Two dimensional separations of human urinary protein digest using a droplet-interfaced platform

Analytica Chimica Acta

Volumn 863, Issue 1, 2015, Pages 86-94

  Ye, Linquan ^a   Wang, Xin ^a   Han, Jing ^a   Gao, Fan ^a   Xu, Lingjia ^a   Xiao, Zhiliang ^a   Bai, Peiming ^b   Wang, Qiuquan ^a   Zhang, Bo ^a  

^a XIAMEN UNIVERSITY   (China)

^b ZHONGSHAN HOSPITAL OF XIAMEN UNIVERSITY   (China)

Author keywords

Capillary electrophoresis; Liquid chromatography; Microfluidics; Multidimensional separation; Proteomics

Indexed keywords

CAPILLARY ELECTROPHORESIS; DIFFUSION IN LIQUIDS; DIFFUSION IN SOLIDS; DROPS; LIQUID CHROMATOGRAPHY; LIQUIDS; MICROFLUIDICS; MOLECULAR BIOLOGY; SEPARATION;

DROPLET MICROFLUIDICS; HIGH-RESOLUTION MAPPING; HUMAN URINE SAMPLES; MOLECULAR DIFFUSION; MULTIDIMENSIONAL SEPARATIONS; NANOFLOW LIQUID CHROMATOGRAPHIES; PROTEOMICS; TWO-DIMENSIONAL SEPARATIONS;

CHROMATOGRAPHY;

ADULT; ARTICLE; CAPILLARY CHROMATOGRAPHY; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; DROPLET MICROFLUIDICS; FRACTIONATION; HUMAN; MALE; MICROFLUIDICS; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN URINE LEVEL; PROTEOMICS; SEPARATION TECHNIQUE; TWO DIMENSIONAL NANOFLOW LIQUID CHROMATOGRAPHY; URINALYSIS; DEVICES; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; MICROFLUIDIC ANALYSIS; NANOTECHNOLOGY; PARTICLE SIZE; SURFACE PROPERTY;

PROTEIN;

ADULT; CHROMATOGRAPHY, LIQUID; ELECTROPHORESIS, CAPILLARY; HUMANS; MALE; MICROFLUIDIC ANALYTICAL TECHNIQUES; NANOTECHNOLOGY; PARTICLE SIZE; PROTEINS; SURFACE PROPERTIES; URINALYSIS;

EID: 84928893971     PISSN: 00032670     EISSN: 18734324     Source Type: Journal    
DOI: 10.1016/j.aca.2015.01.006     Document Type: Article

References (36)

1. Abbott A. And now for the proteome. Nature 2001, 409:747-748.
2. Anderson N.L., Anderson N.G. The human plasma proteome: history, character, and diagnostic prospects. Mol. Cell. Proteomics 2002, 1:845-867.
3. Separation Methods in Proteomics 2006, CRC, Press, Boca Raton. G.B. Smejkal, A. Lazareu (Eds.).
4. Giddings J.C. Two dimensional separations: concept and promise. Anal. Chem. 1984, 56:1258A-1270A.
5. Multidimensional Liquid Chromatography: Theory and Applications in Industrial Chemistry and the Life Sciences 2008, Wiley, Hoboken. S.A. Cohen, M.R. Schure (Eds.).
6. Snyder L.R., Kirkland J.J., Dolan J.W. Introduction to Modern Liquid Chromatography 2010, 35-131. Wiley, Hoboken. third ed.
7. Murphy R.E., Schure M.R., Foley J.P. Effect of sampling rate on resolution in comprehensive two-dimensional liquid chromatography. Anal. Chem. 1998, 70:1585-1594.
8. Gao M.X., Qi D.W., Zhang P., Deng C.H., Zhang X.M. Development of multidimensional liquid chromatography and application in proteomic analysis. Expert Rev. Proteomics 2010, 7:665-678.
9. Xu X., Liu K., Fan Z.H. Microscale 2D separation systems for proteomic analysis. Expert Rev. Proteomics 2012, 9:135-147.
10. Song H., Chen D.L., Ismagilov R.F. Reactions in droplets in microfluidic channels. Angew. Chem. Int. Ed. 2006, 45:7336-7356.
11. Theberge A.B., Courtois F., Schaerli Y., Fischlechner M., Abell C., Hollfelder F., Huck W.T.S. Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology. Angew. Chem. Int. Ed. 2010, 49:5846-5868.
12. Chiu D.T., Lorenz R.M., Jeffries G.D.M. Droplets for ultrasmall-volume analysis. Anal. Chem. 2009, 81:5111-5118.
13. Joensson H.N., Svahn H.A. Droplet microfluidics-a tool for single-cell analysis. Angew. Chem. Int. Ed. 2012, 51:12176-12192.
14. Xiao Z.L., Niu M.L., Zhang B. Droplet microfluidics based micro separation systems. J. Sep. Sci. 2012, 35:1284-1293.
15. Edgar J.S., Milne G., Zhao Y.Q., Pabbati C.P., Lim D.S.W., Chiu D.T. Compartmentalization of chemically separated components into droplets. Angew. Chem. Int. Ed. 2009, 48:2719-2722.
16. Niu X.Z., Zhang B., Marszalek R.T., Ces O., Edel J.B., Klug D.R., deMello A.J. Droplet-based compartmentalization of chemically separated components in two-dimensional separations. Chem. Commun. 2009, 6159-6161.
17. Theberge A.B., Whyte G., Huck W.T.S. Generation of picoliter droplets with defined contents and concentration gradients from the separation of chemical mixtures. Anal. Chem. 2010, 82:3449-3453.
18. Li Q., Pei J., Song P., Kennedy R.T. Fraction collection from capillary liquid chromatography and off-line electrospray ionization mass spectrometry using oil segmented flow. Anal. Chem. 2010, 82:5260-5267.
19. Gorbatsova J., Borissova M., Kaljurand M. Electrowetting-on-dielectric actuation of droplets with capillary electrophoretic zones for off-line mass spectrometric analysis. J. Chromatogr. A 2012, 1234:9-15.
20. Ji J., Nie L., Yang P., Liu B. Simultaneous online enrichment and identification of trace species based on microfluidic droplets. Anal. Chem. 2013, 85:9617-9622.
21. Pereira F., Niu X.Z., deMello A.J. A nano LC-MALDI mass spectrometry droplet interface for the analysis of complex protein samples. PLoS One 2013, 8:e63087.
22. Küster S.K., Pabst M., Jefimovs K., Zenobi R., Dittrich P.S. High-resolution droplet-based fractionation of nano-LC separations onto microarrays for MALDI-MS analysis. Anal. Chem. 2014, 86:4848-4855.
23. Wang X.L., Zhu Y., Fang Q. Coupling liquid chromatography/mass spectrometry detection with microfluidic droplet array for label-free enzyme inhibition assay. Analyst 2014, 139:191-197.
24. Solubility of FC-40 in water is <5 ppm and solubility of water in FC-40 is <7 ppm in weight, according to Product Information for Fluorinert Liquids, 3M, St. Paul, MN, 2003.
25. Xiao Z.L., Wang L., Liu Y., Wang Q.Q., Zhang B. A plug-and-use approach towards facile fabrication of capillary columns for high performance nanoflow liquid chromatography. J. Chromatogr. A 2014, 1325:109-114.
26. Han J., Ye L.Q., Xu L.J., Zhou Z.H., Gao F., Xiao Z.L., Wang Q.Q., Zhang B. Towards high peak capacity separations in normal pressure nanoflow liquid chromatography using meter long packed capillary columns. Anal. Chim. Acta 2014, 852:267-273.
27. Handbook of Capillary and Microchip Electrophoresis and Associated Techniques 2008, 3-74. CRC, Press, Boca Raton. J.P. Landers (Ed.).
28. Urban P.L., Goodall D.M., Bergstrom E.T., Bruce N.C. Electrophoretically mediated microanalysis of a nicotinamide adenine dinucleotide-dependent enzyme and its facile multiplexing using an active pixel sensor UV detector. J. Chromatogr. A 2007, 1162:132-140.
29. Meyer V.R. Practical High-Performance Liquid Chromatography 2010, 65. Wiley, Chichester. fifth ed.
30. A succinct introduction of contact angle measurement and its quantitative relationship with surface tension can be found at . http://www.kruss.de/services/education-theory/glossary/contact-angle/.
31. Surface Science Techniques 2013, 3-34. Springer, Berlin. G. Bracco, B. Holst (Eds.).
32. Coon J.J., Zurbig P., Dakna M., Dominiczak A.F., Decramer S., Fliser D., Frommberger M., Golovko I., Good D.M., Herget-Rosenthal S., Jankowski J., Julian B.A., Kellmann M., Kolch W., Massy Z., Novak J., Rossing K., Schanstra J.P., Schiffer E., Theodorescu D., Vanholder R., Weissinger E.M., Mischak H., Schmitt-Kopplin P. CE-MS analysis of the human urinary proteome for biomarker discovery and disease diagnostics. Proteomics Clin. Appl. 2008, 2:964-973.
33. Decramer S., de Peredo A.G., Breuil B., Mischak H., Monsarrat B., Bascands J.L., Schanstraa J.P. Urine in clinical proteomics. Mol. Cell. Proteomics 2008, 7:1850-1862.
34. Marimuthu A., O'Meally R.N., Chaerkady R., Subbannayya Y., Nanjappa V., Kumar P., Kelkar D.S., Pinto S.M., Sharma R., Renuse S., Goel R., Christopher R., Delanghe B., Cole R.N., Harsha H.C., Pandey A. A comprehensive map of the human urinary proteome. J. Proteome Res. 2011, 10:2734-2743.
35. Aebersold R., Mann M. Mass spectrometry-based proteomics. Nature 2003, 422:198-207.
36. Köcher T., Pichler P., Swart R., Mechtler K. Analysis of protein mixtures from whole-cell extracts by single-run nanoLC-MS/MS using ultralong gradients. Nat. Protoc. 2012, 7:882-890.