Cloning and expression of cDNAs from hepatitis E virus structural gene

Hepatobiliary and Pancreatic Diseases International

Volumn 2, Issue 3, 2003, Pages 387-390

  Ruan, Bing ^a   Zumbika, Edward ^a   Wang, Shu Ying ^b   Chen, Yong ^c   Ma, Yi Lin ^a   Chen, Ya Gang ^a   Liu, Ke Zhou ^a  

^a ZHEJIANG UNIVERSITY SCHOOL OF MEDICINE   (China)

^b HANGZHOU FIRST PEOPLE'S HOSPITAL   (China)

^c INSTITUTE OF MATERIA MEDICA   (China)

Author keywords

Cloning; Hepatitis E virus; Open reading frame; Polymerase chain reaction; Western blot

Indexed keywords

COMPLEMENTARY DNA; DNA BASE; DNA FRAGMENT; HEPATITIS VACCINE; RECOMBINANT ANTIGEN; RECOMBINANT PROTEIN; STRUCTURAL PROTEIN; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE; VIRUS FUSION PROTEIN; VIRUS VECTOR;

ANALYTICAL EQUIPMENT; ARTICLE; BLOOD ANALYSIS; CHINA; CONTROLLED STUDY; DILUTION; ENZYME LINKED IMMUNOSORBENT ASSAY; EPIDEMIC; ESCHERICHIA COLI; GENE INSERTION; HEPATITIS E; HEPATITIS E VIRUS; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; OPEN READING FRAME; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; RECOMBINANT PLASMID; SEQUENCE ANALYSIS; STRUCTURAL GENE; STRUCTURE ANALYSIS; VIRUS ISOLATION; WESTERN BLOTTING;

ANIMALS; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, VIRAL; HEPATITIS E VIRUS; MACACA MULATTA; OPEN READING FRAMES; PLASMIDS; RECOMBINANT FUSION PROTEINS;

EID: 0041911144     PISSN: 14993872     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Emerson SU, Purcell RH. Hepatitis E virus. Rev Med Virol 2003;13:145-154.
2. Engle RE, Yu C, Emerson SU, et al. Hepatitis E virus (HEV) capsid antigens derived from viruses of human and swine origin are equally efficient for detecting anti-HEV by enzyme immunoassay. J Clin Microbiol 2002;40:4576-4580.
3. Li F, Torresi J, Stephen A, et al. Amino-terminal epitopes are exposed when full-length open reading frame 2 of hepatitis E virus is expressed in Escherichia coli, but carboxy-terminal epitopes are masked. J Med Virol 1997;52:289-300.
4. Anderson DA, Li F, Riddell MA, et al. ELISA for IgG-class antibody to hepatitis E virus based on a highly conserved, conformational epitope expressed in Eschericia coli. J Virol Methods 1999; 81:131-142.
5. Sehgal D, Malik PS, Jameel S. Purification and diagnostic utility of a recombinant hepatitis E virus capsid protein expressed in insect larvae. Protein Expr Purif 2003;27:27-34.
6. Li TC, Zhang J, Shinzawa H, et al. Empty virus-like partical-based enzyme-linked immunosorbent assay for antibodies to hepatitis E virus. J Med Virol 2000;62:327-333.
7. Jameel S, Zafrullah M, Ozdener MH, et al. Expression in animal cells and characterization of the hepatitis E virus structrul proteins. J Virol 1996;70:207-216.
8. Tam AW, Smith MM, Guerra ME, et al. Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome. Virology 1991 ; 185: 120-131.
9. Huang CC, Nguyen D, Fernandez J, et al. Molecular cloning and sequencing of the Mexico isolate of hepatitis E virus (HEV). Virology 1992;191:550-558.
10. Aye TT, Uchida T, Ma XZ, et al. Complete nucleotide sequence of a hepatitis E virus isolated from the Xinjiang epidemic (1986 - 1988) of China. Nucleic Acids Res 1992;20:3512.
11. Li F, Zhuang H, Kolivas S, et al. Persistent and transient antibody responses to hepatitis E virus detected by western immunoblot using open reading frame 2 and 3 and glutathione S-transferase fusion proteins. J Clin Microbiol 1994;32:2060-2066.
12. Reyes GR, Purdy MA, Kim JP, et al. Isolation of a cDNA from the virus responsible for enterically transmitted non-A, non-B hepatitis. Science 1990; 247: 1335-1339.
13. Ruan B, Zhuang H, Ma YL, et al. Partial nucleotide sequencing of hepatitis E virus isolated from a sporadic case in Zhejiang. Chin J Microbiol Immunol 1998;18:165-167.
14. He J, Tam AW, Yarbough PO, et al. Expression and diagnostic utility of hepatitis E virus putative structural proteins expressed in insect cells. J Clin Microbiol 1993;31:2167-2173.