Measurement of autophagy by flow cytometry

Current Protocols in Cytometry

Volumn , Issue SUPPL.68, 2014, Pages

  Warnes, Gary ^a  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

Autolysosomes; Autophagosomes; Autophagy; LC3 II; Lysosomes; Lysotracker

Indexed keywords

CHLOROQUINE; FLUORESCENT DYE; MICROTUBULE ASSOCIATED PROTEIN; MICROTUBULE ASSOCIATED PROTEIN LC3 II; POLYCLONAL ANTIBODY; PROTEIN ANTIBODY; RAPAMYCIN; UNCLASSIFIED DRUG; ANTIBODY; MAP1LC3A PROTEIN, HUMAN;

ANTIBODY LABELING; ARTICLE; AUTOLYSOSOME; AUTOPHAGY; CELL LABELING; CELL SIZE; CELL VIABILITY; CONTROLLED STUDY; FLOW CYTOMETRY; INTRACELLULAR SIGNALING; JURKAT CELL LINE; LIGHT CHAIN; PRIORITY JOURNAL; UPREGULATION; CHEMISTRY; HUMAN; K562 CELL LINE; METABOLISM; PROCEDURES;

ANTIBODIES; AUTOPHAGY; FLOW CYTOMETRY; HUMANS; JURKAT CELLS; K562 CELLS; MICROTUBULE-ASSOCIATED PROTEINS;

EID: 84928785851     PISSN: 19349297     EISSN: 19349300     Source Type: Journal    
DOI: 10.1002/0471142956.cy0945s68     Document Type: Article

References (33)

1. Ashford, T.P. and Porter, K. 1962. Cytoplasmic components in hepatic cell lysosomes. J. Cell Biol. 12:198-202.
2. Barth, S., Glick, D., and Macleod, K. Autophagy: Assays and artifacts. 2010. J. Pathol. 221:117-124.
3. Boya, P., Gonzalez-Polo, R.A, Poncet, D., Andreau, K., Vieira, H.L.A., Roumier, T., Perfettini, J.L., andKroemer G. 2003. Mitochondrial membrane permeabilization is a critical step of lysosomeinitiated apoptosis induced by hydroxychloroquine. Oncogene 22:3927-3936.
4. Boya, P., Gonzalez-Polo, R.A., Casares, N., Perfettini, J.L., Dessen, P., Larochette, N., Metivier, D., Meley, D., Souquere, S., Yoshimori, T., Pierron, G., Codogno, P., and Kroemer, G. 2005. Inhibition of macroautophagy triggers apoptosis. Mol. Cell. Biol. 25:1025-1040.
5. Byun, J.Y., Yoon, C.H., An, S., Park, I.C., Kang, C.M., Kim, M.J., and Lee, S.J. 2009. The Rac1/MKK7/JNK pathway signals upregulation of Atg5 and subsequent autophagic cell death in response to oncogenic Ras. Carcinogenesis 30:1880-1888.
6. Campbell, G.R. and Spector, S.A. 2011. Hormonally active vitamin D3 (1a, 25-dihydroxycholecalciferol) triggers autophagy in human macrophages that inhibits HIV-1 infection. J. Biol. Chem. 286:18890-18902.
7. Cao, C., Subhawong, T., Albert, J.M., Kim, K.W., Geng, L., Sekhar, K.R., Gi, Y.J., and Lu, B. 2006. Inhibition of mammalian target of rapamycin or apoptotic pathway induces autophagy and radiosensitizes PTEN null prostate cancer cells. Cancer Res. 66:10040-10047.
8. Chen, Y., McMillan-Ward, E., Kong, J., Israels, S.J, and Gibson, S. 2007. Oxidative stress induces autophagic cell death independent of apoptosis in transformed and cancer cells. Cell Death Different. 15:171-182.
9. Chikte, S., Panchal, N., and Warnes, G. 2013. Use of LysoTracker dyes: A flow cytometric study of autophagy. Cytometry A doi:10.1002/cyto.a.22333.
10. Cho, Y.S. andKwon, H. 2010. Control of autophagy with small molecules. Arch. Pharmacol. Res. 33:1881-1889.
11. Deter, R.L. and Duve, C.D. 1967. Influence of glucagon, an inducer of cellular autophagy, on some physical properties of rat liver lysosomes. J. Cell Biol. 33:437-449.
12. Geng, Y., Kohil, L., Klocke, B.J., and Roth K.A. 2010. Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent. Neuro-Oncology 12: 473-481.
13. Hailey, D.W., Rambold, A.S., Satpute-Krishnan, P., Mitra, K., Sougrat, R., Kim, P.K., and Lippencott-Schwartz, J. 2010. Mitochondria supply membranes for autophagosome biogenesis during starvation. Cell 141:656-667.
14. Hansen, T.E. and Johansen, T. 2011. Following autophagy step by step. BMC Biol. 9:1-4.
15. Kabeya, Y., Mizushima, N., Ueno, T., Yamamoto, A., Kirisako, T., Noda, T., Kominami, E., Ohsumi, Y., and Yoshimori, T. 2000. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J. 19:5720-5728.
16. Kim, I., Rodriguez-Enriquez, S., and Lemasters, J. 2007. Selective degradation of mitochondria by mitophagy. Arch. Biochem. Biophys. 462:245-253.
17. Kimura, S., Noda, T., and Yoshimori, T. 2007. Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3. Autophagy 3: 452-460.
18. Komatsu, M. and Ichimura, Y. 2010. MBSJ MCC Young Scientist Award 2009: Selective autophagy regulates various cellular functions. Genes Cells 15:923-933.
19. Mehrpour, M., Esclatine, A., Beau, I., andCodogno, P. 2010. Overview of macroautophagy regulation in mammalian cells. Cell Res. 20:748-762. Mellen, M.A., de la Rosa, E.J., and Boya P. 2009. Autophagy is not universally required for phosphatidyl-serine exposure and apoptotic cell engulfment during neural development. Autophagy 5:964-972.
20. Mellen, M.A., de la Rosa, E.J., and Boya P. 2009. Autophagy is not universally required for phosphatidyl-serine exposure and apoptotic cell engulfment during neural development. Autophagy 5:964-972
21. Paglin, S., Lee, N.Y., Nakar, C., Fitzgerlad, M., Plotkin, J., Deuel, B., Hackett, N., McMahill, M., Sphicas, E., Lampden, N., and Yahalom, J. 2005. Rapamycin-sensitive pathway regulates mitochondrial membrane potential, autophagy, and survival in irradiated MCF-7 cells. Cancer Res. 65:11061-11070.
22. Phadwal, K., Alegre-Abarrategui, J., Watson, A.S., Pike, L., Anbalagan, S., Hammond, E.M., Wade-Martins., R., McMichael, A., Klenerman, P., and Simon, A.K. 2012. A novel method for autophagy detection in primary cells: Impaired levels of macroautophagy in immunosenescent T cells. Autophagy 8:677-689.
23. Rodriguez-Enriquez, S., Kim, I., Currin, R.T., and Lemasters, J.L. 2006. Tracker dyes to probe mitochondrial autophagy (mitophagy) in rat hepatocytes. Autophagy 2:39-47.
24. Rosello, A., Warnes, G., and Meier, U.C. 2012. Cell death pathways and autophagy in the central nervous system and its involvement in neurodegeneration, immunity and CNS infection: To die or not to die-that is the question. Clin. Exp. Immunol. 168:52-57.
25. Shacka, J.J., Klocke, B.J., Shibata, M., Uchiyama, Y., Datta, G., Schmidt, R.E., and Roth, K.A. 2006. Bafilomycin A1 inhibits chloroquine-Iinduced death of cerebellar granule neurons. Mol. Pharmacol. 69:1125-1136.
26. Shintani, T. and Klionsky, D. 2004. Autophagy in health and disease: A double-edged sword. Science 306:990-995.
27. Shvets, E., Fass, E., and Elazar, Z. 2008. Utilizing flow cytometry to monitor autophagy in living mammalian cells. Autophagy 4: 621-628.
28. Thomas, S., Thurn, K.T., Biçaku, E., Marchion, D.C., and M̈unster, N. 2011. Addition of a histone deacetylase inhibitor redirects tamoxifentreated breast cancer cells into apoptosis, which is opposed by the induction of autophagy. Breast Cancer Res. Treatment 130: 437-447.
29. Tooze, S. and Yoshimori, T. 2010. The origin of the autophagosomal membrane. Nat. Cell Biol. 12:831-835.
30. Vega, F., Medeiros, L.J., Levetaki, V., Atwell, C., Cho-Vega, J.H., Tian, L., Claret, F.X., and Rassidakis, G.Z. 2006. Activation of mammalian target of rapamycin signaling pathway contributes to tumor cell survival in anaplastic lymphoma kinase-positive anaplastic large cell lymphoma. Cancer Res. 66:6589-6597.
31. Wu, Y.T., Tan, H.L., Huang, Q., Kim, Y.S., Pan, N., Ong, W.Y., Liu, Z.G., and Shen, H.M. 2008. Autophagy plays a protective role during zVADinduced necrotic cell death. Autophagy 4:457-466.
32. Xie, Z. and Klionsky, D. 2007. Autophagosomes formation: Core machinery and adaptations. Nat. Cell Biol. 9:1102-1109.
33. Yang, Z. and Klionsky, D. 2010. Eaten alive: A history of macroautophagy. Nat. Cell Biol. 12:814-822.