Stabilization of dengue virus polymerase in de novo initiation assay provides advantages for compound screening

Antiviral Research

Volumn 119, Issue , 2015, Pages 36-46

  Niyomrattanakit, Pornwaratt ^a,d   Wan, Kah Fei ^a   Chung, Ka Yan ^a,b   Abas, Siti Nurdiana ^a   Seh, Cheah Chen ^a   Dong, Hongping ^a   Lim, Chin Chin ^a   Chao, Alexander Theodore ^a   Lee, Chang Bok ^a   Nilar, Shahul ^a   Lescar, Julien ^b,c   Shi, Pei Yong ^a   Beer, David ^a   Lim, Siew Pheng ^a  

^a NOVARTIS INSTITUTE FOR TROPICAL DISEASES   (Singapore)

^b NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

^c PITIÉ SALPÊTRIÈRE HOSPITAL   (France)

^d Maveta Company Limited   (Thailand)

Author keywords

Activity; Assay; Compounds; De novo initiation; Dengue virus NS5 protein; Elongation; Inhibition; Keywords Flavivirus; Kinetics; RNA dependent; RNA polymerase

Indexed keywords

ALKALINE PHOSPHATASE; COBALT; CYSTEINE; DIVALENT CATION; INORGANIC SALT; MAGNESIUM ION; MANGANESE; NONSTRUCTURAL PROTEIN 5; VIRUS RNA; ZINC ION; ANTIVIRUS AGENT; APOENZYME; ENZYME INHIBITOR; NS5 PROTEIN, DENGUE VIRUS; RNA DIRECTED RNA POLYMERASE; VIRAL PROTEIN;

ARTICLE; ASSAY; BINDING AFFINITY; DENGUE VIRUS; DENGUE VIRUS 1; DENGUE VIRUS 2; DENGUE VIRUS 3; DENGUE VIRUS 4; ENZYME ACTIVITY; ENZYME KINETICS; FLUORESCENCE ANALYSIS; GEL FILTRATION; GENOME SIZE; IN VITRO STUDY; KINETIC PARAMETERS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN STABILITY; REACTION TIME; RNA BINDING; SCREENING TEST; SIGNAL NOISE RATIO; ANTAGONISTS AND INHIBITORS; DRUG EFFECTS; ENZYME STABILITY; ENZYMOLOGY; GENETIC TRANSCRIPTION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MICROBIAL SENSITIVITY TEST;

DENGUE VIRUS; FLAVIVIRUS;

ANTIVIRAL AGENTS; APOENZYMES; CYSTEINE; DENGUE VIRUS; ENZYME INHIBITORS; ENZYME STABILITY; HUMANS; KINETICS; MICROBIAL SENSITIVITY TESTS; RNA REPLICASE; RNA, VIRAL; TRANSCRIPTION, GENETIC; VIRAL NONSTRUCTURAL PROTEINS;

EID: 84928550645     PISSN: 01663542     EISSN: 18729096     Source Type: Journal    
DOI: 10.1016/j.antiviral.2015.04.007     Document Type: Article

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