Construction and complementation of the first auxotrophic mutant in the spirochaete Leptospira meyeri

Microbiology

Volumn 149, Issue 3, 2003, Pages 689-693

  Bauby, Hélène ^a   Saint Girons, Isabelle ^a   Picardeau, Mathieu ^a  

^a INSTITUT PASTEUR   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ANTHRANILIC ACID; CHORISMIC ACID; GENE PRODUCT; GLUTAMINE; KANAMYCIN; SPECTINOMYCIN; TRYPTOPHAN;

AMINO ACID SYNTHESIS; ANTIBIOTIC RESISTANCE; ARTICLE; AUXOTROPHIC MUTANT; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIUM MUTANT; CONTROLLED STUDY; CROSSING OVER; CULTURE MEDIUM; ESCHERICHIA COLI; GENE CASSETTE; GENE DISRUPTION; GENETIC COMPLEMENTATION; HOMOLOGOUS RECOMBINATION; LEPTOSPIRA; LEPTOSPIRA MEYERI; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; SHUTTLE VECTOR; SPIROCHETE;

ANTHRANILATE SYNTHASE; ANTHRANILIC ACIDS; BASE SEQUENCE; DRUG RESISTANCE, BACTERIAL; ESCHERICHIA COLI; GENETIC COMPLEMENTATION TEST; GENETIC VECTORS; KANAMYCIN; LEPTOSPIRA; MOLECULAR SEQUENCE DATA; MUTATION; NITROGENOUS GROUP TRANSFERASES; RECOMBINATION, GENETIC; TRYPTOPHAN;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LEPTOSPIRA MEYERI; SPIROCHAETA; SPIROCHAETALES;

EID: 0037352557     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.26065-0     Document Type: Article

References (25)

1. Baril, C., Richaud, C., Fournie, E., Baranton, G. & Saint Girons, I. (1992). Cloning of dapD, aroD and asd of Leptospira interrogans serovar icterohaemorrhagiae, and nucleotide sequence of the asd gene. J Gen Microbiol 138, 47-53.
2. Belfaiza, J., Martel, A., Margarita, D. & Saint Girons, I. (1998). Direct sulfhydrylation for methionine biosynthesis in Leptospira meyeri. J Bacteriol 180, 250-255.
3. Bono, J. L., Elias, A. F., Kupko, J. J., Stevenson, B., Tilly, K. & Rosa, P. (2000). Efficient targeted mutagenesis in Borrelia burgdorferi. J Bacteriol 182, 2445-2452.
4. Charon, N. W., Russell, C., Johnson, C. & Peterson, D. (1974). Amino acid biosynthesis in the spirochete Leptospira: evidence for a novel pathway of isoleucine biosynthesis. J Bacteriol 117, 203-211.
5. Chary, V. K., Amaya, E. I. & Piggot, P. J. (1997). Neomycin- and spectinomycin-resistance replacement vectors for Bacillus subtilis. FEMS Microbiol Lett 153, 135-139.
6. Chi, B., Limberger, R. J. & Kuramitsu, H. K. (2002). Complementation of a Treponema denticola flgE mutant with a novel coumermycin A1-resistant T. denticola shuttle vector system. Infect Immun 70, 2233-2237.
7. Ellinghausen, H. C. & McCullough, W. G. (1965). Nutrition of Leptospira pomona and growth of 13 other serotypes: fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80. Am J Vet Res 26, 45-51.
8. Fraser, C. M., Casjens, S., Huang, W. M. & 35 other authors (1997). Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi. Nature 390, 580-586.
9. Fraser, C. M., Norris, S. J., Weinstock, C. M. & 30 other authors (1998). Complete genome sequence of Treponema pallidum, the syphilis spirochete. Science 281, 375-388.
10. Hardham, J. M. & Rosey, E. L. (2000). Antibiotic selective markers and spirochete genetics. In The Spirochetes: Molecular and Cellular Biology, pp. 101-110. Edited by M. H. Saier & J. Garcia-Lara. Wymondham, UK: Horizon Scientific Press.
11. Johnson, R. C. & Harris, V. G. (1967). Differentiation of pathogenic and saprophytic leptospires. J Bacteriol 94, 27-31.
12. Mintz, C. S., Chen, J. & Shuman, H. A. (1988). Isolation and characterization of auxotrophic mutants of Legionella pneumophila that fail to multiply in human monocytes. Infect Immun 56, 1449-1455.
13. Murphy, E., Huwyler, L. & de Freire Bastos Mdo, C. (1985). Transposon Tn554: complete nucleotide sequence and isolation of transposition-defective and antibiotic-sensitive mutants. EMBO J 4, 3357-3365.
14. Picardeau, M., Brenot, A. & Saint Girons, I. (2001). First evidence for gene replacement in Leptospira spp. Inactivation of L. biflexa flaB results in non-motile mutants deficient in endoflagella. Mol Microbiol 40, 189-199.
15. Pittard, A. J. (1996). Biosynthesis of the aromatic amino acids. In Escherichia coli and Salmonella: Cellular and Molecular Biology, pp. 458-484. Edited by F. C. Neidhart and others. Washington, DC: American Society for Microbiology.
16. Richaud, C., Margarita, D., Baranton, G. & Saint Girons, I. (1990). Cloning of genes required for amino acid biosynthesis from Leptospira interrogans serovar icterohaemorrhagiae. J Gen Microbiol 136, 651-656.
17. Saint Girons, I., Bourhy, P., Ottone, C., Picardeau, M., Yelton, D., Hendrix, R. W., Glaser, P. & Charon, N. (2000). The LE1 bacteriophage replicates as a plasmid within Leptospira biflexa: construction of an L. biflexa-Escherichia coli shuttle vector. J Bacteriol 182, 5700-5705.
18. Sartakova, M. L., Dobrikova, E. Y. & Cabello, F. C. (2001a). Constructing of Borrelia burgdorferi pGK12 cloning vector derivatives expressing kanamycin and spectinomycin resistance genes. Abstract D-164, 101st General Meeting of the American Society for Microbiology.
19. Sartakova, M. L., Dobrikova, E. Y., Motaleb, M. A., Godfrey, H. P., Charon, N. W. & Cabello, F. C. (2001b). Complementation of a nonmotile flaB mutant of Borrelia burgdorferi by chromosomal integration of a plasmid containing a wild-type flaB allele. J Bacteriol 183, 6558-6564.
20. Simmons, C. P., Hodgson, A. L. M. & Strugnell, R. A. (1997). Attenuation and vaccine potential of aroQ mutants of Corynebacterium pseudotuberculosis. Infect Immun 65, 3048-3056.
21. Smith, D. A., Parish, T., Stoker, N. G. & Bancroft, G. J. (2001). Characterization of auxotrophic mutants of Mycobacterium tuberculosis and their potential as vaccine candidate. Infect Immun 69, 1142-1150.
22. Tchamedeu Kameni, A. P., Couture-Tosi, E., Saint-Girons, I. & Picardeau, M. (2002). Inactivation of the spirochete recA gene results in a mutant with low viability and irregular nucleoid morphology. J Bacteriol 184, 452-458.
23. Tilly, K., Elias, A. F., Bono, J. L., Stewart, P. E. & Rosa, P. (2001). DNA exchange and insertional inactivation in spirochetes. In The Spirochetes: Molecular and Cellular Biology, pp. 111-122. Edited by M. H. Saier & J. Garcia-Lara. Wymondham, UK: Horizon Scientific Press.
24. Yelton, D. B. & Cohen, R. A. (1986). Analysis of cloned DNA from Leptospira biflexa serovar patoc which complements a deletion of the Escherichia coli trpE gene. J Bacteriol 165, 41-46.
25. Yelton, D. B. & Pang, S. L. (1989). Identification and nucleotide sequence of the Leptospira biflexa serovar patoc trpE and trpG genes. J Bacteriol 171, 2083-2089.