Comparative metabolic analysis of CHO cell clones obtained through cell engineering, for IgG productivity, growth and cell longevity

PLoS ONE

Volumn 10, Issue 3, 2015, Pages

  Wilkens, Camila A ^a   Gerdtzen, Ziomara P ^a  

^a Dpt of Chemical Engineering   (Chile)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYL COENZYME A; CARRIER PROTEIN; CELL PROTEIN; FRUCTOSE TRANSPORTER; GLUCOSE; GLUCOSE TRANSPORTER 5; GLUTAMINE; IMMUNOGLOBULIN G; LACTIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE; PROTEIN MDH II; PROTEIN PYC2; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; UNCLASSIFIED DRUG; CARBON; FRUCTOSE;

ARTICLE; BATCH CELL CULTURE; CARBON METABOLISM; CARBON SOURCE; CELL DENSITY; CELL ENGINEERING; CELL GROWTH; CELL LONGEVITY; CELL METABOLISM; CELL PROLIFERATION; CELL VIABILITY; CELLULAR PARAMETERS; CHO CELL LINE; COMPARATIVE STUDY; CONTROLLED STUDY; FEMALE; FRUCTOSE TRANSPORTER GENE; GENE; GENETIC TRANSFECTION; GROWTH RATE; IMMUNOGLOBULIN PRODUCTION; MOLECULAR CLONING; MOLECULAR DYNAMICS; NONHUMAN; OXIDATION REDUCTION STATE; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN SECRETION; PROTEIN SYNTHESIS; THERMODYNAMICS; ANIMAL; CELL CLONE; CELL LINE; CITRIC ACID CYCLE; CRICETULUS; LONGEVITY; METABOLISM; OXIDATION REDUCTION REACTION; PHYSIOLOGY; PROCEDURES; TRANSPORT AT THE CELLULAR LEVEL;

ANIMALIA;

ANIMALS; BATCH CELL CULTURE TECHNIQUES; BIOLOGICAL TRANSPORT; CARBON; CELL ENGINEERING; CELL LINE; CELL PROLIFERATION; CHO CELLS; CITRIC ACID CYCLE; CLONE CELLS; CRICETULUS; FRUCTOSE; GLUCOSE; IMMUNOGLOBULIN G; LONGEVITY; OXIDATION-REDUCTION; THERMODYNAMICS;

EID: 84928911186     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0119053     Document Type: Article

References (37)

1. Gambhir A, Korke R, Lee J, Fu PC, Europa A, Hu WS. Analysis of cellular metabolism of hybridoma cells at distinct physiological states. J Biosci Bioeng. (2003); 95: 317-327. PMID: 16233414
2. Glacken MW, Fleischaker RJ, Sinskey AJ. Reduction of waste product excretion via nutrient control: Possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells. Biotechnol Bioeng. (1986); 28: 1376-1389. PMID: 18561227
3. Korke R, Gatti Mde L, Lau AL, Lim JW, Seow TK, Chung MC, et al. Large scale gene expression profiling of metabolic shift of mammalian cells in culture. J Biotechnol. (2004); 107: 1-17. PMID: 14687967
4. Kurano N, Leist C, Messi F, Kurano S, Fiechter A. Growth behavior of Chinese hamster ovary cells in a compact loop bioreactor. 2. Effects of medium components and waste products. J Biotechnol. (1990); 15: 113-128. PMID: 1366684
5. Glacken MW. Catabolic Control of Mammalian Cell Culture. Nat Biotechnol (1988); 6: 1041-1050.
6. Wurm FM. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat Biotechnol. (2004); 22: 1393-1398. PMID: 15529164
7. Irani N, Wirth M, van Den Heuvel J, Wagner R. Improvement of the primary metabolism of cell cultures by introducing a new cytoplasmic pyruvate carboxylase reaction. Biotechnol Bioeng. (1999); 66: 238-246. PMID: 10578094
8. Irani N, Beccaria AJ, Wagner R. Expression of recombinant cytoplasmic yeast pyruvate carboxylase for the improvement of the production of human erythropoietin by recombinant BHK-21 cells. J Biotechnol. (2002); 93: 269-282. PMID: 11755990
9. Elias CB, Carpentier E, Durocher Y, Bisson L, Wagner R, Kamen A. Improving glucose and glutamine metabolism of human HEK 293 and Trichoplusia ni insect cells engineered to express a cytosolic pyruvate carboxylase enzyme. Biotechnol Prog. (2003); 19: 90-97. PMID: 12573011
10. Fogolin MB, Wagner R, Etcheverrigaray M, Kratje R. Impact of temperature reduction and expression of yeast pyruvate carboxylase on hGM-CSF-producing CHO cells. J Biotechnol. (2004); 109: 179-191. PMID: 15063626
11. Altamirano C, Paredes C, Cairo JJ, Godia F. Improvement of CHO cell culture medium formulation: simultaneous substitution of glucose and glutamine. Biotechnol Prog. (2000); 16: 69-75. PMID: 10662492
12. Eagle H, Barban S, Levy M, Schulze HO. The utilization of carbohydrates by human cell cultures. J Biol Chem. (1958); 233: 551-558. PMID: 13575411
13. Wlaschin KF, Hu WS. Engineering cell metabolism for high-density cell culture via manipulation of sugar transport. J Biotechnol. (2007); 131: 168-176. PMID: 17662499
14. Inoue Y, Tsukamoto Y, Yamanaka M, Nakamura S, Inoue A, Nishino N, et al. Efficient production of recombinant IgG by metabolic control and co-expression with GLUT5 in a fructose-based medium. Cytotechnology. (2010); 62: 301-306. doi: 10.1007/s10616-010-9289-6 PMID: 20697810
15. Chong WP, Reddy SG, Yusufi FN, Lee DY, Wong NS, Heng CK, et al. Metabolomics-driven approach for the improvement of Chinese hamster ovary cell growth: overexpression of malate dehydrogenase II. J Biotechnol. (2010); 147: 116-121. doi: 10.1016/j.jbiotec.2010.03.018 PMID: 20363268
16. Jones ML, Seldon T, Smede M, Linville A, Chin DY, Barnard R, et al. A method for rapid, ligation-independent reformatting of recombinant monoclonal antibodies. J Immunol Methods. (2010); 354: 85-90. doi: 10.1016/j.jim.2010.02.001 PMID: 20153332
17. Wilkens CA, Altamirano C, Gerdtzen ZP. Comparative metabolic analysis of lactate for CHO cells in glucose and galactose Biotechnol Bioprocess Eng. (2011); 16.
18. Fussenegger M, Mazur X, Bailey JE. A novel cytostatic process enhances the productivity of Chinese hamster ovary cells. Biotechnol Bioeng. (1997); 55: 927-939. doi: 10.1002/(SICI)1097-0290(19970920)55:6〈927::AID-BIT10〉3.0.CO;2-4 PMID: 18636602
19. Terada S, Suzuki E, Ueda H, Makishima F. Cytokines involving gp130 in signal transduction suppressed growth of a mouse hybridoma cell line and enhanced its antibody production. Cytokine. (1996); 8: 889-894. PMID: 9050746
20. Borth N, Mattanovich D, Kunert R, Katinger H. Effect of increased expression of protein disulfide isomerase and heavy chain binding protein on antibody secretion in a recombinant CHO cell line. Biotechnol Prog. (2005); 21: 106-111. PMID: 15903247
21. Boissel JP, Lee WR, Presnell SR, Cohen FE, Bunn HF. Erythropoietin structure-function relationships. Mutant proteins that test a model of tertiary structure. J Biol Chem. (1993); 268: 15983-15993. PMID: 8340419
22. Rozwarski DA, Diederichs K, Hecht R, Boone T, Karplus PA. Refined crystal structure and mutagenesis of human granulocyte-macrophage colony-stimulating factor. Proteins. (1996); 26: 304-313. PMID: 8953651
23. Boada J, Roig T, Perez X, Gamez A, Bartrons R, Cascante M, et al. Cells overexpressing fructose-2,6-bisphosphatase showed enhanced pentose phosphate pathway flux and resistance to oxidative stress. FEBS Lett. (2000); 480: 261-264. PMID: 11034341
24. Siddiquee KAZ, Arauzo-Bravo M, Shimizu K. Metabolic flux analysis of pykF gene knockout Escherichia coli based on 13C-labeling experiments together with measurements of enzyme activities and intracellular metabolite concentrations. Appl Microbiol Biotechnol (2004); 63: 407-417. PMID: 12802531
25. Yamamoto S, Gunji W, Suzuki H, Toda H, Suda M, Jojima T, et al. Overexpression of genes encoding glycolytic enzymes in Corynebacterium glutamicum enhances glucose metabolism and alanine production under oxygen deprivation conditions. Appl Environ Microbiol (2012); 78: 4447-4457. doi: 10.1128/AEM.07998-11 PMID: 22504802
26. Lehninger AL, Nelson DL, Cox MM (2008) Lehninger principles of biochemistry. New York: W.H. Freeman. 1100 p.
27. Kerbey AL, Radcliffe PM, Randle PJ. Diabetes and the control of pyruvate dehydrogenase in rat heart mitochondria by concentration ratios of adenosine triphosphate/adenosine diphosphate, of reduced/oxidized nicotinamide-adenine dinucleotide and of acetyl-coenzyme A/coenzyme A. Biochem J. (1977); 164: 509-519. PMID: 196589
28. Kummel A, Panke S, Heinemann M. Putative regulatory sites unraveled by network-embedded thermodynamic analysis of metabolome data. Mol Syst Biol. (2006); 2: 2006 0034. PMID: 16788595
29. Schuster R, Holzhutter HG, Jacobasch G. Interrelations between glycolysis and the hexose monophosphate shunt in erythrocytes as studied on the basis of a mathematical model. Biosystems. (1988); 22: 19-36. PMID: 3191218
30. Longmore WJ, Niethe CM, McDaniel ML. Effect of CO2 concentration on intracellular pH and on glycogen synthesis from glycerol and glucose in isolated perfused rat liver. J Biol Chem. (1969); 244: 6451-6457. PMID: 5354963
31. Sies H, Noack G, Halder KH. Carbon-dioxide concentration and the distribution of monocarboxylate and H+ ions between intracellular and extracellular spaces of hemoglobin-free perfused rat liver. Eur J Biochem. (1973); 38: 247-258. PMID: 4773874
32. Williamson DH, Lund P, Krebs HA. The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver. Biochem J. (1967); 103: 514-527. PMID: 4291787
33. Zupke C, Sinskey AJ, Stephanopoulos G. Intracellular flux analysis applied to the effect of dissolved oxygen on hybridomas. Appl Microbiol Biotechnol. (1995); 44: 27-36. PMID: 8579834
34. Mooney RA, Lane MD. Control of ketogenesis and fatty-acid synthesis at the mitochondrial branchpoint for acetyl-CoA in the chick liver cell: effect of adenosine 30, 50-monophosphate. Eur J Biochem. (1982); 121: 281-287. PMID: 6277621
35. Wu F, Yang F, Vinnakota KC, Beard DA. Computer modeling of mitochondrial tricarboxylic acid cycle, oxidative phosphorylation, metabolite transport, and electrophysiology. J Biol Chem. (2007); 282: 24525-24537. PMID: 17591785
36. Robinson BH, Williams GR, Halperin ML, Leznoff CC. Factors affecting the kinetics and equilibrium of exchange reactions of the citrate-transporting system of rat liver mitochondria. J Biol Chem. (1971); 246: 5280-5286. PMID: 5094670
37. Europa AF, Gambhir A, Fu PC, Hu WS. Multiple steady states with distinct cellular metabolism in continuous culture of mammalian cells. Biotechnol Bioeng. (2000); 67: 25-34. PMID: 10581433