Analysis of peptides and protein digests by reversed phase high performance liquid chromatography-electrospray ionisation mass spectrometry using neutral pH elution conditions

Analytica Chimica Acta

Volumn 872, Issue , 2015, Pages 84-94

  Yang, Yuanzhong ^a   Boysen, Reinhard I ^a   Chowdhury, Jamil ^a   Alam, Asif ^a   Hearn, Milton T W ^a  

^a MONASH UNIVERSITY   (Australia)

Author keywords

Chromatographic efficiency; Electrospray ionisation mass spectrometry; Mobile phase pH; Peptide identification; Reversed phase liquid chromatography; Selectivity

Indexed keywords

CATALYST SELECTIVITY; ELECTROSPRAY IONIZATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH PRESSURE LIQUID CHROMATOGRAPHY; LIQUIDS; MASS SPECTROMETRY; PEPTIDES; POSITIVE IONS; SEPARATION; SILICA;

CHROMATOGRAPHIC SELECTIVITY; ELECTROSPRAY IONISATION MASS SPECTROMETRIES; GRADIENT ELUTION CONDITIONS; IDENTIFICATION OF PROTEINS; MOBILE PHASE; PEPTIDE IDENTIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; REVERSED PHASE LIQUID-CHROMATOGRAPHY;

DRUG PRODUCTS;

ACETONITRILE; ALPHA 2 MACROGLOBULIN PRECURSOR; ANTITHROMBIN III PRECURSOR; APOLIPOPROTEIN A I PRECURSOR; APOLIPOPROTEIN A II PRECURSOR; APOLIPOPROTEIN A IV PRECURSOR; APOLIPOPROTEIN B 100 PRECURSOR; BETA 2 GLYCOPROTEIN 1 PRECURSOR; CERULOPLASMIN PRECURSOR; COMPLEMENT C4 B PRECURSOR; COMPLEMENT COMPONENT C3; COMPLEMENT FACTOR B PRECURSOR; COMPLEMENT FACTOR H PRECURSOR; FIBRINOGEN ALPHA CHAIN PRECURSOR; FIBRINOGEN BETA CHAIN PRECURSOR; FIBRINOGEN GAMMA CHAIN PRECURSOR; FIBRONECTIN PRECURSOR; GELSOLIN PRECURSOR; HEMOPEXIN PRECURSOR; INTER ALPHA TRYPSIN INHIBITOR HEAVY CHAIN H1 PRECURSOR; INTER ALPHA TRYPSIN INHIBITOR HEAVY CHAIN H2 PRECURSOR; INTER ALPHA TRYPSIN INHIBITOR HEAVY CHAIN H4 PRECURSOR; METHANOL; PEPTIDE; PLASMINOGEN PRECURSOR; PROTEIN; PROTEIN PRECURSOR; PROTHROMBIN PRECURSOR; SILANOL; UNCLASSIFIED DRUG; VITAMIN D BINDING PROTEIN PRECURSOR; BOVINE SERUM ALBUMIN; PEPTIDE FRAGMENT; PLASMA PROTEIN; TRYPSIN;

AMINO ACID SEQUENCE; ARTICLE; CATION EXCHANGE; CONCENTRATION RESPONSE; ELECTROSPRAY MASS SPECTROMETRY; ELUTION; HUMAN; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PROTEIN INTERACTION; PROTEOMICS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SEPARATION TECHNIQUE; TANDEM MASS SPECTROMETRY; ANIMAL; BOVINE; CHEMISTRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLISM;

ANIMALS; BLOOD PROTEINS; CATTLE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HUMANS; HYDROGEN-ION CONCENTRATION; PEPTIDE FRAGMENTS; SERUM ALBUMIN, BOVINE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TRYPSIN;

EID: 84928205117     PISSN: 00032670     EISSN: 18734324     Source Type: Journal    
DOI: 10.1016/j.aca.2015.02.055     Document Type: Article

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