Immunocapture and microplate-based activity and quantity measurement of pyruvate dehydrogenase in human peripheral blood mononuclear cells

Bioanalysis

Volumn 7, Issue 5, 2015, Pages 583-592

  Liu, Xiaowen ^a   Pervez, Hira ^a   Andersen, Lars W ^a,b   Uber, Amy ^a   Montissol, Sophia ^a   Patel, Parth ^a   Donnino, Michael W ^a  

^a BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

^b AARHUS UNIVERSITY HOSPITAL   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

DETERGENT; PROTEINASE INHIBITOR; PYRUVATE DEHYDROGENASE; PYRUVATE DEHYDROGENASE COMPLEX;

ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; FREEZE THAWING; HUMAN; HUMAN CELL; IMMUNOASSAY; IMMUNOCAPTURE; MALE; MICROPLATE ASSAY; MITOCHONDRIAL MEMBRANE; MOUSE; NONHUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PROTEIN CONTENT; PROTEIN PROCESSING; QUANTITATIVE ANALYSIS; IMMUNOLOGY; MONONUCLEAR CELL;

HUMANS; LEUKOCYTES, MONONUCLEAR; PYRUVATE DEHYDROGENASE COMPLEX;

EID: 84926386626     PISSN: 17576180     EISSN: 17576199     Source Type: Journal    
DOI: 10.4155/bio.14.302     Document Type: Article

References (21)

1. Garcia-Alvarez M, Marik P, Bellomo R. Sepsis-associated hyperlactatemia. Crit. Care 18(5), 503-514 (2014).
2. Cross JH, Connelly A, Gadian DG et al. Clinical diversity of pyruvate dehydrogenase deficiency. Pediatr. Neurol. 10(4), 276-283 (1994).
3. Donnino M. Gastrointestinal beriberi: a previously unrecognized syndrome. Ann. Intern. Med. 141(11), 898-899 (2004).
4. Kuriyama M, Yokomine R, Arima H, Hamada R, Igata A. Blood vitamin B1, transketolase and thiamine pyrophosphate (TPP) effect in beriberi patients, with studies employing discriminant analysis. Clin. Chim. Acta 108(2), 159-168 (1980).
5. Donnino MW, Vega J, Miller J, Walsh M. Myths and misconceptions of Wernicke's encephalopathy: what every emergency physician should know. Ann. Emerg. Med. 50(6), 715-721 (2007).
6. Page GL, Laight D, Cummings MH. Thiamine deficiency in diabetes mellitus and the impact of thiamine replacement on glucose metabolism and vascular disease. Int. J. Clin. Pract. 65(6), 684-690 (2011).
7. Donnino MW, Carney E, Cocchi MN et al. Thiamine deficiency in critically ill patients with sepsis. J. Crit. Care. 25(4), 576-581 (2010).
8. Donnino MW, Cocchi MN, Smithline H, Carney E, Chou PP, Salciccioli J. Coronary artery bypass graft surgery depletes plasma thiamine levels. Nutrition 26(1), 133-136 (2010).
9. Moskowitz A, Graver A, Giberson T et al. The relationship between lactate and thiamine levels in patients with diabetic ketoacidosis. J. Crit. Care. 29(2), 6 (2013).
10. Bonnet S, Archer SL, Allalunis-Turner J et al. A mitochondria-K+ channel axis is suppressed in cancer and its normalization promotes apoptosis and inhibits cancer growth. Cancer Cell 11(1), 37-51 (2007).
11. McMurtry MS, Bonnet S, Wu X et al. Dichloroacetate prevents and reverses pulmonary hypertension by inducing pulmonary artery smooth muscle cell apoptosis. Circ. Res. 95(8), 830-840 (2004).
12. Michelakis ED, McMurtry MS, Wu XC et al. Dichloroacetate, a metabolic modulator, prevents and reverses chronic hypoxic pulmonary hypertension in rats: role of increased expression and activity of voltage-gated potassium channels. Circulation 105(2), 244-250 (2002).
13. Patel MS, Korotchkina LG. Regulation of the pyruvate dehydrogenase complex. Biochem. Soc. Trans. 34(Pt 2), 217-222 (2006).
14. Linn TC, Pettit FH, Reed LJ. Alpha-keto acid dehydrogenase complexes. X. Regulation of the activity of the pyruvate dehydrogenase complex from beef kidney mitochondria by phosphorylation and dephosphorylation. Proc. Natl Acad. Sci. USA 62(1), 234-241 (1969).
15. Haas RH, Thompson G, Morris B, Conright K, Andrews T. Pyruvate dehydrogenase activity in osmotically shocked rat brain mitochondria: stimulation by oxaloacetate. J. Neurochem. 50(3), 673-680 (1988).
16. Ksiezak-Reding H, Blass JP, Gibson GE. Studies on the pyruvate dehydrogenase complex in brain with the arylamine acetyltransferase-coupled assay. J. Neurochem. 38(6), 1627-1636 (1982).
17. Schofield PJ, Griffiths LR, Rogers SH, Wise G. An improved method for the assay of platelet pyruvate dehydrogenase. Clin. Chim. Acta 108(2), 219-227 (1980).
18. Lib M, Rodriguez-Mari A, Marusich MF, Capaldi RA. Immunocapture and microplate-based activity measurement of mammalian pyruvate dehydrogenase complex. Anal. Biochem. 314(1), 121-127 (2003).
19. Atherton HJ, Schroeder MA, Dodd MS et al. Validation of the in vivo assessment of pyruvate dehydrogenase activity using hyperpolarised 13C MRS. NMR Biomed. 24(2), 201-208 (2011).
20. Levy MM, Fink MP, Marshall JC et al. 2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference. Crit. Care Med. 31(4), 1250-1256 (2003).
21. Brooks SP, Storey KB. An improvement in the pyruvate dehydrogenase complex assay: a high-yield method for purifying arylamine acetyltransferase. Anal. Biochem. 212(2), 452-456 (1993).