High-throughput generation of selected reaction-monitoring assays for proteins and proteomes

Nature Methods

Volumn 7, Issue 1, 2010, Pages 43-46

  Picotti, Paola ^a   Rinner, Oliver ^a,b   Stallmach, Robert ^a   Dautel, Franziska ^c   Farrah, Terry ^d   Domon, Bruno ^a   Wenschuh, Holger ^e   Aebersold, Ruedi ^a,d,f,g  

^a ETH ZURICH   (Switzerland)

^b Biognosys AG   (Switzerland)

^c HELMHOLTZ CENTRE FOR ENVIRONMENTAL RESEARCH UFZ   (Germany)

^d INSTITUTE FOR SYSTEMS BIOLOGY   (United States)

^e JPT Peptide Technologies GmbH   (Germany)

^f Competence Center for Systems Physiology and Metabolic Diseases ^*  (Switzerland)

^g UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL PROTEIN; PHOSPHATASE; PHOSPHOTRANSFERASE; SYNTHETIC PEPTIDE; PEPTIDE LIBRARY; PROTEIN; PROTEIN KINASE; PROTEOME;

ARTICLE; BIOTECHNOLOGY; CHROMATOGRAPHY; GEL ELECTROPHORESIS; HIGH THROUGHPUT SCREENING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEOMICS; SACCHAROMYCES CEREVISIAE; SELECTED REACTION MONITORING; SENSITIVITY AND SPECIFICITY; TANDEM MASS SPECTROMETRY; VALIDATION PROCESS; BIOASSAY; ENZYMOLOGY; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; PROTEIN DATABASE; REPRODUCIBILITY;

SACCHAROMYCES CEREVISIAE;

BIOLOGICAL ASSAY; DATABASES, PROTEIN; HIGH-THROUGHPUT SCREENING ASSAYS; MASS SPECTROMETRY; PEPTIDE LIBRARY; PHOSPHORIC MONOESTER HYDROLASES; PROTEIN KINASES; PROTEINS; PROTEOME; REPRODUCIBILITY OF RESULTS; SACCHAROMYCES CEREVISIAE;

EID: 74049131716     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth.1408     Document Type: Article

References (16)

1. Picotti, P., Bodenmiller, B., Mueller, L.N., Domon, B. & Aebersold, R. Cell 138, 795-806 (2009).
2. Picotti, P., Aebersold, R. & Domon, B. Mol. Cell. Proteomies 6, 1589-1598 (2007).
3. Lange, V., Picotti, P., Domon, B. & Aebersold, R. Mol. Syst. Biol. 4, 1-14 (2008).
4. Stahl-Zeng, J. et al. Mol. Cell. Proteomies 6, 1809-1817 (2007).
5. Anderson, L. & Hunter, C.L. Mol. Cell. Proteomies 5, 573-588 (2006).
6. Addona, T.A. et al. Nat. Biotechnol. 27, 633-641 (2009).
7. Lange, V. et al. Mol. Cell. Proteomies 7, 1489-1500 (2008).
8. Picotti, P. et al. Nat. Methods 5, 913-914 (2008).
9. Kuster, B., Schirle, M., Mallick, P. & Aebersold, R. Nat. Rev. Mol. Cell Biol. 6, 577-583 (2005).
10. Deutsch, E.W., Lam, H. & Aebersold, R. EMBO Rep. 9, 429-434 (2008).
11. Mallick, P. et al. Nat. Biotechnol. 25, 125-131 (2007).
12. Frank, R. & Overwin, H. Methods Mol. Biol. 66, 149-169 (1996).
13. Wenschuh, H. et al. Biopolymers 55, 188-206 (2000).
14. Ghaemmaghami, S. et al. Nature 425, 737-741 (2003).
15. de Godoy, L.M. et al. Nature 455, 1251-1254 (2008).
16. Elias, J.E. & Gygi, S.P. Nat. Methods 4, 207-214 (2007).