Cloning and characterization of a new β-Glucosidase from a metagenomic library of Rumen of cattle feeding with Miscanthus sinensis

BMC Biotechnology

Volumn 14, Issue 1, 2014, Pages 1-9

  Li, Yadan ^a,b   Liu, Ning ^b,c   Yang, Hui ^a,b   Zhao, Fei ^a,b   Yu, Ye ^a,b,d   Tian, Yun ^a,b   Lu, Xiangyang ^a,b  

^a HUNAN AGRICULTURAL UNIVERSITY   (China)

^b Hunan Agricultural Bioengineering Research Institute   (China)

^c HUNAN NORMAL UNIVERSITY   (China)

^d SHANGHAI JIAO TONG UNIVERSITY SCHOOL OF MEDICINE   (China)

Author keywords

Metagenomic library; Miscanthus sinensis; Rumen; glucosidase

Indexed keywords

CELLULOSE; ENZYME ACTIVITY; LIGNIN; SACCHARIFICATION;

ENZYMATIC ACTIVITIES; GLUCOSIDASE; GLYCOSIDE HYDROLASES; METAGENOMIC LIBRARIES; MISCANTHUS SINENSIS; OPTIMAL CONDITIONS; RENEWABLE ENERGIES; RUMEN;

PLANTS (BOTANY);

BETA GLUCOSIDASE; GLUCOSE; BACTERIAL PROTEIN;

AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ANIMAL FOOD; ARTICLE; BETA GLUCOSIDASE GENE; BOVINE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME STABILITY; GENE ISOLATION; GENE LIBRARY; GENETIC CODE; GENETIC SCREENING; HYDROGEN BOND; METAGENOMICS; MISCANTHUS SINENSIS; MOLECULAR CLONING; NONHUMAN; PH MEASUREMENT; PLANT; RUMEN; TEMPERATURE SENSITIVITY; ANALYSIS; ANIMAL; BACTERIUM; CHEMISTRY; CLASSIFICATION; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; METAGENOME; MICROBIOLOGY; MOLECULAR GENETICS; PHYLOGENY; POACEAE; PROTEIN TERTIARY STRUCTURE;

AMINO ACID SEQUENCE; ANIMAL FEED; ANIMALS; BACTERIA; BACTERIAL PROTEINS; BETA-GLUCOSIDASE; CATTLE; CLONING, MOLECULAR; ENZYME STABILITY; GENE LIBRARY; KINETICS; METAGENOME; MOLECULAR SEQUENCE DATA; PHYLOGENY; POACEAE; PROTEIN STRUCTURE, TERTIARY; RUMEN;

EID: 84928793777     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-14-85     Document Type: Article

References (32)

1. Sims RE, Mabee W, Saddler JN, Taylor M: An overview of second generation biofuel technologies. Bioresour Technol 2010, 101:1570-1580.
2. Yi Z, Ruihong Z: Overview of biomass pretreatment for cellulosic ethanol production. Int J Agric Biol Eng 2009, 2:51-69.
3. Shumny VK, Veprev SG, Nechiporenko NN, Goryachkovskaya TN, Slynko NM, Kolchanov NA, Peltek SE: A new variety of Chinese silver grass (Miscanthus sinensis anderss.): a promising source of cellulose containing raw material. Russ J Genet Appl Res 2011, 1:29-32.
4. Chesson ASC, Dalgarno K, King TP: Degradtion of ioslated grass mesophyll, epidermis, and fiber cell walls in the rumen and by cellulolytic rumen bacteria in axenic culture. J Appl Bacteriol 1986, 60:327-336.
5. Lynd LR, Weimer PJ, vanZyl WH, Pretorius IS: Microbial cellulose utilization: fundamentals and biotechnology. Microbiol Mol Biol Rev 2002, 66:506-577.
6. Bokkenheuser VD, Shackleton CH, Winter J: Hydrolysis of dietary flavonoid glycosides by strains of intestinal bacteroides from humans. Biochem J 1987, 248:953-956.
7. Freer SN: Kinetic characterization of a beta-glucosidase from a yeast, Candida wickerhamii. J Biol Chem 1993, 268:9337-9342.
8. Shallom D, Shoham Y: Microbial hemicellulases. Curr Opin Microbiol 2003, 6:219-228.
9. Dahai G, Shishir PSC, Tongjun L, Spencer H, Krishne G, Phillip B, Bruce ED, Venkatesh B: Strategy for identification of novel fungal and bacterial glycosyl hydrolase hybrid mixtures that can efficiently saccharify pretreated lignocellulosic biomass. Bioenerg Res 2010, 3:67-81.
10. Saha BC, Freer SN, Bothast RJ: Production, purification, and properties of a thermostable beta-glucosidase from a color variant strain of aureobasidium pullulans. Appl Environ Microbiol 1994, 60:3774-3780.
11. Brulc JM, Antonopoulos DA, Miller ME, Wilson MK, Yannarell AC, Dinsdale EA, Edwards RE, Frank ED, Emerson JB, Wacklin P, Countinho PM, Henrissat B, Nelson KE, White BA: Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases. Proc Natl Acad Sci U S A 2009, 106:1948-1953.
12. Kristen MD, Martin A, D'haeseleer P, Julian LF, Amitha R, Philip H, Steven WS, Jean SVG, Whendee LS, Blake AS, Terry CH: Strategies for enhancing the effectiveness of metagenomic-based enzyme discovery in lignocellulolytic microbial communities. Bioenerg Res 2010, 3:146-158.
13. Banik JJ, Brady SF: Recent application of metagenomic approaches toward the discovery of antimicrobials and other bioactive small molecules. Curr Opin Microbiol 2010, 13:603-609.
14. Tuffin M, Anderson D, Heath C, Cowan DA: Metagenomic gene discovery: how far have we moved into novel sequence space? Biotechnol J 2009, 4:1671-1683.
15. Bao L, Huang Q, Chang L, Sun Q, Zhou J, Lu H: Cloning and characterization of two beta-glucosidase/xylosidase enzymes from yak rumen metagenome. Appl Biochem Biotechnol 2012, 166:72-86.
16. Jiang CJ, Chen G, Huang J, Huang Q, Jin K, Shen PH, Li JF, Wu B: A novel beta-glucosidase with lipolytic activity from a soil metagenome. Folia Microbiol (Praha) 2011, 56:563-570.
17. Wang Q, Qian C, Zhang XZ, Liu N, Yan X, Zhou Z: Characterization of a novel thermostable beta-glucosidase from a metagenomic library of termite gut. Enzyme Microb Technol 2012, 51:319-324.
18. Li Y, Hu L, Xue G, Liu H, Yang H, Zhu Y, Tian Y, Lu X: Comparison of bacterial diversity in large intestine of Xiangxi yellow cattle (Bos taurus) associated with different diet: fresh Miscanthus sinensis and mixed forage. Afr J Microbiol Res 2012, 6:5965-5974.
19. Del-Pozo MV, Fernandez-Arrojo L, Gil-Martinez J, Montesinos A, Chernikova TN, Nechitaylo TY, Waliszek A, Tortajada M, Rojas A, Huws SA, Golyshina OV, Newbold CJ, Polaina J, Ferrer M, Golyshin PN: Microbial beta-glucosidases from cow rumen metagenome enhance the saccharification of lignocellulose in combination with commercial cellulase cocktail. Biotechnol Biofuels 2012, 5:73.
20. Mrázek JTK, Avguštin G, Kopecnỳ J: Diet-dependent shifts in ruminal butyrate-producing bacteria. Folia Microbiol (Praha) 2006, 51:294-298.
21. Mariat D, Firmesse O, Levenez F, Guimaraes V, Sokol H, Dore J, Corthier G, Furet JP: The firmicutes/bacteroidetes ratio of the human microbiota changes with age. BMC Microbiol 2009, 9:123.
22. Martens EC, Koropatkin NM, Smith TJ, Gordon JI: Complex glycan catabolism by the human gut microbiota: the bacteroidetes sus-like paradigm. J Biol Chem 2009, 284:24673-24677.
23. Ferrer M, Ghazi A, Beloqui A, Vieites JM, Lopez-Cortes N, Marín-Navarro J, Nechitaylo TY, Guazzaroni ME, Polaina J, Waliczek A, Chernikova TN, Reva ON, Golyshina OV, Golyshin PN: Functional metagenomics unveils a multifunctional glycosyl hydrolase from the family 43 catalysing the breakdown of plant polymers in the calf rumen. PLoS One 2012, 7:e38134.
24. Eijsink VG, Gaseidnes S, Borchert TV, van den Burg B: Directed evolution of enzyme stability. Biomol Eng 2005, 22:21-30.
25. Cesar Mateo JMP, Gloria FL, Jose MG, Roberto FL: Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme Microb Technol 2007, 40:1451-1463.
26. Feng Y, Duan CJ, Liu L, Tang JL, Feng JX: Properties of a metagenome-derived beta-glucosidase from the contents of rabbit cecum. Biosci Biotechnol Biochem 2009, 73:1470-1473.
27. Belancic A, Gunata Z, Vallier MJ, Agosin E: Beta-glucosidase from the grape native yeast debaryomyces vanrijiae: purification, characterization, and its effect on monoterpene content of a Muscat grape juice. J Agric Food Chem 2003, 51:1453-1459.
28. Zanoelo FF, Polizeli ML, Terenzi HF, Jorge JA: Beta-glucosidase activity from the thermophilic fungus scytalidium thermophilum is stimulated by glucose and xylose. FEMS Microbiol Lett 2004, 240:137-143.
29. Fang Z, Fang W, Liu J, Hong Y, Peng H, Zhang X, Sun B, Xiao Y: Cloning and characterization of a beta-glucosidase from marine microbial metagenome with excellent glucose tolerance. J Microbiol Biotechnol 2010, 20:1351-1358.
30. Eberhart B, Cross DF, Chase LR: Beta-glucosidase system of neurospora crassa. I. Beta-glucosidase and cellulase activities of mutant and wildtype strains. J Bacteriol 1964, 87:761-770.
31. Jatinder Kaur BSC, Badhan AK, Ghatora SK, Harvinder SS: Purification and characterization of β-glucosidase from Melanocarpus sp. MTCC 3922. Electron J Biotechnol 2007, 10:260-270.
32. Bateman A, Coin L, Durbin R, Finn RD, Hollich V, Sonnhammer EL: The Pfam protein families database. Nucleic Acids Res 2004, 32:D138-D141.