Heat shock protein 27 overexpression in CHO cells modulates apoptosis pathways and delays activation of caspases to improve recombinant monoclonal antibody titre in fed-batch bioreactors

Biotechnology Journal

Volumn 10, Issue 5, 2015, Pages 790-800

  Tan, Janice G L ^a,b   Lee, Yih Yean ^a   Wang, Tianhua ^a   Yap, Miranda G S ^a   Tan, Tin Wee ^b   Ng, Say Kong ^a,c  

^a AGENCY FOR SCIENCE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Apoptosis; Chinese hamster ovary (CHO); Fed batch bioreactor; Heat shock protein 27 (HSP27); Monoclonal antibody production

Indexed keywords

ANTIBODIES; BATCH CELL CULTURE; BIOCONVERSION; BIOREACTORS; CELL CULTURE; CELL DEATH; CELLS; CLONE CELLS; CLONING; CYTOLOGY; MONOCLONAL ANTIBODIES; PROTEINS; RECOMBINANT PROTEINS;

CHINESE HAMSTER OVARY; CO-IMMUNOPRECIPITATIONS; FED-BATCH BIOREACTORS; HEAT SHOCK PROTEIN; IMPROVE PERFORMANCE; MONOCLONAL ANTIBODY PRODUCTION; RECOMBINANT MONOCLONAL ANTIBODY; VOLUMETRIC PRODUCTION;

CELL ENGINEERING;

CASPASE; CASPASE 2; CASPASE 3; CASPASE 8; CASPASE 9; CYTOCHROME C; DAXX PROTEIN; HEAT SHOCK PROTEIN 27; MONOCLONAL ANTIBODY; PROTEIN KINASE B; RECOMBINANT ANTIBODY; RHESUS D ANTIGEN; RECOMBINANT PROTEIN;

ANIMAL CELL; ANTIBODY TITER; APOPTOSIS; ARTICLE; CELL COUNTING; CELL CULTURE; CELL DENSITY; CELL ENGINEERING; CELL GROWTH; CELL LEVEL; CELL VIABILITY; CELL VOLUME; CHO CELL LINE; FED BATCH REACTOR; GENETIC TRANSFECTION; GLYCOSYLATION; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; INTRACELLULAR SIGNALING; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; ANIMAL; BATCH CELL CULTURE; BIOREACTOR; BIOSYNTHESIS; BIOTECHNOLOGY; CELL PROLIFERATION; CELL SURVIVAL; CRICETULUS; DEVICES; GENETICS; METABOLISM; PROCEDURES;

CRICETULUS GRISEUS;

ANIMALS; ANTIBODIES, MONOCLONAL; APOPTOSIS; BATCH CELL CULTURE TECHNIQUES; BIOREACTORS; BIOTECHNOLOGY; CASPASES; CELL PROLIFERATION; CELL SURVIVAL; CHO CELLS; CRICETULUS; HSP27 HEAT-SHOCK PROTEINS; HUMANS; RECOMBINANT PROTEINS;

EID: 84928826243     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201400764     Document Type: Article

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