Importance of adhesins in virulence of Paracoccidioides spp.

Frontiers in Microbiology

Volumn 6, Issue MAR, 2015, Pages

  de Oliveira, Haroldo Cesar ^a   da Silva, Julhiany de Fátima ^a   Scorzoni, Liliana ^a   Marcos, Caroline Maria ^a   Rossi, Suelen Andréia ^a   de Paula e Silva, Ana Carolina Alves ^a   Assato, Patrícia Akemi ^a   da Silva, Rosângela Aparecida Moraes ^a   Fusco Almeida, Ana Marisa ^a   Mendes Giannini, Maria José Soares ^a  

^a SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

Author keywords

Adhesins; Adhesion; Paracoccidioides spp.; Virulence

Indexed keywords

ADHESIN; COLLAGEN TYPE 1; COLLAGEN TYPE 4; ENOLASE; FIBRONECTIN; LAMININ; PROTEIN 14 3 3;

ADULT; ANIMAL EXPERIMENT; ARTICLE; CELL VIABILITY; CONTROLLED STUDY; CORRELATION COEFFICIENT; DOWN REGULATION; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; FUNGAL VIRULENCE; GALLERIA MELLONELLA; GENE EXPRESSION; GENETIC HETEROGENEITY; HOST PATHOGEN INTERACTION; HUMAN; HUMAN CELL; LARVA; LUNG ALVEOLUS CELL; MALE; MICROBIAL ADHESION; MOUSE; NONHUMAN; PARACOCCIDIOIDES; PARACOCCIDIOIDES BRASILIENSIS; PARACOCCIDIOIDES LUTZII; REAL TIME POLYMERASE CHAIN REACTION; SURVIVAL; UPREGULATION;

FUNGI; GALLERIA MELLONELLA; MUS; PARACOCCIDIOIDES; PARACOCCIDIOIDES BRASILIENSIS;

EID: 84927132985     PISSN: None     EISSN: 1664302X     Source Type: Journal    
DOI: 10.3389/fmicb.2015.00303     Document Type: Article

References (77)

1. Q. Al Abdallah, S.I. Choe, P. Campoli, S. Baptista, F.N. Gravelat, M.J. Lee, and D.C. Sheppard (2012). A conserved C-terminal domain of the Aspergillus fumigatus developmental regulator MedA is required for nuclear localization, adhesion and virulence. PLoS One 7, e49959.
2. P.F. Andreotti, J.L. Monteiro da Silva, A.M. Bailão, C.M. Soares, G. Benard, C.P. Soares, and M.J. Mendes-Giannini (2005). Isolation and partial characterization of a 30 kDa adhesin from Paracoccidioides brasiliensis. Microbes Infect 7, 875-81.
3. E. Bagagli, S.M. Bosco, R.C. Theodoro, and M. Franco (2006). Phylogenetic and evolutionary aspects of Paracoccidioides brasiliensis reveal a long coexistence with animal hosts that explain several biological features of the pathogen. Infect Genet Evol 6, 344-51.
4. A.M. Bailão, A. Schrank, C.L. Borges, V. Dutra, E.E. Walquíria Inês Molinari-Madlum, M.S. Soares Felipe, M.J. Soares Mendes-Giannini, W.S. Martins, M. Pereira, and C. Maria de Almeida Soares (2006a). Differential gene expression by Paracoccidioides brasiliensis in host interaction conditions: representational difference analysis identifies candidate genes associated with fungal pathogenesis. Microbes Infect 8, 2686-97.
5. A.M. Bailão, A. Schrank, C.L. Borges, V. Dutra, E.E. Walquíria Inês Molinari-Madlum, M.S. Soares Felipe, M.J. Soares Mendes-Giannini, W.S. Martins, M. Pereira, and C. Maria de Almeida Soares (2006b). Differential gene expression by Paracoccidioides brasiliensis in host interaction conditions: representational difference analysis identifies candidate genes associated with fungal pathogenesis. Microbes Infect 8, 2686-97.
6. A.M. Bailão, A. Shrank, C.L. Borges, J.A. Parente, V. Dutra, M.S. Felipe, R.B. Fiúza, M. Pereira, and C.M. de Almeida Soares (2007). The transcriptional profile of Paracoccidioides brasiliensis yeast cells is influenced by human plasma. FEMS Immunol Med Microbiol 51, 43-57.
7. M.S. Barbosa, S.N. Báo, P.F. Andreotti, F.P. de Faria, M.S. Felipe, L. dos Santos Feitosa, M.J. Mendes-Giannini, and C.M. Soares (2006). Glyceraldehyde-3-phosphate dehydrogenase of Paracoccidioides brasiliensis is a cell surface protein involved in fungal adhesion to extracellular matrix proteins and interaction with cells. Infect Immun 74, 382-9.
8. J. Batista, Z.P. de Camargo, G.F. Fernandes, A.P. Vicentini, C.J. Fontes, and R.C. Hahn (2010). Is the geographical origin of a Paracoccidioides brasiliensis isolate important for antigen production for regional diagnosis of paracoccidioidomycosis? Mycoses 53, 176-80.
9. D. Bergin, E.P. Reeves, J. Renwick, F.B. Wientjes, and K. Kavanagh (2005). Superoxide production in Galleria mellonella hemocytes: identification of proteins homologous to the NADPH oxidase complex of human neutrophils. Infect Immun 73, 4161-70.
10. S. Bernardino, A. Pina, M. Felonato, T.A. Costa, E. Frank de Araújo, C. Feriotti, S.B. Bazan, A.C. Keller, K.R. Leite, and V.L. Calich (2013). TNF-a and CD8+ T cells mediate the beneficial effects of nitric oxide synthase-2 deficiency in pulmonary paracoccidioidomycosis. PLoS Negl Trop Dis 7, e2325.
11. W.e.A. Brito, T.C. Rezende, A.F. Parente, C.A. Ricart, M.V. Sousa, S.N. Báo, and C.M. Soares (2011). Identification, characterization and regulation studies of the aconitase of Paracoccidioides brasiliensis. Fungal Biol 115, 697-707.
12. V.L. Calich, C.A. Vaz, and E. Burger (1998). Immunity to Paracoccidioides brasiliensis infection. Res Immunol 149, 407-17; discussion 499-500.
13. L.L. Carrero, G. Niño-Vega, M.M. Teixeira, M.J. Carvalho, C.M. Soares, M. Pereira, R.S. Jesuino, J.G. McEwen, L. Mendoza, J.W. Taylor, M.S. Felipe, and G. San-Blas (2008). New Paracoccidioides brasiliensis isolate reveals unexpected genomic variability in this human pathogen. Fungal Genet Biol 45, 605-12.
14. A. Casadevall, and L.A. Pirofski (1999). Host-pathogen interactions: redefining the basic concepts of virulence and pathogenicity. Infect Immun 67, 3703-13.
15. O.L. Champion, I.A. Cooper, S.L. James, D. Ford, A. Karlyshev, B.W. Wren, M. Duffield, P.C. Oyston, and R.W. Titball (2009). Galleria mellonella as an alternative infection model for Yersinia pseudotuberculosis. Microbiology 155, 1516-22.
16. S.M. Cook, and J.D. McArthur (2013). Developing Galleria mellonella as a model host for human pathogens. Virulence 4, 350-3.
17. T.A. Costa, S.B. Bazan, C. Feriotti, E.F. Araújo, ê. Bassi, F.V. Loures, and V.L. Calich (2013). In pulmonary paracoccidioidomycosis IL-10 deficiency leads to increased immunity and regressive infection without enhancing tissue pathology. PLoS Negl Trop Dis 7, e2512.
18. J.e.F. da Silva, H.C. de Oliveira, C.M. Marcos, R.A. da Silva, T.A. da Costa, V.L. Calich, A.M. Almeida, and M.J. Mendes-Giannini (2013). Paracoccidoides brasiliensis 30 kDa Adhesin: Identification as a 14-3-3 Protein, Cloning and Subcellular Localization in Infection Models. PLoS One 8, e62533.
19. B.R. da Silva Neto, J. de Fátima da Silva, M.J. Mendes-Giannini, H.L. Lenzi, C.M. de Almeida Soares, and M. Pereira (2009). The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesin. BMC Microbiol 9, 272.
20. P.W. de Groot, O. Bader, A.D. de Boer, M. Weig, and N. Chauhan (2013). Adhesins in human fungal pathogens: glue with plenty of stick. Eukaryot Cell 12, 470-81.
21. K.M. de Oliveira, B.R. da Silva Neto, J.A. Parente, R.A. da Silva, G.O. Quintino, A.R. Voltan, M.J. Mendes-Giannini, C.M. de Almeida Soares, and M. Pereira (2013). Intermolecular interactions of the malate synthase of Paracoccidioides spp. BMC Microbiol 13, 107.
22. L. de Pádua Queiroz, M.E. Mattos, M.F. da Silva, and C.L. Silva (2010). TGF-beta and CD23 are involved in nitric oxide production by pulmonary macrophages activated by beta-glucan from Paracoccidioides brasiliensis. Med Microbiol Immunol 199, 61-9.
23. A. Del Vecchio, J.e.F. Silva, J.L. Silva, P.F. Andreotti, C.P. Soares, G. Benard, and M.J. Giannini (2009). Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples. Mem Inst Oswaldo Cruz 104, 749-54.
24. M.L. Delgado, M.L. Gil, and D. Gozalbo (2003). Candida albicans TDH3 gene promotes secretion of internal invertase when expressed in Saccharomyces cerevisiae as a glyceraldehyde-3-phosphate dehydrogenase-invertase fusion protein. Yeast 20, 713-22.
25. F.C. Donofrio, A.C. Calil, E.T. Miranda, A.M. Almeida, G. Benard, C.P. Soares, S.N. Veloso, C.M. Soares, and M.J. Mendes Giannini (2009). Enolase from Paracoccidioides brasiliensis: isolation and identification as a fibronectin-binding protein. J Med Microbiol 58, 706-13.
26. M.S. Felipe, R.V. Andrade, F.B. Arraes, A.M. Nicola, A.Q. Maranhão, F.A. Torres, I. Silva-Pereira, M.J. Poças-Fonseca, E.G. Campos, L.M. Moraes, P.A. Andrade, A.H. Tavares, S.S. Silva, C.M. Kyaw, D.P. Souza, M. Pereira, R.S. Jesuíno, E.V. Andrade, J.A. Parente, G.S. Oliveira, M.S. Barbosa, N.F. Martins, A.L. Fachin, R.S. Cardoso, G.A. Passos, N.F. Almeida, M.E. Walter, C.M. Soares, M.J. Carvalho, M.M. Brígido, and P. Network (2005a). Transcriptional profiles of the human pathogenic fungus Paracoccidioides brasiliensis in mycelium and yeast cells. J Biol Chem 280, 24706-14.
27. M.S. Felipe, F.A. Torres, A.Q. Maranhão, I. Silva-Pereira, M.J. Poças-Fonseca, E.G. Campos, L.M. Moraes, F.B. Arraes, M.J. Carvalho, R.V. Andrade, A.M. Nicola, M.M. Teixeira, R.S. Jesuíno, M. Pereira, C.M. Soares, and M.M. Brígido (2005b). Functional genome of the human pathogenic fungus Paracoccidioides brasiliensis. FEMS Immunol Med Microbiol 45, 369-81.
28. C. Formosa, M. Schiavone, A. Boisrame, M.L. Richard, R.E. Duval, and E. Dague (2014). Multiparametric imaging of adhesive nanodomains at the surface of Candida albicans by atomic force microscopy. Nanomedicine.
29. M. Franco, M.T. Peracoli, A. Soares, R. Montenegro, R.P. Mendes, and D.A. Meira (1993). Host-parasite relationship in paracoccidioidomycosis. Curr Top Med Mycol 5, 115-49.
30. B.B. Fuchs, and E. Mylonakis (2006). Using non-mammalian hosts to study fungal virulence and host defense. Curr Opin Microbiol 9, 346-51.
31. G. Gegembauer, L.M. Araujo, E.F. Pereira, A.M. Rodrigues, A.M. Paniago, R.C. Hahn, and Z.P. de Camargo (2014). Serology of paracoccidioidomycosis due to Paracoccidioides lutzii. PLoS Negl Trop Dis 8, e2986.
32. S.A. Hanna, J.L. Monteiro da Silva, and M.J. Giannini (2000). Adherence and intracellular parasitism of Paracoccidioides brasiliensis in Vero cells. Microbes Infect 2, 877-84.
33. C.Y. Hung, J.J. Yu, K.R. Seshan, U. Reichard, and G.T. Cole (2002). A parasitic phase-specific adhesin of Coccidioides immitis contributes to the virulence of this respiratory Fungal pathogen. Infect Immun 70, 3443-56.
34. I.D. Jacobsen (2014). Galleria mellonella as a model host to study virulence of Candida. Virulence 5, 237-9.
35. P. Jiravanichpaisal, B.L. Lee, and K. Söderhäll (2006). Cell-mediated immunity in arthropods: hematopoiesis, coagulation, melanization and opsonization. Immunobiology 211, 213-36.
36. S.K. Lau, H. Tse, J.S. Chan, A.C. Zhou, S.O. Curreem, C.C. Lau, K.Y. Yuen, and P.C. Woo (2013). Proteome profiling of the dimorphic fungus Penicillium marneffei extracellular proteins and identification of glyceraldehyde-3-phosphate dehydrogenase as an important adhesion factor for conidial attachment. FEBS J 280, 6613-26.
37. P.e.S. Lima, L. Casaletti, A.M. Bailão, A.T. de Vasconcelos, G.a.R. Fernandes, and C.M. Soares (2014). Transcriptional and proteomic responses to carbon starvation in Paracoccidioides. PLoS Negl Trop Dis 8, e2855.
38. G.C. Machado (2011). "Espécies crípticas em Paracoccidioides brasiliensis: Diferenciação antigênica e de resposta a fatores estressantes " in Departament of Microbiology and Immunology, (Botucatu, São Paulo, Brazil: Univ Estadual Paulista), 70.
39. C.M. Marcos, J. de Fátima da Silva, H.C. de Oliveira, R.A. Moraes da Silva, M.J. Mendes-Giannini, and A.M. Fusco-Almeida (2012). Surface-expressed enolase contributes to the adhesion of Paracoccidioides brasiliensis to host cells. FEMS Yeast Res 12, 557-70.
40. C.M. Marcos, H.C. de Oliveira, J.e.F. da Silva, P.A. Assato, A.M. Fusco-Almeida, and M.J. Mendes-Giannini (2014). The multifaceted roles of metabolic enzymes in the Paracoccidioides species complex. Front Microbiol 5, 719.
41. D.R. Matute, J.G. McEwen, R. Puccia, B.A. Montes, G. San-Blas, E. Bagagli, J.T. Rauscher, A. Restrepo, F. Morais, G. Niño-Vega, and J.W. Taylor (2006). Cryptic speciation and recombination in the fungus Paracoccidioides brasiliensis as revealed by gene genealogies. Mol Biol Evol 23, 65-73.
42. M.J. Mendes-Giannini, P.F. Andreotti, L.R. Vincenzi, J.L. da Silva, H.L. Lenzi, G. Benard, R. Zancopé-Oliveira, H.L. de Matos Guedes, and C.P. Soares (2006). Binding of extracellular matrix proteins to Paracoccidioides brasiliensis. Microbes Infect 8, 1550-9.
43. M.J. Mendes-Giannini, S.A. Hanna, J.L. da Silva, P.F. Andreotti, L.R. Vincenzi, G. Benard, H.L. Lenzi, and C.P. Soares (2004). Invasion of epithelial mammalian cells by Paracoccidioides brasiliensis leads to cytoskeletal rearrangement and apoptosis of the host cell. Microbes Infect 6, 882-91.
44. M.J. Mendes-Giannini, J.L. Monteiro da Silva, J. de Fátima da Silva, F.C. Donofrio, E.T. Miranda, P.F. Andreotti, and C.P. Soares (2008). Interactions of Paracoccidioides brasiliensis with host cells: recent advances. Mycopathologia 165, 237-48.
45. M.J. Mendes-Giannini, L.C. Ricci, M.A. Uemura, E. Toscano, and C.W. Arns (1994). Infection and apparent invasion of Vero cells by Paracoccidioides brasiliensis. J Med Vet Mycol 32, 189-97.
46. M.J. Mendes-Giannini, M.L. Taylor, J.B. Bouchara, E. Burger, V.L. Calich, E.D. Escalante, S.A. Hanna, H.L. Lenzi, M.P. Machado, M. Miyaji, J.L. Monteiro Da Silva, E.M. Mota, A. Restrepo, S. Restrepo, G. Tronchin, L.R. Vincenzi, C.F. Xidieh, and E. Zenteno (2000). Pathogenesis II: fungal responses to host responses: interaction of host cells with fungi. Med Mycol 38 Suppl 1, 113-23.
47. T. Meshulam, S.M. Levitz, L. Christin, and R.D. Diamond (1995). A simplified new assay for assessment of fungal cell damage with the tetrazolium dye, (2,3)-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-tetrazolium-5-carboxanil ide (XTT). J Infect Dis 172, 1153-6.
48. C. Murciano, D.L. Moyes, M. Runglall, P. Tobouti, A. Islam, L.L. Hoyer, and J.R. Naglik (2012). Evaluation of the role of Candida albicans agglutinin-like sequence (Als) proteins in human oral epithelial cell interactions. PLoS One 7, e33362.
49. C.J. Nobile, H.A. Schneider, J.E. Nett, D.C. Sheppard, S.G. Filler, D.R. Andes, and A.P. Mitchell (2008). Complementary adhesin function in C. albicans biofilm formation. Curr Biol 18, 1017-24.
50. S.V. Nogueira, F.L. Fonseca, M.L. Rodrigues, V. Mundodi, E.A. Abi-Chacra, M.S. Winters, J.F. Alderete, and C.M. de Almeida Soares (2010). Paracoccidioides brasiliensis enolase is a surface protein that binds plasminogen and mediates interaction of yeast forms with host cells. Infect Immun 78, 4040-50.
51. A.F. Parente, A.M. Bailão, C.L. Borges, J.A. Parente, A.D. Magalhães, C.A. Ricart, and C.M. Soares (2011). Proteomic analysis reveals that iron availability alters the metabolic status of the pathogenic fungus Paracoccidioides brasiliensis. PLoS One 6, e22810.
52. L.A. Pereira, S.N. Báo, M.S. Barbosa, J.L. da Silva, M.S. Felipe, J.M. de Santana, M.J. Mendes-Giannini, and C.M. de Almeida Soares (2007). Analysis of the Paracoccidioides brasiliensis triosephosphate isomerase suggests the potential for adhesin function. FEMS Yeast Res 7, 1381-8.
53. R. Peres da Silva, M.T. Matsumoto, J.D. Braz, A.R. Voltan, H.C. de Oliveira, C.P. Soares, and M.J. Mendes Giannini (2011). Differential gene expression analysis of Paracoccidioides brasiliensis during keratinocyte infection. J Med Microbiol 60, 269-80.
54. N.S. Pitangui, J.C. Sardi, J.F. Silva, T. Benaducci, R.A. Moraes da Silva, G. Rodríguez-Arellanes, M.L. Taylor, M.J. Mendes-Giannini, and A.M. Fusco-Almeida (2012). Adhesion of Histoplasma capsulatum to pneumocytes and biofilm formation on an abiotic surface. Biofouling 28, 711-8.
55. R. Puccia, and L.R. Travassos (1991). 43-kilodalton glycoprotein from Paracoccidioides brasiliensis: immunochemical reactions with sera from patients with paracoccidioidomycosis, histoplasmosis, or Jorge Lobo's disease. J Clin Microbiol 29, 1610-5.
56. R. Puccia, M.C. Vallejo, A.L. Matsuo, and L.V. Longo (2011). The paracoccidioides cell wall: past and present layers toward understanding interaction with the host. Front Microbiol 2, 257.
57. S. Puri, W.K. Lai, J.M. Rizzo, M.J. Buck, and M. Edgerton (2014). Iron-responsive chromatin remodelling and MAPK signalling enhance adhesion in Candida albicans. Mol Microbiol 93, 291-305.
58. N. Ramarao, C. Nielsen-Leroux, and D. Lereclus (2012). The insect Galleria mellonella as a powerful infection model to investigate bacterial pathogenesis. J Vis Exp, e4392.
59. A.A. Rocha, F.V. Morais, and R. Puccia (2009). Polymorphism in the flanking regions of the PbGP43 gene from the human pathogen Paracoccidioides brasiliensis: search for protein binding sequences and poly(A) cleavage sites. BMC Microbiol 9, 277.
60. E. Ruiz-Baca, C. Toriello, A. Perez-Torres, M. Sabanero-Lopez, J.C. Villagomez-Castro, and E. Lopez-Romero (2009). Isolation and some properties of a glycoprotein of 70 kDa (Gp70) from the cell wall of Sporothrix schenckii involved in fungal adherence to dermal extracellular matrix. Med Mycol 47, 185-96.
61. G. Sandoval-Bernal, G. Barbosa-Sabanero, M. Shibayama, A. Perez-Torres, V. Tsutsumi, and M. Sabanero (2011). Cell wall glycoproteins participate in the adhesion of Sporothrix schenckii to epithelial cells. Mycopathologia 171, 251-9.
62. R.C. Silva, A.C. Padovan, D.C. Pimenta, R.C. Ferreira, C.V. da Silva, and M.R. Briones (2014). Extracellular enolase of Candida albicans is involved in colonization of mammalian intestinal epithelium. Front Cell Infect Microbiol 4, 66.
63. L.M. Singer-Vermes, E. Burger, M.F. Franco, M.M. Di-Bacchi, M.J. Mendes-Giannini, and V.L. Calich (1989). Evaluation of the pathogenicity and immunogenicity of seven Paracoccidioides brasiliensis isolates in susceptible inbred mice. J Med Vet Mycol 27, 71-82.
64. Y. Srinoulprasert, P. Kongtawelert, and S.C. Chaiyaroj (2006). Chondroitin sulfate B and heparin mediate adhesion of Penicillium marneffei conidia to host extracellular matrices. Microb Pathog 40, 126-32.
65. Y. Srinoulprasert, P. Pongtanalert, R. Chawengkirttikul, and S.C. Chaiyaroj (2009). Engagement of Penicillium marneffei conidia with multiple pattern recognition receptors on human monocytes. Microbiol Immunol 53, 162-72.
66. R.O. Suárez-Alvarez, A. Pérez-Torres, and M.L. Taylor (2010). Adherence patterns of Histoplasma capsulatum yeasts to bat tissue sections. Mycopathologia 170, 79-87.
67. M.L. Taylor, E. Duarte-Escalante, A. Pérez, E. Zenteno, and C. Toriello (2004). Histoplasma capsulatum yeast cells attach and agglutinate human erythrocytes. Med Mycol 42, 287-92.
68. M.M. Teixeira, R.C. Theodoro, M.J. de Carvalho, L. Fernandes, H.C. Paes, R.C. Hahn, L. Mendoza, E. Bagagli, G. San-Blas, and M.S. Felipe (2009a). Phylogenetic analysis reveals a high level of speciation in the Paracoccidioides genus. Mol Phylogenet Evol 52, 273-83.
69. P.A. Teixeira, R.A. de Castro, R.C. Nascimento, G. Tronchin, A.P. Torres, M. Lazéra, S.R. de Almeida, J.P. Bouchara, C.V. Loureiro y Penha, and L.M. Lopes-Bezerra (2009b). Cell surface expression of adhesins for fibronectin correlates with virulence in Sporothrix schenckii. Microbiology 155, 3730-8.
70. L. Thomaz, R. García-Rodas, A.J. Guimarães, C.P. Taborda, O. Zaragoza, and J.D. Nosanchuk (2013). Galleria mellonella as a model host to study Paracoccidioides lutzii and Histoplasma capsulatum. Virulence 4, 139-46.
71. S.K. Upadhyay, L. Mahajan, S. Ramjee, Y. Singh, S.F. Basir, and T. Madan (2009). Identification and characterization of a laminin-binding protein of Aspergillus fumigatus: extracellular thaumatin domain protein (AfCalAp). J Med Microbiol 58, 714-22.
72. M.C. Vallejo, A.L. Matsuo, L. Ganiko, L.C. Medeiros, K. Miranda, L.S. Silva, E. Freymüller-Haapalainen, R. Sinigaglia-Coimbra, I.C. Almeida, and R. Puccia (2011). The pathogenic fungus Paracoccidioides brasiliensis exports extracellular vesicles containing highly immunogenic a-Galactosyl epitopes. Eukaryot Cell 10, 343-51.
73. M.C. Vallejo, E.S. Nakayasu, L.V. Longo, L. Ganiko, F.G. Lopes, A.L. Matsuo, I.C. Almeida, and R. Puccia (2012a). Lipidomic analysis of extracellular vesicles from the pathogenic phase of Paracoccidioides brasiliensis. PLoS One 7, e39463.
74. M.C. Vallejo, E.S. Nakayasu, A.L. Matsuo, T.J. Sobreira, L.V. Longo, L. Ganiko, I.C. Almeida, and R. Puccia (2012b). Vesicle and vesicle-free extracellular proteome of Paracoccidioides brasiliensis: comparative analysis with other pathogenic fungi. J Proteome Res 11, 1676-85.
75. P. Vilmos, and E. Kurucz (1998). Insect immunity: evolutionary roots of the mammalian innate immune system. Immunol Lett 62, 59-66.
76. X.Y. Xu, Y. Shi, P.P. Zhang, F. Zhang, Y.Y. Shen, X. Su, and B.L. Zhao (2012). E cadherin mediates adhesion and endocytosis of Aspergillus fumigatus blastospores in human epithelial cells. Chin Med J (Engl) 125, 617-21.
77. G. Zhao, S. Li, W. Zhao, K. He, H. Xi, W. Li, Q. Zhou, and Y. Wang (2012). Phage display against corneal epithelial cells produced bioactive peptides that inhibit Aspergillus adhesion to the corneas. PLoS One 7, e33578.