In vivo two-photon microscopy to 1.6-mm depth in mouse cortex

Journal of Biomedical Optics

Volumn 16, Issue 10, 2011, Pages

  Kobat, Demirhan ^a   Horton, Nicholas G ^a   Xu, Chris ^a  

^a Cornell University   (United States)

Author keywords

Fluorescence; Imaging; Microscopy; Multiphoton processes; Scattering

Indexed keywords

FLUORESCENT DYE;

ANIMAL; ARTICLE; BRAIN CIRCULATION; BRAIN CORTEX; HISTOLOGY; LIGHT RELATED PHENOMENA; MALE; METHODOLOGY; MOUSE; MULTIPHOTON MICROSCOPY; THREE DIMENSIONAL IMAGING; VASCULARIZATION;

ANIMALS; CEREBRAL CORTEX; CEREBROVASCULAR CIRCULATION; FLUORESCENT DYES; IMAGING, THREE-DIMENSIONAL; MALE; MICE; MICROSCOPY, FLUORESCENCE, MULTIPHOTON; OPTICAL PHENOMENA;

EID: 84857687028     PISSN: 10833668     EISSN: 15602281     Source Type: Journal    
DOI: 10.1117/1.3646209     Document Type: Article

References (18)

1. F. Helmchen and W. Denk, "Deep tissue two-photon microscopy," Nat. Methods 2(12), 932-940 (2005). (Pubitemid 43108729)
2. K. Svoboda, F. Helmchen, W. Denk, and D. W. Tank, "Spread of dendritic excitation in layer 2/3 pyramidal neurons in rat barrel cortex in vivo," Nat. Neuroscience 2(1), 65-73 (1999). (Pubitemid 30488942)
3. D. Dombeck, A. Khabbaz, F. Collman, T. Adelman, and D. Tank, "Imaging large-scale neural activity with cellular resolution in awake, mobile mice," Neuron 56, 43-57 (2007). (Pubitemid 47484173)
4. R. Mostany and C. Portera-Cailliau, "Absence of large-scale dendritic plasticity of layer 5 pyramidal neurons in peri-infarct cortex," J. Neurosci. 31(5), 1734-1738 (2011).
5. T.-H. Tsai, C.-Y. Lin, H.-J. Tsai, S.-Y. Chen, S.-P. Tai, K.-H. Lin, and C.-K. Sun, "Biomolecular imaging based on far-red fluorescent protein with a high two-photon excitation action cross section," Opt. Lett. 31(7), 930-932 (2006). (Pubitemid 43837509)
6. R. M. Williams, A. Flesken-Nikitin, L. H. Ellenson, D. C. Connolly, T. C. Hamilton, A. Y. Nikitin, and W. R. Zipfel, "Startegies for highresolution imaging of epithelial ovarian cancer by laparoscopic nonlinear microscopy," Transl. Oncol. 3(3), 181-194 (2010).
7. R. Yadav, S. Mukherjee, M. Hermen, G. Tan, F. R. Maxfield, W. W. Webb, and A. K. Tewari, "Multiphoton microscopy of prostate and periprostatic neural tissue: a promising imaging technique for improving nerve-sparing prostatectomy," J. Endourol. 23(5), 861-867 (2009).
8. D. Kobat, M. E. Durst, N. Nishimura, A. W. Wong, C. B. Schaffer, and C. Xu, "Deep tissue multiphoton microscopy using longer wavelength excitation," Opt. Express 17(16), 13354-13364 (2009).
9. P. Theer, M. Hasan, and W. Denk, "Two-photon imaging to depth of 1000 μm in living brains by use of a Ti:Al2O3 regenerative amplifier," Opt. Lett. 28(12), 1022-1024 (2003).
10. A. Leray, C. Odin, E. Huguet, F. Amblard, and Y. Le Grand, "Spatially distributed two-photon excitation fluorescence in scattering media: Experiments and time-resolved Monte Carlo simulations," Opt. Commun. 272, 269-278 (2007). (Pubitemid 46108886)
11. M. Oheim, E. Beaurepaire, E. Chaigneau, J. Mertz, and S. Charpak, "Two-photon microscopy in brain tissue: Parameters influencing the imaging depth," J. Neurosci. Methods 111, 29-37 (2001). (Pubitemid 33733532)
12. P. Theer and W. Denk, "On the fundamental imaging-depth limit in twophoton microscopy," J. Opt. Soc. Am. A 23(12), 3139-3149 (2006). (Pubitemid 46023808)
13. M. Balu, T. Baldacchini, J. Carter, T. B. Krasieva, R. Zadoyan, and B. J. Tromberg, "Effect of excitation wavelength on penetration depth in nonlinear optical microscopy of turbid media," J. Biomed. Opt. 14(1), 010508 (2009).
14. V. Andresen, S. Alexander, W.-M. Heupel, M. Hirschberg, R. M. Hoffman, and P. Friedl, "Infrared multiphoton microscopy: Subcellularresolved deep tissue imaging," Curr. Opin. Biotechnol. 20, 54-62 (2009).
15. J. Herz, V. Siffrin, A. E. Hauser, A. U. Brandt, T. Leuenberger, H. Radbruch, F. Zipp, and R.A. Niesner, "Expanding two-photon intravital microscopy to the infrared by means of optical parametric oscillator," Biophys. J. 98, 715-723 (2010).
16. Q. Nguyen, P. S. Tsai, and D. Kleinfeld, "Mpscope: A versatile software suite for multiphoton microscopy," J. Neurosci. Methods 156, 351-359 (2006). (Pubitemid 44233677)
17. K. B. J. Franklin and G. Paxinos, The Mouse Brain in Stereotaxic Coordinates, 3rd Ed., Academic Press, New York (2007).
18. J. M. Girkin, S. Poland, and A. J. Wright, "Adaptive optics for deeper imaging of biological samples," Curr. Opin. Biotechnol. 20, 106-110 (2009).