A Method for 2-Photon imaging of blood flow in the neocortex through a cranial window

Journal of Visualized Experiments

Volumn , Issue 12, 2008, Pages

  Mostany, Ricardo ^a   Portera Cailliau, Carlos ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

2 photon; Capillary; Cortex; Dextran; Fluorescein; Issue 12; Neuroscience; Red blood cell; Rhodamine; Two photon

Indexed keywords

EID: 80055117408     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/678     Document Type: Article

References (4)

1. Kleinfeld D, Mitra P.P., Helmchen F., Denk W. Fluctuations and stimulus-induced changes in blood flow observed in individual capillaries in layers 2 through 4 of rat neocortex. Proc Nat Acad Sci, USA. 95, 15741-6 (1998).
2. Pologruto, T.A., Sabatini B.L. & Svoboda, K. ScanImage: flexible software for operating laser scanning microscopes. Biomed. Eng. Online. 17, 2-13 (2003).
3. Zhang S., Murphy T.H. Imaging the impact of cortical microcirculation on synaptic structure and sensory-evoked hemodynamic responses in vivo. 5, e119 (2007).
4. Nishimura N., Schaffer C.B., Friedman B., Lyden P.D., Kleinfeld D. Penetrating arterioles are a bottleneck in the perfusion of neocortex. Proc Nat Acad Sci, USA. 104, 365-70 (2007)