Reactive Oxygen Species and Cytotoxicity in Rainbow Trout Hepatocytes: Effects of Medium and Incubation Time

Bulletin of Environmental Contamination and Toxicology

Volumn 94, Issue 2, 2015, Pages 193-198

  Yazdani, Mazyar ^a   Paulsen, Ragnhild Elisabeth ^a   Gjøen, Tor ^a   Hylland, Ketil ^a  

^a UNIVERSITY OF OSLO   (Norway)

Author keywords

Culture age; Fluorescent probes; Hepatocytes; Incubation time; Oncorhynchus mykiss; ROS

Indexed keywords

FLUORESCENCE; PROBES; OXYGEN;

FLUORESCENT PROBES; HEPATOCYTES; INCUBATION TIME; ONCORHYNCHUS MYKISS; PRIMARY CULTURE; PRIMARY HEPATOCYTE; REACTIVE OXYGEN SPECIES; TRIS-BUFFERED SALINES; ROS;

CYTOTOXICITY;

ONCORHYNCHUS MYKISS;

COPPER; CULTURE MEDIUM; POLLUTANT; REACTIVE OXYGEN METABOLITE;

ANIMAL; CELL CULTURE; CULTURE MEDIUM; DRUG EFFECTS; LIVER CELL; METABOLISM; ONCORHYNCHUS MYKISS; POLLUTANT; TOXICITY;

ANIMALS; CELLS, CULTURED; COPPER; CULTURE MEDIA; ENVIRONMENTAL POLLUTANTS; HEPATOCYTES; ONCORHYNCHUS MYKISS; REACTIVE OXYGEN SPECIES;

EID: 84926466982     PISSN: 00074861     EISSN: 14320800     Source Type: Journal    
DOI: 10.1007/s00128-014-1433-0     Document Type: Article

References (41)

1. Boess F, Kamber M, Romer S, Gasser R, Muller D, Albertini S, Suter L (2003) Gene expression in two hepatic cell lines, cultured primary hepatocytes, and liver slices compared to the in vivo liver gene expression in rats: possible implications for toxicogenomics use of in vitro systems. Toxicol Sci 73:386-402. doi: 10.1093/toxsci/kfg064
2. Bonney R (1974) Adult liver parenchymal cells in primary culture: characteristics and cell recognition standards. In Vitro 10:130-142. doi: 10.1007/bf02615346
3. Boone AN, Vijayan MM (2002) Constitutive heat shock protein 70 (HSC70) expression in rainbow trout hepatocytes: effect of heat shock and heavy metal exposure. Comp Biochem Physiol Part C Toxicol Pharmacol 132:223-233. doi: 10.1016/S1532-0456(02)00066-2
4. Bopp SK, Abicht HK, Knauer K (2008) Copper-induced oxidative stress in rainbow trout gill cells. Aquat Toxicol 86:197-204
5. +-ATPase. J Microbiol Methods 31:113-125. doi: 10.1016/s0167-7012(97)00095-x
6. Britton RS (1996) Metal-induced hepatotoxicity. Semin Liver Dis 16:3-12
7. Croci T, Wolliams GM (1985) Activities of several phase I and phase II xenobiotic biotransformation enzymes in cultured hepatocytes from male and female rats. Biochem Pharmacol 34:3029-3035
8. Ellesat KS, Yazdani M, Holth TF, Hylland K (2011) Species-dependent sensitivity to contaminants: an approach using primary hepatocyte cultures with three marine fish species. Mar Environ Res 72:216-224. doi: 10.1016/j.marenvres.2011.09.003
9. Farmen E, Olsvik PA, Berntssen MHG, Hylland K, Tollefsen KE (2010) Oxidative stress responses in rainbow trout (Oncorhynchus mykiss) hepatocytes exposed to pro-oxidants and a complex environmental sample. Comp Biochem Physiol Part C Toxicol Pharmacol 151:431-438. doi: 10.1016/j.cbpc.2010.01.008
10. Ferraris M, Radice S, Catalani P, Francolini M, Marabini L, Chiesara E (2002) Early oxidative damage in primary cultured trout hepatocytes: a time course study. Aquat Toxicol 59:283-296. doi: 10.1016/s0166-445x(02)00007-3
11. Gille JJP, Joenje H (1992) Cell culture models for oxidative stress: Superoxide and hydrogen peroxide versus normobaric hyperoxia. Mutat Res/DNAging 275:405-414
12. Gomes A, Fernandes E, Lima JLFC (2005) Fluorescence probes used for detection of reactive oxygen species. J Biochem Biophys Methods 65:45-80. doi: 10.1016/j.jbbm.2005.10.003
13. Grzelak A, Rychlik B, Bartosz G (2001) Light-dependent generation of reactive oxygen species in cell culture media. Free Radic Biol Med 30:1418-1425. doi: 10.1016/S0891-5849(01)00545-7
14. Halliwell B (2003) Oxidative stress in cell culture: an under-appreciated problem? FEBS Lett 540:3-6. doi: 10.1016/S0014-5793(03)00235-7
15. Halliwell B, Gutteridge J (2007) Free radicals in biology and medicine. Oxford University Press, Oxford
16. Hempel SL, Buettner GR, O'Malley YQ, Wessels DA, Flaherty DM (1999) Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2′,7′-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2′,7′-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free Radic Biol Med 27:146-159. doi: 10.1016/s0891-5849(99)00061-1
17. Heyne B, Beddie C, Scaiano JC (2007) Synthesis and characterization of a new fluorescent probe for reactive oxygen species. Org Biomol Chem 5:1454-1458
18. Jos A, Cameán AM, Pflugmacher S, Segner H (2009) The antioxidant glutathione in the fish cell lines EPC and BCF-2: response to model pro-oxidants as measured by three different fluorescent dyes. Toxicol In Vitro 23:546-553. doi: 10.1016/j.tiv.2009.01.013
19. Kooy NW, Royall JA, Ischiropoulos H, Beckman JS (1994) Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radic Biol Med 16:149-156. doi: 10.1016/0891-5849(94)90138-4
20. Krumschnabel G, Manzl C, Berger C, Hofer B (2005) Oxidative stress, mitochondrial permeability transition, and cell death in Cu-exposed trout hepatocytes. Toxicol Appl Pharmacol 209:62-73
21. Manzl C, Enrich J, Ebner H, Dallinger R, Krumschnabel G (2004) Copper-induced formation of reactive oxygen species causes cell death and disruption of calcium homeostasis in trout hepatocytes. Toxicology 196:57-64
22. Misra S, Niyogi S (2009) Selenite causes cytotoxicity in rainbow trout (Oncorhynchus mykiss) hepatocytes by inducing oxidative stress. Toxicol In Vitro 23:1249-1258. doi: 10.1016/j.tiv.2009.07.031
23. Nawaz M, Manzl C, Krumschnabel G (2005) Toxicity of copper, cadmium, and chromium to isolated hepatocytes from carp, Cyprinus carpio L. Bull Environ Contam Toxicol 75:652-661. doi: 10.1007/s00128-005-0802-0
24. Nishimoto M, Roubal WT, Stein JE, Varanasi U (1991) Oxidative DNA damage in tissues of English sole (Parophrys vetulus) exposed to nitrofurantoin. Chem-Biol Interact 80:317-326. doi: 10.1016/0009-2797(91)90091-K
25. Otto DME, Moon TW (1996) Phase I and II enzymes and antioxidant responses in different tissues of brown bullheads from relatively polluted and non-polluted systems. Arch Environ Contam Toxicol 31:141-147. doi: 10.1007/bf00203918
26. Pedrajas JR, Peinado J, López-Barea J (1995) Oxidative stress in fish exposed to model xenobiotics. Oxidatively modified forms of Cu, Zn-superoxide dismutase as potential biomarkers. Chem-Biol Interact 98:267-282. doi: 10.1016/0009-2797(95)03651-2
27. Pesonen M, Andersson TB (1997) Fish primary hepatocyte culture; an important model for xenobiotic metabolism and toxicity studies. Aquat Toxicol 37:253-267. doi: 10.1016/S0166-445X(96)00811-9
28. 2+. Toxicology 143:263-273
29. Radice S, Fumagalli R, Chiesara E, Ferraris M, Frigerio S, Marabini L (2004) Estrogenic activity of procymidone in rainbow trout (Oncorhynchus mykiss) hepatocytes: a possible mechanism of action. Chem-Biol Interact 147:185-193. doi: 10.1016/j.cbi.2003.12.006
30. Rau MA, Whitaker J, Freedman JH, Di Giulio RT (2004) Differential susceptibility of fish and rat liver cells to oxidative stress and cytotoxicity upon exposure to prooxidants. Comp Biochem Physiol Part C Toxicol Pharmacol 137:335-342
31. Rico D, Martín-González A, Díaz S, de Lucas P, Gutiérrez J-C (2009) Heavy metals generate reactive oxygen species in terrestrial and aquatic ciliated protozoa. Comp Biochem Physiol Part C Toxicol Pharmacol 149:90-96. doi: 10.1016/j.cbpc.2008.07.016
32. Roy DN, Mandal S, Sen G, Biswas T (2009) Superoxide anion mediated mitochondrial dysfunction leads to hepatocyte apoptosis preferentially in the periportal region during copper toxicity in rats. Chem-Biol Interact 182:136-147. doi: 10.1016/j.cbi.2009.08.014
33. Ruch RJ, Crist KA, Klaunig JE (1989) Effects of culture duration on hydrogen peroxide-induced hepatocyte toxicity. Toxicol Appl Pharmacol 100:451-464. doi: 10.1016/0041-008X(89)90293-7
34. Schreer A, Tinson C, Sherry JP, Schirmer K (2005) Application of Alamar blue/5-carboxyfluorescein diacetate acetoxymethyl ester as a noninvasive cell viability assay in primary hepatocytes from rainbow trout. Anal Biochem 344:76-85. doi: 10.1016/j.ab.2005.06.009
35. Sorg O (2004) Oxidative stress: a theoretical model or a biological reality? Comptes Rendus Biol 327:649-662
36. Tarpey MM, Wink DA, Grisham MB (2004) Methods for detection of reactive metabolites of oxygen and nitrogen: in vitro and in vivo considerations. Am J Physiol Regul Integr Comp Physiol 286:R431-R444. doi: 10.1152/ajpregu.00361.2003
37. Tollefsen KE, Mathisen R, Stenersen J (2003) Induction of vitellogenin synthesis in an Atlantic salmon (Salmo salar) hepatocyte culture: a sensitive in vitro bioassay for the oestrogenic and anti-oestrogenic activity of chemicals. Biomarkers 8:394-407
38. Weglarz L, Bartosz G (1991) Hydralizine stimulates production of oxygen free radicals in eagle's medium and cultured fibroblasts. Free Radic Biol Med 11:149-155. doi: 10.1016/0891-5849(91)90165-Y
39. Winston GW, Di Giulio RT (1991) Prooxidant and antioxidant mechanisms in aquatic organisms. Aquat Toxicol 19:137-161. doi: 10.1016/0166-445x(91)90033-6
40. Wright J, George S, Martinez-Lara E, Carpene E, Kindt M (2000) Levels of cellular glutathione and metallothionein affect the toxicity of oxidative stressors in an established carp cell line. Mar Environ Res 50:503-508. doi: 10.1016/s0141-1136(00)00125-2
41. Zar JH (2010) Biostatistical analysis, 5th edn. Prentice-Hall/Pearson, Saddle River