Structural and catalytic effects of proline substitution and surface loop deletion in the extended active site of human carbonic anhydrase II

FEBS Journal

Volumn 282, Issue 8, 2015, Pages 1445-1457

  Boone, Christopher D ^a   Rasi, Valerio ^a   Tu, Chingkuang ^a   McKenna, Robert ^a  

^a UNIVERSITY OF FLORIDA   (United States)

Author keywords

bioengineering; carbonic anhydrase; differential scanning calorimetry; protein crystallography; protein thermal stability

Indexed keywords

CARBONATE DEHYDRATASE II; PROLINE;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIA; CATALYSIS; CHEMICAL MODIFICATION; CONTROLLED STUDY; DIFFERENTIAL SCANNING CALORIMETRY; ENZYME ACTIVE SITE; ENZYME ISOLATION; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STRUCTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTON TRANSPORT; SULFURIHYDROGENIBIUM YELLOWSTONENSE; SURFACE LOOP DELETION; THERMOSTABILITY; X RAY CRYSTALLOGRAPHY; CHEMICAL STRUCTURE; CHEMISTRY; GENE DELETION; GENETICS; HUMAN; KINETICS; METABOLISM; MUTATION; PROTEIN CONFORMATION; SITE DIRECTED MUTAGENESIS; TEMPERATURE;

BACTERIA (MICROORGANISMS); SULFURIHYDROGENIBIUM YELLOWSTONENSE;

AMINO ACID SUBSTITUTION; CALORIMETRY, DIFFERENTIAL SCANNING; CARBONIC ANHYDRASE II; CATALYSIS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; HUMANS; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROLINE; PROTEIN CONFORMATION; SEQUENCE DELETION; TEMPERATURE;

EID: 84927626008     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/febs.13232     Document Type: Article

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