CyProQuant-PCR: A real time RT-PCR technique for profiling human cytokines, based on external RNA standards, readily automatable for clinical use

BMC Immunology

Volumn 6, Issue , 2005, Pages

  Boeuf, Philippe ^a   Vigan Womas, Inès ^a   Jublot, Delphine ^a   Loizon, Séverine ^a   Barale, Jean Christophe ^a   Akanmori, Bartholomew Dicky ^b   Mercereau Puijalon, Odile ^a   Behr, Charlotte ^a  

^a INSTITUT PASTEUR   (France)

^b University of Ghana   (Ghana)

Author keywords

[No Author keywords available]

Indexed keywords

BETA 2 MICROGLOBULIN; CURVED DNA; CYTOKINE; GAMMA INTERFERON; INTERLEUKIN 10; INTERLEUKIN 12P40; INTERLEUKIN 13; INTERLEUKIN 15; INTERLEUKIN 18; INTERLEUKIN 1BETA; INTERLEUKIN 4; LIPOPOLYSACCHARIDE; MESSENGER RNA; PHYTOHEMAGGLUTININ; PRIMER RNA; PROLYLLEUCYLGLYCINAMIDE; TRANSFORMING GROWTH FACTOR BETA1; TUMOR NECROSIS FACTOR ALPHA; DNA; MACROPHAGE MIGRATION INHIBITION FACTOR; TGFB1 PROTEIN, HUMAN; TRANSFORMING GROWTH FACTOR BETA;

ANALYTIC METHOD; ANALYTICAL EQUIPMENT; ARTICLE; AUTOMATION; BACTERIAL STRAIN; CHILD; CLINICAL ARTICLE; CLINICAL STUDY; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME INDUCTION; EXPERIMENTATION; GENE AMPLIFICATION; GENE EXPRESSION PROFILING; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; IN VITRO STUDY; INTERMETHOD COMPARISON; MALARIA FALCIPARUM; MEASUREMENT; NUCLEOTIDE SEQUENCE; PERIPHERAL BLOOD MONONUCLEAR CELL; PROCESS DESIGN; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STANDARDIZATION; STAPHYLOCOCCUS AUREUS; SYMPTOMATOLOGY; VALIDATION PROCESS; ADULT; BIOSYNTHESIS; CELL CULTURE; COMPARATIVE STUDY; DRUG EFFECT; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; METHODOLOGY; STANDARD; VALIDATION STUDY;

ADULT; AUTOMATION; CELLS, CULTURED; CHILD; CYTOKINES; DNA; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; HUMANS; LIPOPOLYSACCHARIDES; MACROPHAGE MIGRATION-INHIBITORY FACTORS; MALARIA, FALCIPARUM; REFERENCE STANDARDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TRANSFORMING GROWTH FACTOR BETA; TRANSFORMING GROWTH FACTOR BETA1; TUMOR NECROSIS FACTOR-ALPHA;

EID: 26444583976     PISSN: 14712172     EISSN: 14712172     Source Type: Journal    
DOI: 10.1186/1471-2172-6-5     Document Type: Article

References (25)

1. Aggarwal BB, Puri RK, eds: Human cytokines: their role in Disease and Therapy. Cambridge, MA: Blackwell Science; 1995.
2. Dinarello CA: Role of pro-inflammatory and anti-inflammatory cytokines are mediators in the pathogenesis of septic shock. Chest 1997, 112:321S-329S.
3. Carson RT, Vignali TAA: Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods 1999, 227:41-52.
4. Gogos CA, Drosou E, Bassaris HP, Skoutelis A: Pro-versus antiinflammatory cytokine profile in patients with severe sepsis: a marker for prognosis and future therapeutic options. J Infect Dis 2000, 181:176-180.
5. Zegarska J, Paczek L, Pawlowska M, Podrzucki W, Rowinski W, Malanowski P, Wszola M, Mroz A: Quantitative gene expression of TGF-β1, TNF-α, IL-1β and IL-6 in the renal artery wall of chronically rejected renal allografts. Transplant Proc 2002, 34:3176-3179.
6. Salmeri FM, Sofo V, Ando FG, Vastola MB, Crosca S, Bitto A, Gaeta M, Girbino G: Imbalance of serum cytokine network in sarcoid patients: index of sarcoidosis relapse. Sarcoidosis Vasc Diffuse Lung Dis 2003, 20:53-61.
7. Semnani RT, Liu AY, Sabzevari H, Kubofcik J, Zhou J, Gilden JK, Nutman TB: Brugia malayi microfilariae induce cell death in human dendritic cells, inhibit their ability to make IL12 and IL10 and reduce their capacity to activate CD4+ T cells. J Immunol 2003, 171:1950-1960.
8. Zimmermann AK, Simon P, Seeburger J, Hoffmann J, Ziemer G, Aebert H, Wendel HP: Cytokine gene expression in monocytes of patients undergoing cardiopulmonary bypass surgery evaluated by real-time PCR. J Cell Mol Med 2003, 7:146-156.
9. Overbergh L, Giulietti A, Valckx D, Decallonne B, Bouillon R, Mathieu C: The use of real-time reverse transciptase PCR for the quantification of cytokine gene expression. J Biomol Tech 2003, 14:33-43.
10. Rajeevan MS, Ranamukhaarachchi DG, Vernon SD, Unger ER: Use of real-time quantitative PCR to validate the results of cDNA array and differential display PCR technologies. Methods 2001, 25:443-451.
11. Nomura I, Gao B, Boguniewicz M, Darst MA, Travers JB, Leung DY: Distinct patterns of gene expression in the skin lesions of atopic dermatitis and psoriasis: a gene microarrays analysis. J Allergy Clin Immunol 2003, 112:1195-1202.
12. Ramos-Payan R, Medina-Aguilar M, Estrada-Parra S, Gonzalez-y-Merchand JA, Favilla-Castillo L, Monroy-Ostria A, Estrada-Garcia CE: Quantification of cytokine gene expression using an economical real-time PCR polymerase reaction method based on SYBRr Green I. Scand J Immunol 2003, 57:439-445.
13. Radonic A, Thulke S, Mackay IM, La d O, Siegert W, Nitsche A: Guideline to reference gene selection for quantitative realtime PCR. Biochem Biophys Res Commun 2004, 313:856-862.
14. Bustin SA: Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 2000, 25:169-173.
15. Stordeur P, Poulin LF, Cracium L, Zhou L, Schandené L, de Lavareille A, Goriely S, Goldman M: Cytokine mRNA quantification by real-time PCR. J Immunol Methods 2002, 259:55-64.
16. Ginzinger DG: Gene quantification using real-time quantitative PCR : An emerging technology hits the mainstream. Exp Hematol 2002, 30:503-512.
17. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F: Accurate normalization of real time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 2002, 3:34.I-34.II.
18. Pachot A, Blond J-L, Mougin B, Miossec P: Peptidylpropyl isomerase B (PPIB): a suitable reference gene for mRNA quantification in peripheral whole blood. J Biotechnol 2004, 114:121-124.
19. Calandra T, Froidevaux C, Martin C, Roger T: Macrophage migratory inhibitory factor and host innate immune defenses against bacterial sepsis. J Infect Dis 2003:S385-90.
20. Marino JH, Cook P, Miller KS: Accurate and statistically verified quantification of relative mRNA abundances using SYBR Green I and real-time RT-PCR. J Immunol Methods 2003, 283:291-306.
21. Meis JE, Chen F: In vitro synthesis of 2′-fluoro-modified RNA transcripts that are completely resistant to RNase A digestion using the DuraScribe T7 transcription kit. EPICENTRE forum 2002, 9:10-11.
22. Heidenreich O, Eckstein F: Hammerhead ribozyme-mediated clivage of the long terminal repeat RNA of human immunodeficiency virus type I. J Biol Chem 1992, 267:1904-1909.
23. Housseau F, Lindsey KR, Oberholtzer SD, Gonzales MI, Boutin P, Moorthy AK, Shankara S, Roberts BL, Topalian SL: Quantitative real-time RT-PCR as a method for monitoring T lymphocyte reactivity to full-length tyrosinase protein in vaccinated melanoma patients. J Immunol Methods 2002, 266:87-103.
24. Omer FM, Kurtzhals JAL, Riley EM: Maintaining the immunological balance in parasitic infections : a role for TGF-β? Parasitol Today 2000, 16:18-23.
25. Kurtzhals JAL, Adabayeri V, Quarm-Goka B, Akanmori BD, Oliver-Commey JO, Nkrumah FK, Behr C, Hviid L: Low plasma concentrations of interleukin 10 in severe malarial anaemia compared to cerebral and uncomplicated malaria. Lancet 1998, 351:1768-1772.