Nano-scale liquid chromatography coupled to tandem mass spectrometry using the multiple reaction monitoring mode based quantitative platform for analyzing multiple enzymes associated with central metabolic pathways of Saccharomyces cerevisiae using ultra fast mass spectrometry

Journal of Bioscience and Bioengineering

Volumn 119, Issue 1, 2015, Pages 117-120

  Matsuda, Fumio ^a,b   Ogura, Tairo ^c   Tomita, Atsumi ^a   Hirano, Ichiro ^c   Shimizu, Hiroshi ^a  

^a OSAKA UNIVERSITY   (Japan)

^b Elements Chemistry Laboratory   (Japan)

^c SHIMADZU CORPORATION   (Japan)

Author keywords

Central carbon metabolism; Enzyme expression; Quantitative proteomics; Saccharomyces cerevisiae; Ultra fast mass spectrometry

Indexed keywords

ENZYMES; MASS SPECTROMETRY; METABOLISM; NANOTECHNOLOGY; PEPTIDES; PROTEOMICS; YEAST;

CENTRAL CARBON METABOLISMS; LIQUID CHROMATOGRAPHY COUPLED TO TANDEM MASS SPECTROMETRY; METABOLIC PATHWAYS; MULTIPLE-REACTION MONITORING MODES; NANO LIQUID CHROMATOGRAPHY; QUANTITATIVE PROTEOMICS; RELATIVE ABUNDANCE; ULTRA-FAST;

LIQUID CHROMATOGRAPHY;

FUNGAL ENZYME; PEPTIDE DERIVATIVE; PROTEOME; TRYPSIN; PEPTIDE; SACCHAROMYCES CEREVISIAE PROTEIN;

ANALYTIC METHOD; ARTICLE; ENZYME ANALYSIS; FUNGAL METABOLISM; FUNGAL STRAIN; LIQUID CHROMATOGRAPHY; MONITORING; NANO SCALE LIQUID CHROMATOGRAPHY; NONHUMAN; QUANTITATIVE ANALYSIS; SACCHAROMYCES CEREVISIAE; TANDEM MASS SPECTROMETRY; ULTRA FAST MASS SPECTROMETRY; CHEMISTRY; ENZYMOLOGY; MASS SPECTROMETRY; METABOLISM; PROCEDURES; PROTEOMICS;

SACCHAROMYCES CEREVISIAE;

CHROMATOGRAPHY, LIQUID; MASS SPECTROMETRY; METABOLIC NETWORKS AND PATHWAYS; PEPTIDES; PROTEOME; PROTEOMICS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TANDEM MASS SPECTROMETRY; TRYPSIN;

EID: 84927173928     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2014.06.010     Document Type: Article

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