Conjugative transfer between Escherichia coli and Leptospira spp. as a new genetic tool

Applied and Environmental Microbiology

Volumn 74, Issue 1, 2008, Pages 319-322

  Picardeau, Mathieu ^a  

^a INSTITUT PASTEUR   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; DNA; ENZYMES; ESCHERICHIA COLI; MUTAGENESIS; PATHOGENS;

LEPTOSPIRA SPP.; LEPTOSPIRAL PATHOGENESIS; PATHOGENIC STRAINS; PLASMIDS;

GENETIC ENGINEERING;

DEOXYRIBONUCLEASE I;

COLIFORM BACTERIUM; DNA; GENETIC ANALYSIS; MUTATION; PLASMID;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CONJUGATION; CELL CONTACT; DNA TRANSFER; ESCHERICHIA COLI; HIMAR1 GENE; LEPTOSPIRA; LEPTOSPIRA BIFLEXA; LEPTOSPIRA INTERROGANS; LEPTOSPIROSIS; MUTANT; NONHUMAN; PATHOGENESIS; PLASMID; RELIABILITY; SAPROTROPH; TRANSPOSON;

CONJUGATION, GENETIC; DNA TRANSPOSABLE ELEMENTS; ESCHERICHIA COLI; GENETICS, MICROBIAL; LEPTOSPIRA; PLASMIDS; RECOMBINATION, GENETIC;

ESCHERICHIA COLI; LEPTOSPIRA; LEPTOSPIRA BIFLEXA; LEPTOSPIRA INTERROGANS;

EID: 37849041601     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02172-07     Document Type: Article

References (20)

1. Bourhy, P., L. Frangeul, E. Couve, P. Glaser, I. Saint Girons, and M. Picardeau. 2005. Complete nucleotide sequence of the LE1 prophage from the spirochete Leptospira biflexa and characterization of its replication and partition functions. J. Bacteriol. 187:3931-3940.
2. Bourhy, P., H. Louvel, I. Saint Girons, and M. Picardeau. 2005. Random insertional mutagenesis of Leptospira interrogans, the agent of leptospirosis, using a mariner transposon. J. Bacteriol. 187:3255-3258.
3. Demarre, G., A. M. Guerout, C. Matsumoto-Mashimo, D. A. Rowe-Magnus, P. Marliere, and D. Mazel. 2005. A new family of mobilizable suicide plasmids based on broad host range R388 plasmid (IncW) and RP4 plasmid (IncPα) conjugative machineries and their cognate Escherichia coli host strains. Res. Microbiol. 156:245-255.
4. Ellinghausen, H. C., and W. G. McCullough. 1965. Nutrition of Leptospira pomona and growth of 13 other serotypes: fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80. Am. J. Vet. Res. 26:45-51.
5. Fraser, C. M., S. J. Norris, C. M. Weinstock, O. White, G. G. Sutton, R. Dodson, M. Gwinn, E. K. Hickey, R. Clayton, K. A. Ketchum, E. Sodergren, J. M. Hardham, M. P. McLeod, S. Salzberg, J. Peterson, H. Khalak, D. Richardson, J. K. Howell, M. Chidambaram, T. Utterback, L. McDonald, P. Artiach, C. Bowman, M. D. Cotton, C. Fujii, et al. 1998. Complete genome sequence of Treponema pallidum, the syphilis spirochete. Science 281:375-388.
6. Heinemann, J. A., and G. F. Sprague. 1989. Bacterial conjugative plasmids mobilize DNA transfer between bacteria and yeast. Nature 340:205-209.
7. Holloway, B. W., and M. H. Richmond. 1973. R-factors used for genetic studies in strains of Pseudomonas aeruginosa and their origin. Genet. Res. 21:103-105.
8. Johnson, R. C., and V. G. Harris. 1967. Differentiation of pathogenic and saprophytic leptospires. J. Bacteriol. 94:27-31.
9. Lampe, D. J., B. J. Akerley, E. J. Rubin, J. J. Mekalanos, and H. M. Robertson. 1999. Hyperactive transposase mutants of the Himar1 mariner transposon. Proc. Natl. Acad. Sci. USA 96:11428-11433.
10. Le Roux, F., J. Binesse, D. Saulnier, and D. Mazel. 2007. Construction of a Vibrio splendidus mutant lacking the metalloprotease gene vsm by use of a novel counterselectable suicide vector. Appl. Environ. Microbiol. 73:777-784.
11. Louvel, H., I. Saint Girons, and M. Picardeau. 2005. Isolation and characterization of FecA- and FeoB-mediated iron acquisition systems of the spirochete Leptospira biflexa by random insertional mutagenesis. J. Bacteriol. 187:3249-3254.
12. Luzhetskyy, A., M. Fedoryshyn, O. Gromyko, B. Ostash, Y. Rebets, A. Bechthold, and V. Fedorenko. 2006. IncP plasmids are most effective in mediating conjugation between Escherichia coli and streptomycetes. Genetika 42:595-601.
13. Lyras, D., and J. I. Rood. 1998. Conjugative transfer of RP4-oriT shuttle vectors from Escherichia coli to Clostridium perfringens. Plasmid 39:160-164.
14. Pansegrau, W., E. Lanka, P. T. Barth, D. H. Figurski, D. G. Guiney, D. Haas, D. R. Helinski, H. Schwab, V. A. Stanisich, and C. M. Thomas. 1994. Complete nucleotide sequence of Birmingham IncP alpha plasmids. Compilation and comparative analysis. J. Mol. Biol. 239:623-663.
15. Picardeau, M., A. Brenot, and I. Saint Girons. 2001. First evidence for gene replacement in Leptospira spp. Inactivation of L. biflexa flaB results in non-motile mutants deficient in endoflagella. Mol. Microbiol. 40:189-199.
16. Prod'hom, G., B. Lagier, V. Pelicic, A. J. Hance, B. Gicquel, and C. Guilhot. 1998. A reliable amplification technique for the characterization of genomic DNA sequences flanking insertion sequences. FEMS Microbiol. Lett. 158: 75-81.
17. Saint Girons, I., P. Bourhy, C. Ottone, M. Picardeau, D. Yelton, R. W. Hendrix, P. Glaser, and N. Charon. 2000. The LE1 bacteriophage replicates as a plasmid within Leptospira biflexa: construction of an L. biflexa-Escherichia coli shuttle vector. J. Bacteriol. 182:5700-5705.
18. Saint Girons, I., D. Margarita, P. Amouriaux, and G. Baranton. 1990. First isolation of bacteriophages for a spirochaete: potential genetic tools for Leptospira. Res. Microbiol. 141:1131-1138.
19. Samuels, A. L., E. Lanka, and J. E. Davies. 2000. Conjugative junctions in RP4-mediated mating of Escherichia coli. J. Bacteriol. 182:2709-2715.
20. Waters, V. L. 2001. Conjugation between bacterial and mammalian cells. Nat. Genet. 29:375-376.