A mass spectrometry-based method for the assay of ceramide synthase substrate specificity

Analytical Biochemistry

Volumn 478, Issue , 2015, Pages 96-101

  Luttgeharm, Kyle D ^a   Cahoon, Edgar B ^a   Markham, Jennifer E ^a  

^a UNIVERSITY OF NEBRASKA LINCOLN   (United States)

Author keywords

Ceramide synthase; Enzyme assay; Mass spectrometry; Sphingolipids; Substrate specificity

Indexed keywords

CERAMIDES; ENZYMES; MAMMALS; SUBSTRATES; UNSATURATED FATTY ACIDS; YEAST;

BOVINE SERUM ALBUMINS; CERAMIDE SYNTHASE; ENZYME ASSAYS; LIPID CLASS; LONG CHAINS; NON-ENZYMATIC; SPHINGOLIPIDS; SUBSTRATE CONCENTRATIONS; SUBSTRATE SPECIFICITY; SYNTHESISED;

MASS SPECTROMETRY;

BOVINE SERUM ALBUMIN; CERAMIDE SYNTHASE; FUNGAL ENZYME; PHYTOSPHINGOSINE; SPHINGANINE; SYNTHETASE; UNCLASSIFIED DRUG; CERAMIDE; DIHYDROCERAMIDE DESATURASE; FATTY ACID; OXIDOREDUCTASE; SPHINGOLIPID;

ARTICLE; COMPLEX FORMATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SPECIFICITY; HYDROLYSIS; IN VITRO STUDY; MICROSOME; NONHUMAN; PRIORITY JOURNAL; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SACCHAROMYCES CEREVISIAE; TANDEM MASS SPECTROMETRY; ENZYMOLOGY; MASS SPECTROMETRY; METABOLISM; PROCEDURES;

BOVINAE;

CERAMIDES; ENZYME ASSAYS; FATTY ACIDS; MASS SPECTROMETRY; OXIDOREDUCTASES; SACCHAROMYCES CEREVISIAE; SPHINGOLIPIDS; SUBSTRATE SPECIFICITY;

EID: 84926632026     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2015.02.016     Document Type: Article

References (39)

1. H.E. Townley, K. McDonald, G.I. Jenkins, M.R. Knight, and C.J. Leaver Ceramides induce programmed cell death in Arabidopsis cells in a calcium-dependent manner Biol. Chem. 386 2005 161 166
2. Y.A. Hannun, and C. Luberto Ceramide in the eukaryotic stress response Trends Cell Biol. 10 2000 73 80
3. R.C. Dickson, E.E. Nagiec, M. Skrzypek, P. Tillman, G.B. Wells, and R.L. Lester Sphingolipids are potential heat stress signals in Saccharomyces J. Biol. Chem. 272 1997 30196 30200
4. H. Wang, J. Li, R.M. Bostock, and D.G. Gilchrist Apoptosis: a functional paradigm for programmed plant cell death induced by a host-selective phytotoxin and invoked during development Plant Cell 8 1996 375 391
5. K.P. Alden, S. Dhondt-Cordelier, K.L. McDonald, T.J. Reape, C.K.Y. Ng, P.F. McCabe, and C.J. Leaver Sphingolipid long chain base phosphates can regulate apoptotic-like programmed cell death in plants Biochem. Biophys. Res. Commun. 410 2011 574 580
6. M. Maceyka, S.G. Payne, S. Milstien, and S. Spiegel Sphingosine kinase, sphingosine-1-phosphate, and apoptosis Biochim. Biophys. Acta 1585 2002 193 201
7. O. Cuvillier, G. Pirianov, B. Kleuser, P.G. Vanek, O.A. Coso, S. Gutkind, and S. Spiegel Suppression of ceramide-mediated programmed cell death by sphingosine-1-phosphate Nature 381 1996 800 803
8. S.D. Kobayashi, and M.M. Nagiec Ceramide/long-chain base phosphate rheostat in Saccharomyces cerevisiae: regulation of ceramide synthesis by Elo3p and Cka2p Eukaryot. Cell 2 2003 284 294
9. S. Aronova, K. Wedaman, P.A. Aronov, K. Fontes, K. Ramos, B.D. Hammock, and T. Powers Regulation of ceramide biosynthesis by TOR complex 2 Cell Metab. 7 2008 148 158
10. D.K. Breslow, and J.S. Weissman Membranes in balance: mechanisms of sphingolipid homeostasis Mol. Cell 40 2010 267 279
11. J.E. Markham, D. Molino, L. Gissot, Y. Bellec, K. Hematy, J. Marion, K. Belcram, J.C. Palauqui, B. Satiat-Jeunemaitre, and J.D. Faure Sphingolipids containing very-long-chain fatty acids define a secretory pathway for specific polar plasma membrane protein targeting in Arabidopsis Plant Cell 23 2011 2362 2378
12. M. Ali, J. Fritsch, H. Zigdon, Y. Pewzner-Jung, S. Schutze, and A.H. Futerman Altering the sphingolipid acyl chain composition prevents LPS/GLN-mediated hepatic failure in mice by disrupting TNFR1 internalization Cell Death Dis. 4 2013 e929
13. D. Hartmann, M.-S. Wegner, R.A. Wanger, N. Ferreirós, Y. Schreiber, J. Lucks, S. Schiffmann, G. Geisslinger, and S. Grösch The equilibrium between long and very long chain ceramides is important for the fate of the cell and can be influenced by co-expression of CerS Int. J. Biochem. Cell Biol. 45 2013 1195 1203
14. M. Chen, J.E. Markham, C.R. Dietrich, J.G. Jaworski, and E.B. Cahoon Sphingolipid long-chain base hydroxylation is important for growth and regulation of sphingolipid content and composition in Arabidopsis Plant Cell 20 2008 1862 1878
15. Y. Pewzner-Jung, S. Ben-Dor, and A.H. Futerman When do lasses (longevity assurance genes) become CerS (ceramide synthases)? Insights into the regulation of ceramide synthesis J. Biol. Chem. 281 2006 25001 25005
16. J.E. Markham, and J.G. Jaworski Rapid measurement of sphingolipids from Arabidopsis thaliana by reversed-phase high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry Rapid Commun. Mass Spectrom. 21 2007 1304 1314
17. M. Levy, and A.H. Futerman Mammalian ceramide synthases IUBMB Life 62 2010 347 356
18. E. Wang, and A.H. Merrill Jr. Ceramide synthase A.H. Merrill, Y.A. Hannun, Methods in Enzymology 2000 Academic Press San Diego 15 21
19. H. Shimeno, S. Soeda, M. Sakamoto, T. Kouchi, T. Kowakame, and T. Kihara Partial purification and characterization of sphingosine N-acyltransferase (ceramide synthase) from bovine liver mitochondrion-rich fraction Lipids 33 1998 601 605
20. B. Vallee, and H. Riezman Lip1p: a novel subunit of acyl-CoA ceramide synthase EMBO J. 24 2005 730 741
21. m values towards sphinganine FEBS Lett. 581 2007 5289 5294
22. K. Venkataraman, C. Riebeling, J. Bodennec, H. Riezman, J.C. Allegood, M.C. Sullards, A.H. Merrill Jr., and A.H. Futerman Upstream of growth and differentiation factor 1 (uog1), a mammalian homolog of the yeast longevity assurance gene 1 (LAG1), regulates N-stearoyl-sphinganine (C18-(dihydro)ceramide) synthesis in a fumonisin B1-independent manner in mammalian cells J. Biol. Chem. 277 2002 35642 35649
23. E.L. Laviad, L. Albee, I. Pankova-Kholmyansky, S. Epstein, H. Park, A.H. Merrill Jr., and A.H. Futerman Characterization of ceramide synthase 2: tissue distribution, substrate specificity, and inhibition by sphingosine 1-phosphate J. Biol. Chem. 283 2008 5677 5684
24. Y. Mizutani, A. Kihara, and Y. Igarashi LASS3 (longevity assurance homologue 3) is a mainly testis-specific (dihydro)ceramide synthase with relatively broad substrate specificity Biochem. J. 398 2006 531 538
25. C. Riebeling, J.C. Allegood, E. Wang, A.H. Merrill Jr., and A.H. Futerman Two mammalian longevity assurance gene (LAG1) family members, trh1 and trh4, regulate dihydroceramide synthesis using different fatty acyl-CoA donors J. Biol. Chem. 278 2003 43452 43459
26. Y. Mizutani, A. Kihara, and Y. Igarashi Mammalian Lass6 and its related family members regulate synthesis of specific ceramides Biochem. J. 390 2005 263 271
27. S. Narimatsu, S. Soeda, T. Tanaka, and Y. Kishimoto Solubilization and partial characterization of fatty acyl-CoA:sphingosine acyltransferase (ceramide synthetase) from rat liver and brain Biochim. Biophys. Acta 877 1986 334 341
28. H.J. Kim, Q. Qiao, H.D. Toop, J.C. Morris, and A.S. Don A fluorescent assay for ceramide synthase activity J. Lipid Res. 53 2012 1701 1707
29. R. Bauer, A. Voelzmann, B. Breiden, U. Schepers, H. Farwanah, I. Hahn, F. Eckardt, K. Sandhoff, and M. Hoch Schlank, a member of the ceramide synthase family controls growth and body fat in Drosophila EMBO J. 28 2009 3706 3716
30. E.V. Berdyshev, I. Gorshkova, A. Skobeleva, R. Bittman, X. Lu, S.M. Dudek, T. Mirzapoiazova, J.G.N. Garcia, and V. Natarajan FTY720 inhibits ceramide synthases and up-regulates dihydrosphingosine 1-phosphate formation in human lung endothelial cells J. Biol. Chem. 284 2009 5467 5477
31. S. Spassieva, J.G. Seo, J.C. Jiang, J. Bielawski, F. Varez-Vasquez, S.M. Jazwinski, Y.A. Hannun, and L.M. Obeid Necessary role for the Lag1p motif in (dihydro)ceramide synthase activity J. Biol. Chem. 281 2006 33931 33938
32. T.D. Mullen, S. Spassieva, R.W. Jenkins, K. Kitatani, J. Bielawski, Y.A. Hannun, and L.M. Obeid Selective knockdown of ceramide synthases reveals complex interregulation of sphingolipid metabolism J. Lipid Res. 52 2011 68 77
33. J.E. Markham, J. Li, E.B. Cahoon, and J.G. Jaworski Separation and identification of major plant sphingolipid classes from leaves J. Biol. Chem. 281 2006 22684 22694
34. K. Hirschberg, J. Rodger, and A.H. Futerman The long-chain sphingoid base of sphingolipids is acylated at the cytosolic surface of the endoplasmic reticulum in rat liver Biochem. J. 290 1993 751 757
35. M.-B. Mosbech, R. Kruse, E.B. Harvald, A.S.B. Olsen, S.F. Gallego, H.K. Hannibal-Bach, C.S. Ejsing, and N.J. Færgeman Functional loss of two ceramide synthases elicits autophagy-dependent lifespan extension in C. elegans PLoS One 8 2013 e70087
36. U. Acharya, and J.K. Acharya Enzymes of sphingolipid metabolism in Drosophila melanogaster Cell. Mol. Life Sci. 62 2005 128 142
37. M.D. Ullman, and N.S. Radin Enzymatic formation of hydroxy ceramides and comparison with enzymes forming nonhydroxy ceramides Arch. Biochem. Biophys. 152 1972 767 777
38. H. Van Overloop, G. Van der Hoeven, and P.P. Van Veldhoven N-Acyl migration in ceramides J. Lipid Res. 46 2005 812 816
39. E.W. Richards, M.W. Hamm, J.E. Fletcher, and D.A. Otto The binding of palmitoyl-CoA to bovine serum albumin Biochim. Biophys. Acta 1044 1990 361 367