A replicative plasmid vector allows efficient complementation of pathogenic Leptospira strains

Applied and Environmental Microbiology

Volumn 81, Issue 9, 2015, Pages 3176-3181

  Pappas, Christopher J ^a,b   Benaroudj, Nadia ^a   Picardeau, Mathieu ^a  

^a INSTITUT PASTEUR   (France)

^b MANHATTANVILLE COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENE EXPRESSION; GENES; MUTAGENESIS; PATHOGENS; VECTORS;

GENETIC MANIPULATIONS; GENOMIC ISLANDS; HOMOLOGOUS RECOMBINATION; PATHOGENIC STRAINS; PLASMID VECTORS; SUICIDE VECTORS; TRANSPOSON MUTAGENESIS; ZOONOTIC DISEASE;

DNA;

DISEASE VECTOR; GENOMICS; LEPTOSPIROSIS; MUTAGENICITY; PATHOGENICITY; PLASMID; PROTEIN; RECOMBINATION; SAPROTROPHY;

LEPTOSPIRA; LEPTOSPIRA INTERROGANS;

BACTERIAL DNA;

CHEMISTRY; DNA SEQUENCE; GENE VECTOR; GENETIC COMPLEMENTATION; GENETICS; GENOMIC INSTABILITY; LEPTOSPIRA; MICROBIAL GENETICS; MOLECULAR BIOLOGY; MOLECULAR GENETICS; PLASMID; PROCEDURES;

DNA, BACTERIAL; GENETIC COMPLEMENTATION TEST; GENETIC VECTORS; GENETICS, MICROBIAL; GENOMIC INSTABILITY; LEPTOSPIRA; MOLECULAR BIOLOGY; MOLECULAR SEQUENCE DATA; PLASMIDS; SEQUENCE ANALYSIS, DNA;

EID: 84927800810     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00173-15     Document Type: Article

References (18)

1. Ko AI, Goarant C, Picardeau M. 2009. Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen. Nat Rev Microbiol 7:736-747. http://dx.doi.org/10.1038/nrmicro2208.
2. Picardeau M. 2015. Genomics, proteomics, and genetics of Leptospira. Springer-Verlag, Berlin, Germany.
3. Girons IS, Bourhy P, Ottone C, Picardeau M, Yelton D, Hendrix RW, Glaser P, Charon N. 2000. The LE1 bacteriophage replicates as a plasmid within Leptospira biflexa: construction of an L. biflexa-Escherichia coli shuttle vector. J Bacteriol 182:5700-5705. http://dx.doi.org/10.1128/JB.182.20.5700-5705.2000.
4. Picardeau M. 2008. Conjugative transfer between Escherichia coli and Leptospira spp. as a new genetic tool. Appl Environ Microbiol 74:319-322. http://dx.doi.org/10.1128/AEM.02172-07.
5. Bauby H, Saint Girons I, Picardeau M. 2003. Construction and complementation of the first auxotrophic mutant in the spirochaete Leptospira meyeri. Microbiology 149:689-693. http://dx.doi.org/10.1099/mic.0.26065-0.
6. Poggi D, Oliveira de Giuseppe P, Picardeau M. 2010. Antibiotic resistance markers for genetic manipulations of Leptospira spp. Appl Environ Microbiol 76:4882-4885. http://dx.doi.org/10.1128/AEM.00775-10.
7. Picardeau M, Bulach DM, Bouchier C, Zuerner RL, Zidane N, Wilson PJ, Creno S, Kuczek ES, Bommezzadri S, Davis JC, McGrath A, Johnson MJ, Boursaux-Eude C, Seemann T, Rouy Z, Coppel RL, Rood JI, Lajus A, Davies JK, Médigue C, Adler B. 2008. Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis. PLoS One 3:e1607. http://dx.doi.org/10.1371/journal.pone.0001607.
8. Bourhy P, Salaün L, Lajus A, Médigue C, Boursaux-Eude C, Picardeau M. 2007. A genomic island of the pathogen Leptospira interrogans serovar Lai can excise from its chromosome. Infect Immun 75:677-683. http://dx.doi.org/10.1128/IAI.01067-06.
9. Bourhy P, Collet L, Brisse S, Picardeau M. 2014. Leptospira mayottensis sp. nov., a pathogenic Leptospira species isolated from humans. Int J Syst Evol Microbiol 64:4061-4067. http://dx.doi.org/10.1099/ijs.0.066597-0.
10. Lo M, Murray GL, Khoo CA, Haake DA, Zuerner RL, Adler B. 2010. Transcriptional response of Leptospira interrogans to iron limitation and characterization of a PerR homolog. Infect Immun 78:4850-4859. http://dx.doi.org/10.1128/IAI.00435-10.
11. Ellinghausen HC, McCullough WG. 1965. Nutrition of Leptospira pomona and growth of 13 other serotypes: fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80. Am J Vet Res 26:45-51.
12. Johnson RC, Harris VG. 1967. Differentiation of pathogenic and saprophytic leptospires. J Bacteriol 94:27-31.
13. Demarre G, Guerout AM, Matsumoto-Mashimo C, Rowe-Magnus DA, Marliere P, Mazel D. 2005. A new family of mobilizable suicide plasmids based on broad host range R388 plasmid (IncW) and RP4 plasmid (Inc-Palpha) conjugative machineries and their cognate Escherichia coli host strains. Res Microbiol 156:245-255. http://dx.doi.org/10.1016/j.resmic.2004.09.007.
14. Zuerner RL. 1991. Physical mapping of chromosomal and plasmid DNA comprising the genome of Leptospira interrogans. Nucleic Acids Res 19: 4857-4860. http://dx.doi.org/10.1093/nar/19.18.4857.
15. Zuerner RL, Herrmann JL, Saint Girons I. 1993. Comparison of genetic maps for two Leptospira interrogans serovars provides evidence for two chromosomes and intraspecies heterogeneity. J Bacteriol 175: 5445-5451.
16. Zhu WN, Huang LL, Zeng LB, Zhuang XR, Chen CY, Wang YZ, Qin JH, Zhu YZ, Guo XK. 2014. Isolation and characterization of two novel plasmids from pathogenic Leptospira interrogans serogroup Canicola serovar Canicola strain Gui44. PLoS Negl Trop Dis 8:e3103. http://dx.doi.org/10.1371/journal.pntd.0003103.
17. Bourhy P, Collet L, Lernout T, Zinini F, Hartskeerl RA, van der Linden H, Thiberge JM, Diancourt L, Brisse S, Giry C, Pettinelli, FPM. 2012. Human Leptospira isolates circulating in Mayotte (Indian Ocean) have unique serological and molecular features. J Clin Microbiol 50:307-311. http://dx.doi.org/10.1128/JCM.05931-11.
18. Vallenet D, Belda E, Calteau A, Cruveiller S, Engelen S, Lajus A, Le Fèvre F, Longin C, Mornico D, Roche D, Rouy Z, Salvignol G, Scarpelli C, Thil Smith AA, Weiman M, Médigue C. 2013. MicroScope-an integrated microbial resource for the curation and comparative analysis of genomic and metabolic data. Nucleic Acids Res 41:D636-D647. http://dx.doi.org/10.1093/nar/gks1194.