How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments

Biomolecular Detection and Quantification

Volumn 3, Issue , 2015, Pages 9-16

  Svec, David ^a,c   Tichopad, Ales ^b   Novosadova, Vendula ^a,c   Pfaffl, Michael W ^d   Kubista, Mikael ^a,c  

^a INSTITUTE OF BIOTECHNOLOGY   (Czech Republic)

^b CHARLES UNIVERSITY   (Czech Republic)

^c TATAA Biocenter   (Sweden)

^d TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Amplification efficiency; Dilution series; QPCR; QPCR assay validation; Real time quantitative PCR; Standard curve

Indexed keywords

COMPLEMENTARY DNA;

ANALYSIS OF COVARIANCE; ANALYTICAL PARAMETERS; ARTICLE; CALIBRATION; DILUTION; GENE EXPRESSION; GENE ISOLATION; MOUSE; NONHUMAN; PHYSICAL PHENOMENA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PRODUCTIVITY; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STANDARD CURVE METHOD;

EID: 84927722380     PISSN: None     EISSN: 22147535     Source Type: Journal    
DOI: 10.1016/j.bdq.2015.01.005     Document Type: Article

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