Quantitative and multiplexed DNA methylation analysis using long-read single-molecule real-time bisulfite sequencing (SMRT-BS)

BMC Genomics

Volumn 16, Issue 1, 2015, Pages

  Yang, Yao ^a   Sebra, Robert ^a   Pullman, Benjamin S ^a   Qiao, Wanqiong ^a   Peter, Inga ^a   Desnick, Robert J ^a   Geyer, C Ronald ^b   DeCoteau, John F ^b   Scott, Stuart A ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF SASKATCHEWAN   (Canada)

Author keywords

Bisulfite sequencing; CpG islands; DNA methylation; Long read sequencing; Pacific Bioscience; Single molecule real time (SMRT) sequencing; Third generation sequencing

Indexed keywords

DNA FRAGMENT; HYDROGEN SULFITE; SULFITE;

AMPLICON; ARTICLE; ARTIFACT REDUCTION; CANCER CELL LINE; COST EFFECTIVENESS ANALYSIS; CPG ISLAND; DNA DETERMINATION; DNA METHYLATION; DNA MICROARRAY; DNA SEQUENCE; GENE AMPLIFICATION; GENOME; HEMATOLOGIC MALIGNANCY; HUMAN; HUMAN CELL; MULTIPLEXED DNA METHYLATION ANALYSIS; NORMAL HUMAN; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; SEQUENCE ANALYSIS; SINGLE MOLECULE REAL TIME BISULFITE SEQUENCING; VALIDATION PROCESS; DRUG EFFECTS; GENETICS; HUMAN GENOME; PROCEDURES; TIME FACTOR; TUMOR CELL LINE;

CELL LINE, TUMOR; DNA METHYLATION; GENOME, HUMAN; HUMANS; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SULFITES; TIME FACTORS;

EID: 84928812813     PISSN: None     EISSN: 14712164     Source Type: Journal    
DOI: 10.1186/s12864-015-1572-7     Document Type: Article

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