ, Ellenbroek SI, Kranenburg O, Borel Rinkes IH, van Rheenen J. Surgical implantation of an abdominal imaging window for intravital microscopy. Nat Protoc 2013; 8:583-94; PMID:23429719; http://dx.doi.org/10.1038/nprot.2013.026
32. Ritsma L, Steller EJ, Beerling E, Loomans CJ, Zomer A, Gerlach C, Vrisekoop N, Seinstra D, van Gurp L, Schäfer R, et al. Intravital microscopy through an abdominal imaging window reveals a pre-micrometastasis stage during liver metastasis. Sci Transl Med 2012; 4:ra145; PMID:23115354; http://dx.doi.org/10.1126/scitranslmed.3004394
33. Farrar MJ, Bernstein IM, Schlafer DH, Cleland TA, Fetcho JR, Schaffer CB. Chronic in vivo imaging in the mouse spinal cord using an implanted chamber. Nat Methods 2012; 9:297-302; PMID:22266542; http://dx.doi.org/10.1038/nmeth.1856
34. Fenrich KK, Weber P, Hocine M, Zalc M, Rougon G, Debarbieux F. Long-term in vivo imaging of normal and pathological mouse spinal cord with subcellular resolution using implanted glass windows. J Physiol 2012; 590:3665-75; PMID:22641787; http://dx.doi.org/10.1113/jphysiol.2012.230532
35. Looney MR, Thornton EE, Sen D, Lamm WJ, Glenny RW, Krummel MF. Stabilized imaging of immune surveillance in the mouse lung. Nat Methods 2011; 8:91-6; PMID:21151136; http://dx.doi.org/10.1038/nmeth.1543
36. Presson RG Jr., Brown MB, Fisher AJ, Sandoval RM, Dunn KW, Lorenz KS, Delp EJ, Salama P, Molitoris BA, Petrache I. Two-photon imaging within the murine thorax without respiratory and cardiac motion artifact. Am J Pathol 2011; 179:75-82; PMID:21703395; http://dx.doi.org/10.1016/j.ajpath.2011.03.048
37. Gargiulo S, Greco A, Gramanzini M, Esposito S, Affuso A, Brunetti A, Vesce G. Mice anesthesia, analgesia, and care, Part I: anesthetic considerations in preclinical research. ILAR J 2012; 53:E55-69; PMID:23382271; http://dx.doi.org/10.1093/ilar.53.1.55
38. Mostany R, Portera-Cailliau C. A craniotomy surgery procedure for chronic brain imaging. J Vis Exp 2008; 680; PMID:19066562; http://dx.doi.org/10.3791/680
39. Palmer GM, Fontanella AN, Shan S, Hanna G, Zhang G, Fraser CL, Dewhirst MW. In vivo optical molecular imaging and analysis in mice using dorsal window chamber models applied to hypoxia, vasculature and fluorescent reporters. Nat Protoc 2011; 6:1355-66; PMID:21886101; http://dx.doi.org/10.1038/nprot.2011.349
40. Hart SR, Bordes B, Hart J, Corsino D, Harmon D. Unintended perioperative hypothermia. Ochsner J 2011; 11:259-70; PMID:21960760
41. Palmer GM, Fontanella AN, Shan S, Dewhirst MW. High-resolution in vivo imaging of fluorescent proteins using window chamber models. Methods Mol Biol 2012; 872:31-50; PMID:22700402; http://dx.doi.org/10.1007/978-1-61779-797-2_3
42. Ewald AJ, Werb Z, Egeblad M. Monitoring of vital signs for long-term survival of mice under anesthesia. Cold Spring Harb Protoc 2011; 2011:t5563; PMID:21285263; http://dx.doi.org/10.1101/pdb.prot5563
43. Iida H, Ohata H, Iida M, Watanabe Y, Dohi S. Isoflurane and sevoflurane induce vasodilation of cerebral vessels via ATP-sensitive K+ channel activation. Anesthesiology 1998; 89:954-60; PMID:9778013; http://dx.doi.org/10.1097/00000542-199810000-00020
44. Cavazzuti M, Porro CA, Biral GP, Benassi C, Barbieri GC. Ketamine effects on local cerebral blood f low and metabolism in the rat. J Cereb Blood Flow Metab 1987; 7:806-11; PMID:3121648; http://dx.doi.org/10.1038/jcbfm.1987.138
45. Akata T. General anesthetics and vascular smooth muscle: direct actions of general anesthetics on cellular mechanisms regulating vascular tone. Anesthesiology 2007; 106:365-91; PMID:17264732; http://dx.doi.org/10.1097/00000542-200702000-00026
46. Zuurbier CJ, Koeman A, Houten SM, Hollmann MW, Florijn WJ. Optimizing anesthetic regimen for surgery in mice through minimization of hemodynamic, metabolic, and inflammatory perturbations. Exp Biol Med (Maywood) 2014; 239:737-46; PMID:24668552; http://dx.doi.org/10.1177/1535370214524877
47. Matsuda Y, Ohsaka K, Yamamoto H, Natsume K, Hirabayashi S, Kounoike M, Inoue M. Comparison of newly developed inhalation anesthesia system and intraperitoneal anesthesia on the hemodynamic state in mice. Biol Pharm Bull 2007; 30:1716-20; PMID:17827727; http://dx.doi.org/10.1248/bpb.30.1716
48. Jenkins DE, Oei Y, Hornig YS, Yu SF, Dusich J, Purchio T, Contag PR. Bioluminescent imaging (BLI) to improve and refine traditional murine models of tumor growth and metastasis. Clin Exp Metastasis 2003; 20:733-44; PMID:14713107; http://dx.doi.org/10.1023/B:CLIN.0000006815.49932.98
49. Sabaa N, de Franceschi L, Bonnin P, Castier Y, Malpeli G, Debbabi H, Galaup A, Maier-Redelsperger M, Vandermeersch S, Scarpa A, et al. Endothelin receptor antagonism prevents hypoxia-induced mortality and morbidity in a mouse model of sickle-cell disease. J Clin Invest 2008; 118:1924-33; PMID:18382768; http://dx.doi.org/10.1172/JCI33308
50. Xu S, Jordan EK, Li W, Yang Y, Chesnick SA, Webster HD, Brocke S, Quigley L, McFarland HF, Frank JA. In vivo three-dimensional MR microscopy of mice with chronic relapsing experimental autoimmune encephalomyelitis after treatment with insulinlike growth factor-I. AJNR Am J Neuroradiol 1998; 19:653-8; PMID:9576650
51. Perkin RM, Resnik DB. The agony of agonal respiration: is the last gasp necessary? J Med Ethics 2002; 28:164-9; PMID:12042401; http://dx.doi.org/10.1136/jme.28.3.164
52. Tranquilli W, Thurmon J, Kurt A. Lumb & Jones' Veterinary Anesthesia and Analgesia, 4th Edition. Iowa: Blackwell Publishing, 2007.
53. van Versendaal D, Rajendran R, Saiepour MH, Klooster J, Smit-Rigter L, Sommeijer JP, De Zeeuw CI, Hofer SB, Heimel JA, Levelt CN. Elimination of inhibitory synapses is a major component of adult ocular dominance plasticity. Neuron 2012; 74:374-83; PMID:22542189; http://dx.doi.org/10.1016/j.neuron.2012.03.015
54. Schwenke DO, Cragg PA. Comparison of the depressive effects of four anesthetic regimens on ventilatory and cardiovascular variables in the guinea pig. Comp Med 2004; 54:77-85; PMID:15027622
55. Adam Y, Mizrahi A. Long-term imaging reveals dynamic changes in the neuronal composition of the glomerular layer. J Neurosci 2011; 31:7967-73; PMID:21632918; http://dx.doi.org/10.1523/JNEUROSCI.0782-11.2011
56. Badie B, Schartner JM, Paul J, Bartley BA, Vorpahl J, Preston JK. Dexamethasone-induced abolition of the inflammatory response in an experimental glioma model: a flow cytometry study. J Neurosurg 2000; 93:634-9; PMID:11014542; http://dx.doi.org/10.3171/jns.2000.93.4.0634
57. Essien BE, Kotiw M. Anti-inflammatory activity of hyperimmune plasma in a lipopolysaccharidemediated rat air pouch model of inflammation. Inflammation 2012; 35:58-64; PMID:21213030; http://dx.doi.org/10.1007/s10753-010-9289-x
58. Condeelis J, Pollard JW. Macrophages: obligate partners for tumor cell migration, invasion, and metastasis. Cell 2006; 124:263-6; PMID:16439202; http://dx.doi.org/10.1016/j.cell.2006.01.007
59. Ritsma L, Vrisekoop N, van Rheenen J. In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique. Nat Commun 2013; 4:2366; PMID:23978961; http://dx.doi.org/10.1038/ncomms3366
60. de Visser KE, Coussens LM. The inflammatory tumor microenvironment and its impact on cancer development. Contrib Microbiol 2006; 13:118-37; PMID:16627962; http://dx.doi.org/10.1159/000092969
61. Hudson N, Balsitis M, Everitt S, Hawkey CJ. Angiogenesis in gastric ulcers: impaired in patients taking non-steroidal anti-inflammatory drugs. Gut 1995; 37:191-4; PMID:7557566; http://dx.doi.org/10.1136/gut.37.2.191
62. Kremers P, Duvivier J, Heusghem C. Pharmacokinetic studies of co-trimoxazole in man after single and repeated doses. J Clin Pharmacol 1974; 14:112-7; PMID:4813080; http://dx.doi.org/10.1002/j.1552-4604.1974.tb02300.x
63. Kaplan SA, Weinfeld RE, Abruzzo CW, McFaden K, Jack ML, Weissman L. Pharmacokinetic profile of trimethoprim-sulfamethoxazole in man. The Journal of infectious diseases 1973; 128:Suppl:547-55 p.
64. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966; 45:493-6; PMID:5325707
65. Cho CH, Sung HK, Kim KT, Cheon HG, Oh GT, Hong HJ, Yoo OJ, Koh GY. COMP-angiopoietin-1 promotes wound healing through enhanced angiogenesis, lymphangiogenesis, and blood flow in a diabetic mouse model. Proc Natl Acad Sci U S A 2006; 103:4946-51; PMID:16543381; http://dx.doi.org/10.1073/pnas.0506352103
66. Niinomi M. Recent research and development in titanium alloys for biomedical applications and healthcare goods. Sci Technol Adv Mater 2003; 4:445-54; http://dx.doi.org/10.1016/j.stam.2003.09.002
67. Osswald M, Winkler F. Insights into cell-to-cell and cell-to-blood-vessel communications in the brain: in vivo multiphoton microscopy. Cell Tissue Res 2013; 352:149-59; PMID:23435993; http://dx.doi.org/10.1007/s00441-013-1580-3
68. Shih AY, Mateo C, Drew PJ, Tsai PS, Kleinfeld D. A polished and reinforced thinned-skull window for long-term imaging of the mouse brain. J Vis Exp 2012; 3742; PMID:22433225; http://dx.doi.org/10.3791/3742
69. Dorand RD, Barkauskas DS, Evans TA, Petrosiute A, Huang AY. Comparison of intravital thinned skull and cranial window approaches to study CNS immunobiology in the mouse cortex. IntraVital 2014; 3:e29728; http://dx.doi.org/10.4161/intv.29728
70. Xu HT, Pan F, Yang G, Gan WB. Choice of cranial window type for in vivo imaging affects dendritic spine turnover in the cortex. Nat Neurosci 2007; 10:549-51; PMID:17417634; http://dx.doi.org/10.1038/nn1883
71. Yuan F, Salehi HA, Boucher Y, Vasthare US, Tuma RF, Jain RK. Vascular permeability and microcirculation of gliomas and mammary carcinomas transplanted in rat and mouse cranial windows. Cancer Res 1994; 54:4564-8; PMID:8062241
72. Winkler F, Kienast Y, Fuhrmann M, Von Baumgarten L, Burgold S, Mitteregger G, Kretzschmar H, Herms J. Imaging glioma cell invasion in vivo reveals mechanisms of dissemination and peritumoral angiogenesis. Glia 2009; 57:1306-15; PMID:19191326; http://dx.doi.org/10.1002/glia.20850
73. Brown E, Munn LL, Fukumura D, Jain RK. In Vivo Imaging of Tumors. Cold Spring Harb Protoc; 2010:pdb.prot5452-.
74. Moy AJ, White SM, Indrawan ES, Lotfi J, Nudelman MJ, Costantini SJ, Agarwal N, Jia W, Kelly KM, Sorg BS, et al. Wide-field functional imaging of blood flow and hemoglobin oxygen saturation in the rodent dorsal window chamber. Microvasc Res 2011; 82:199-209; PMID:21787792; http://dx.doi.org/10.1016/j.mvr.2011.07.004
75. Weigelin B. GJ B, P F. Intravital third harmonic generation microscopy of collective melanoma cell invasion: Principles of interface guidance and microvesicle dynamics. IntraVital 2012; 1:32-43; http://dx.doi.org/10.4161/intv.21223
76. A F. Guidelines for the Housing of Mice in Scientific Institutions Animal Research Review Panel 1 2012; Guideline 22.
77. Maeda A, DaCosta RS. Optimization of the dorsal skinfold window chamber model and multi-parametric characterization of tumor-associated vasculature. IntraVital 2014; 3:e27935; http://dx.doi.org/10.4161/intv.27935
78. Borgstrom P, Oh P, Czarny M, Racine B, Schnitzer JE. Co-implanting orthotopic tissue creates stroma microenvironment enhancing growth and angiogenesis of multiple tumors. F1000Res 2013; 2:129; PMID:24715954
79. Zhang SH, Zhang H, He HW, Li L, Li XQ, Zhang YP, Shao RG. Lidamycin up-regulates the expression of thymidine phosphorylase and enhances the effects of capecitabine on the growth and pulmonary metastases of murine breast carcinoma. Cancer Chemother Pharmacol 2013; 72:777-88; PMID:23975242; http://dx.doi.org/10.1007/s00280-013-2253-3
80. Shim JS, Matsui Y, Bhat S, Nacev BA, Xu J, Bhang HE, Dhara S, Han KC, Chong CR, Pomper MG, et al. Effect of nitroxoline on angiogenesis and growth of human bladder cancer. J Natl Cancer Inst 2010; 102:1855-73; PMID:21088277; http://dx.doi.org/10.1093/jnci/djq457
81. Gligorijevic B, Kedrin D, Segall JE, Condeelis J, van Rheenen J. Dendra2 photoswitching through the Mammary Imaging Window. J Vis Exp 2009; 1278; PMID:19578330; http://dx.doi.org/10.3791/1278
82. Janssen A, Beerling E, Medema R, van Rheenen J. Intravital FRET imaging of tumor cell viability and mitosis during chemotherapy. PLoS One 2013; 8:e64029; PMID:23691140; http://dx.doi.org/10.1371/journal.pone.0064029
83. Lee S, Vinegoni C, Feruglio PF, Weissleder R. Improved intravital microscopy via synchronization of respiration and holder stabilization. J Biomed Opt 2012; 17:96018-1; PMID:23085919; http://dx.doi.org/10.1117/1.JBO.17.9.096018
84. Perentes JY, McKee TD, Ley CD, Mathiew H, Dawson M, Padera TP, Munn LL, Jain RK, Boucher Y. In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts. Nat Methods 2009; 6:143-5; PMID:19151720; http://dx.doi.org/10.1038/nmeth.1295
85. Friedl P. Dynamic imaging of cellular interactions with extracellular matrix. Histochem Cell Biol 2004; 122:183-90; PMID:15258769; http://dx.doi.org/10.1007/s00418-004-0682-0
86. Campagnola PJ, Millard AC, Terasaki M, Hoppe PE, Malone CJ, Mohler WA. Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues. Biophys J 2002; 82:493-508; PMID:11751336; http://dx.doi.org/10.1016/S0006-3495(02)75414-3
87. Coffey SE, Giedt RJ, Weissleder R. Automated analysis of clonal cancer cells by intravital imaging. Intravital 2013; 2:2; PMID:24349895; http://dx.doi.org/10.4161/intv.26138
88. Weber K, Thomaschewski M, Warlich M, Volz T, Cornils K, Niebuhr B, Täger M, Lütgehetmann M, Pollok JM, Stocking C, et al. RGB marking facilitates multicolor clonal cell tracking. Nat Med 2011; 17:504-9; PMID:21441917; http://dx.doi.org/10.1038/nm.2338
89. Snippert HJ, van der Flier LG, Sato T, van Es JH, van den Born M, Kroon-Veenboer C, Barker N, Klein AM, van Rheenen J, Simons BD, et al. Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells. Cell 2010; 143:134-44; PMID:20887898; http://dx.doi.org/10.1016/j.cell.2010.09.016
90. Yuan B, Uchiyama S, Liu Y, Nguyen KT, Alexandrakis G. High-resolution imaging in a deep turbid medium based on an ultrasoundswitchable fluorescence technique. Appl Phys Lett 2012; 101:33703; PMID:22893732; http://dx.doi.org/10.1063/1.4737211
91. Pei Y, Wei MY, Cheng B, Liu Y, Xie Z, Nguyen K, Yuan B. High resolution imaging beyond the acoustic diffraction limit in deep tissue via ultrasoundswitchable NIR fluorescence. Sci Rep 2014; 4:4690; PMID:24732947; http://dx.doi.org/10.1038/srep04690
92. Flusberg BA, Nimmerjahn A, Cocker ED, Mukamel EA, Barretto RP, Ko TH, Burns LD, Jung JC, Schnitzer MJ. High-speed, miniaturized fluorescence microscopy in freely moving mice. Nat Methods 2008; 5:935-8; PMID:18836457; http://dx.doi.org/10.1038/nmeth.1256
93. Piyawattanametha W, Cocker ED, Burns LD, Barretto RP, Jung JC, Ra H, Solgaard O, Schnitzer MJ. In vivo brain imaging using a portable 2.9 g two-photon microscope based on a microelectromechanical systems scanning mirror. Opt Lett 2009; 34:2309-11; PMID:19649080; http://dx.doi.org/10.1364/OL.34.002309
94. Barretto RP, Messerschmidt B, Schnitzer MJ. In vivo fluorescence imaging with high-resolution microlenses. Nat Methods 2009; 6:511-2; PMID:19525959; http://dx.doi.org/10.1038/nmeth.1339
95. Lee S, Vinegoni C, Feruglio PF, Fexon L, Gorbatov R, Pivoravov M, Sbarbati A, Nahrendorf M, Weissleder R. Real-time in vivo imaging of the beating mouse heart at microscopic resolution. Nat Commun 2012; 3:1054; PMID:22968700; http://dx.doi.org/10.1038/ncomms2060
96. Kimura H, Hayashi K, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Kishimoto H, Bouvet M, Hoffman RM. Real-time imaging of single cancer-cell dynamics of lung metastasis. J Cell Biochem 2010; 109:58-64; PMID:19911396
97. Kobat D, Horton NG, Xu C. In vivo two-photon microscopy to 1.6-mm depth in mouse cortex. J Biomed Opt 2011; 16:106014; PMID:22029361; http://dx.doi.org/10.1117/1.3646209
98. Barretto RP, Ko TH, Jung JC, Wang TJ, Capps G, Waters AC, Ziv Y, Attardo A, Recht L, Schnitzer MJ. Time-lapse imaging of disease progression in deep brain areas using fluorescence microendoscopy. Nat Med 2011; 17:223-8; PMID:21240263; http://dx.doi.org/10.1038/nm.2292
99. Tanaka K, Morimoto Y, Toiyama Y, Matsushita K, Kawamura M, Koike Y, Okugawa Y, Inoue Y, Uchida K, Araki T, et al. In vivo time-course imaging of tumor angiogenesis in colorectal liver metastases in the same living mice using two-photon laser scanning microscopy. J Oncol 2012; 2012:265487; PMID:22131993; http://dx.doi.org/10.1155/2012/265487
100. Tanaka K, Okigami M, Toiyama Y, Morimoto Y, Matsushita K, Kawamura M, Hashimoto K, Saigusa S, Okugawa Y, Inoue Y, et al. In vivo real-time imaging of chemotherapy response on the liver metastatic tumor microenvironment using multiphoton microscopy. Oncol Rep 2012; 28:1822-30; PMID:22923070
101. Kotsuma M, Parashurama N, Smith BR, Wo J, Ito K, Gambhir SS. Nondestructive, serial in vivo imaging of a tissue-flap using a tissue adhesion barrier: Applications for IVM imaging in the mammary fat pad and lymph node. IntraVital 2012; 1:69-76; http://dx.doi.org/10.4161/intv.21769
102. Moeller BJ, Cao Y, Li CY, Dewhirst MW. Radiation activates HIF-1 to regulate vascular radiosensitivity in tumors: role of reoxygenation, free radicals, and stress granules. Cancer Cell 2004; 5:429-41; PMID:15144951; http://dx.doi.org/10.1016/S1535-6108(04)00115-1
103. Vinegoni C, Lee S, Feruglio PF, Weissleder R. Advanced Motion Compensation Methods for Intravital Optical Microscopy. IEEE J Sel Top Quantum Electron 2014; 20; PMID:24273405; http://dx.doi.org/10.1109/JSTQE.2013.2279314
104. Lee S, Vinegoni C, Sebas M, Weissleder R. Automated motion artifact removal for intravital microscopy, without a priori information. Sci Rep 2014; 4:4507; PMID:24676021; http://dx.doi.org/10.1038/srep04507