Identification of mouse T follicular helper cells by flow cytometry

Methods in Molecular Biology

Volumn 1291, Issue , 2015, Pages 3-11

  Meli, Alexandre P ^a   King, Irah L ^a  

^a NONE

Author keywords

Antibody; Flow cytometry; Germinal center; Lymphocyte; Murine; Phenotype; Secondary lymphoid organ; T follicular helper cell

Indexed keywords

CD4 ANTIGEN; CHEMOKINE RECEPTOR CCR7; CHEMOKINE RECEPTOR CXCR4; CHEMOKINE RECEPTOR CXCR5; INTERLEUKIN 21; INTERLEUKIN 4; P SELECTIN GLYCOPROTEIN LIGAND 1;

ANIMAL CELL; ARTICLE; CD4+ T LYMPHOCYTE; CELL DIFFERENTIATION; CELL FUNCTION; EX VIVO STUDY; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; HUMORAL IMMUNITY; INFECTION; LEUKOCYTE; LYMPH NODE; MOUSE; NONHUMAN; PEYER PATCH; PRIORITY JOURNAL; SPLEEN; T FOLLICULAR HELPER CELL; ANIMAL; C57BL MOUSE; CELL SEPARATION; CYTOLOGY; HELPER CELL; PROCEDURES; STAINING;

MURINAE; MUS;

ANIMALS; CELL SEPARATION; FLOW CYTOMETRY; MICE, INBRED C57BL; STAINING AND LABELING; T-LYMPHOCYTES, HELPER-INDUCER;

EID: 84926623073     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2498-1_1     Document Type: Article

References (21)

1. Crotty S (2011) Follicular helper CD4 T cells (Tfh). Annu Rev Immunol 29:621–663
2. Victora DG, Nussenzweig MC (2012) Germinal centers. Annu Rev Immunol 30:429–457
3. Kaji T, Ishige A, Hikida M et al (2012) Distinct cellular pathways select germline-encoded and somatically mutated antibodies into immunological memory. J Exp Med 209: 2079–2097
4. Kato LM, Kawamoto S, Maruya M et al (2014) Gut TFH and IgA: key players for regulation of bacterial communities and immune homeostasis. Immunol Cell Biol 92:49–56
5. Breitfeld D, Ohl L, Kremmer E et al (2000) Follicular B helper T cells express CXC chemokine receptor 5, localize to B cell follicles, and support immunoglobulin production. J Exp Med 192:1545–1552
6. Schaerli P, Willimann K, Lang AB et al (2000) CXC chemokine receptor 5 expression defines follicular homing T cells with B cell helper function. J Exp Med 192:1553–1562
7. Nurieva RI, Chung Y, Martinez GJ et al (2009) Bcl6 mediates the development of T follicular helper cells. Science 325:1001–1005
8. Johnston RJ, Poholek AC, DiToro D et al (2009) Bcl6 and blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation. Science 325:1006–1010
9. Yu D, Rao S, Tsai LM et al (2009) The transcriptional repressor Bcl-6 directs T follicular helper cell lineage commitment. Immunity 31:457–468
10. Liu X, Chen X, Zhong B et al (2014) Transcription factor achaete-scute homologue 2 initiates follicular T-helper-cell development. Nature 507:513–518
11. Haynes NM, Allen CD, Lesley R et al (2007) Role of CXCR5 and CCR7 in follicular Th cell positioning and appearance of a programmed cell death gene-1 high germinal centerassociated subpopulation. J Immunol 179: 5099–6018
12. Akiba H, Takeda K, Kojima Y et al (2005) The role of ICOS in the CXCR5 + follicular B helper T cell maintenance in vivo. J Immunol 175: 2340–2348
13. Tangye SG, Ma CS, Brink R et al (2013) The good, the bad and the ugly—Tfh cells in human health and disease. Nat Rev Immunol 13:412–426
14. Poholek AC, Hansen K, Hernandez SG et al (2010) In vivo regulation of Bcl6 and T follicular helper cell development. J Immunol 185:313–326
15. Crotty S, Kersh EN, Cannons J et al (2003) SAP is required for generating long-term humoral immunity. Nature 421:282–287
16. Chtanova T, Tangye SG, Newton R et al (2004) T follicular helper cells express a distinctive transcriptional profile, reflecting their role as non-Th1/Th2 effector cells that provide help for B cells. J Immunol 173:68–78
17. King IL and Mohrs M (2009) IL-4 producing CD4+ T cells in reactive lymph nodes during helminth infection are T follicular helper cells. J Exp Med 206:1001–1007
18. Chung Y, Tanaka S, Chu F et al (2011) Follicular regulatory T cells expressing Foxp3 and Bcl-6 suppress germinal center reactions. Nat Med 17:983–988
19. Linterman MA, Pierson W, Lee SK et al (2011) Foxp3 + follicular regulatory T cells control the germinal center response. Nat Med 17: 975–982
20. He J, Tsai LM, Leong YA et al (2013) Circulating precursor CCR7(lo)PD-1(hi) CXCR5 + CD4 + T cells indicate Tfh cell activity and promote antibody responses upon antigen reexposure. Immunity 17:770–781
21. Fahey LM, Wilson EB, Elsaesser H et al (2011) Viral persistence redirects CD4 T cell differentiation toward T follicular helper cells. J Exp Med 208:987–999