Codon-optimized expression and purification of truncated ORF2 protein of hepatitis E virus in Escherichia coli

Jundishapur Journal of Microbiology

Volumn 7, Issue 7, 2014, Pages

  Farshadpour, Fatemeh ^a   Taherkhani, Reza ^a   Makvandi, Manoochehr ^a   Memari, Hamid Rajabi ^b   Samarbafzadeh, Ali Reza ^a  

^a AHVAZ JUNDISHAPUR UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b SHAHID CHAMRAN UNIVERSITY OF AHVAZ   (Iran)

Author keywords

Escherichia coli; Hepatitis E virus; Purification

Indexed keywords

CAPSID PROTEIN; ISOPROPYL THIOGALACTOSIDE; ORF2 PROTEIN; UNCLASSIFIED DRUG; VIRUS PROTEIN;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; BACTERIAL CELL; BL21 CELL LINE; CELL LINE; CELL TRANSFORMATION; CODON; CONCENTRATION (PARAMETERS); CULTURE MEDIUM; DNA SEQUENCE; ESCHERICHIA COLI; EXPRESSION VECTOR; HEPATITIS E VIRUS; MOLECULAR CLONING; NONHUMAN; ORF2 GENE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; RECOMBINANT PLASMID; RESTRICTION ENZYME DIGESTION; VIRUS GENE; WESTERN BLOTTING;

EID: 84928720370     PISSN: 20083645     EISSN: 20084161     Source Type: Journal    
DOI: 10.5812/jjm.11261     Document Type: Article

References (33)

1. Yamashita T, Mori Y, Miyazaki N, Cheng RH, Yoshimura M, Unno H, et al. Biological and immunological characteristics of hepatitis E virus-like particles based on the crystal structure. Proc Natl Acad Sci U S A. 2009;106(31):12986-91.
2. Uchida T. [Cloning and sequencing of cDNA of the hepatitis E virus genome--application to diagnosis]. Nihon Rinsho. 1993;51(2):352-6.
3. Zhou YX, Lee MY, Ng JM, Chye ML, Yip WK, Zee SY, et al. A truncated hepatitis E virus ORF2 protein expressed in tobacco plastids is immunogenic in mice. World J Gastroenterol. 2006;12(2):306-12.
4. Xing L, Wang JC, Li TC, Yasutomi Y, Lara J, Khudyakov Y, et al. Spatial configuration of hepatitis E virus antigenic domain. J Virol. 2011;85(2):1117-24.
5. Zhang J, Liu CB, Li RC, Li YM, Zheng YJ, Li YP, et al. Randomizedcontrolled phase II clinical trial of a bacterially expressed recombinant hepatitis E vaccine. Vaccine. 2009;27(12):1869-74.
6. Emerson SU, Purcell RH. Recombinant vaccines for hepatitis E. Trends Mol Med. 2001;7(10):462-6.
7. Srivastava R, Aggarwal R, Jameel S, Puri P, Gupta VK, Ramesh VS, et al. Cellular immune responses in acute hepatitis E virus infection to the viral open reading frame 2 protein. Viral Immunol. 2007;20(1):56-65.
8. Zhu FC, Zhang J, Zhang XF, Zhou C, Wang ZZ, Huang SJ, et al. Efficacy and safety of a recombinant hepatitis E vaccine in healthy adults: a large-scale, randomised, double-blind placebo-controlled, phase 3 trial. Lancet. 2010;376(9744):895-902.
9. Aggarwal R, Shukla R, Jameel S, Agrawal S, Puri P, Gupta VK, et al. T-cell epitope mapping of ORF2 and ORF3 proteins of human hepatitis E virus. J Viral Hepat. 2007;14(4):283-92.
10. Huang WJ, Zhang HY, Harrison TJ, Lan HY, Huang GY, Wang YC. Immunogenicity and protective efficacy in rhesus monkeys of a recombinant ORF2 protein from hepatitis E virus genotype 4. Arch Virol. 2009;154(3):481-8.
11. Panda SK, Thakral D, Rehman S. Hepatitis E virus. Rev Med Virol. 2007;17(3):151-80.
12. Naik S, Aggarwal R, Naik SR, Dwivedi S, Talwar S, Tyagi SK, et al. Evidence for activation of cellular immune responses in patients with acute hepatitis E. Indian J Gastroenterol. 2002;21(4):149-52.
13. Zhou YH, Purcell RH, Emerson SU. A truncated ORF2 protein contains the most immunogenic site on ORF2: antibody responses to non-vaccine sequences following challenge of vaccinated and non-vaccinated macaques with hepatitis E virus. Vaccine. 2005;23(24):3157-65.
14. Emerson SU, Clemente-Casares P, Moiduddin N, Arankalle VA, Torian U, Purcell RH. Putative neutralization epitopes and broad cross-genotype neutralization of Hepatitis E virus confirmed by a quantitative cell-culture assay. J Gen Virol. 2006;87(Pt 3):697-704.
15. Tsarev SA, Tsareva TS, Emerson SU, Govindarajan S, Shapiro M, Gerin JL, et al. Successful passive and active immunization of cynomolgus monkeys against hepatitis E. Proc Natl Acad Sci U S A. 1994;91(21):10198-202.
16. Zhang M, Emerson SU, Nguyen H, Engle RE, Govindarajan S, Gerin JL, et al. Immunogenicity and protective efficacy of a vaccine prepared from 53 kDa truncated hepatitis E virus capsid protein expressed in insect cells. Vaccine. 2001;20(5-6):853-7.
17. Yang EC, Chi BR, Li X, Liu Y, Gao P, Jia P, et al. Expression and characterization of a recombinant truncated capsid protein of hepatitis E virus in Pichia pastoris. Chem Res Chin Univ. 2010;26(2):235-9.
18. Gholipour A, Moosavian M, Galehdari H, Makvandi M, Memari HR, Alvandi A. Cloning and periplasmic expression of peptidoglycan-associated lipoprotein (PAL) protein of Legionella pneumophila in Escherichia coli. Jundishapur J Microbiol. 2010;3(1):1-9.
19. Hao BC, Lan X, Xing XY, Xiang HT, Wen FQ, Hu YY, et al. Cloning and Prokaryotic Expression of cDNAs from Hepatitis E Virus Structural Gene of the SW189 Strain. Thai J Vet Med. 2013;42(2):179-84.
20. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.
21. Lhomme S, Dubois M, Abravanel F, Top S, Bertagnoli S, Guerin JL, et al. Risk of zoonotic transmission of HEV from rabbits. J Clin Virol. 2013;58(2):357-62.
22. Ruan B, Zumbika E, Wang SY, Chen Y, Ma YL, Chen YG, et al. Cloning and expression of cDNAs from hepatitis E virus structural gene. Hepatobiliary Pancreat Dis Int. 2003;2(3):387-90.
23. Li F, Riddell MA, Seow HF, Takeda N, Miyamura T, Anderson DA. Recombinant subunit ORF2.1 antigen and induction of antibody against immunodominant epitopes in the hepatitis E virus capsid protein. J Med Virol. 2000;60(4):379-86.
24. Zhao Y. J. X., ZHU Y. M., XUE F. IANG Wen-hua. expression of swine Hepatitis E virus ORF2 and development of ELISA based on the expressed protein. Acta Vet et Zootechnica Sin. 2006;37(11):1198-201.
25. QU YG, ZHU GZ, JIN NY. Prokaryotic expression and identification of recombinant swine hepatitis E virus capsid protein. Chin J Biol. 2008;3:6.
26. Dong C, Meng JH. [Expression, purification and immunogenicity of a novel hepatitis E virus-like particle]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006;22(3):339-42.
27. Liu XS, Wang YL, Zhang YG, Fang T, Pan L, Lu JL, et al. Cloning, codon-optimized expression and homology modeling of structural protein VP1 from foot and mouth disease virus. Afr J Microbiol Res. 2011;5(5):486-95.
28. Wang X, Li X, Zhang Z, Shen X, Zhong F. Codon optimization enhances secretory expression of Pseudomonas aeruginosa exotoxin A in E. coli. Protein Expr Purif. 2010;72(1):101-6.
29. Garmory HS, Brown KA, Titball RW. DNA vaccines: improving expression of antigens. Genet Vaccines Ther. 2003;1(1):2.
30. Ramakrishna L, Anand KK, Mohankumar KM, Ranga U. Codon optimization of the tat antigen of human immunodeficiency virus type 1 generates strong immune responses in mice following genetic immunization. J Virol. 2004;78(17):9174-89.
31. Saiz JC, Gonzalez MJ, Borca MV, Sobrino F, Moore DM. Identification of neutralizing antigenic sites on VP1 and VP2 of type A5 foot-and-mouth disease virus, defined by neutralization-resistant variants. J Virol. 1991;65(5):2518-24.
32. Glynou K, Ioannou PC, Christopoulos TK. One-step purification and refolding of recombinant photoprotein aequorin by immobilized metal-ion affinity chromatography. Protein Expr Purif. 2003;27(2):384-90.
33. Carson M, Johnson DH, McDonald H, Brouillette C, Delucas LJ. His-tag impact on structure. Acta Crystallogr D Biol Crystallogr. 2007;63(Pt 3):295-301.