Tandem-yeast expression system for engineering and producing unspecific peroxygenase

Enzyme and Microbial Technology

Volumn 73-74, Issue , 2015, Pages 29-33

  Molina Espeja, Patricia ^a   Ma, Su ^b   Mate, Diana M ^c   Ludwig, Roland ^b   Alcalde, Miguel ^a  

^a INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

^b UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^c DWI LEIBNIZ INSTITUTE FOR INTERACTIVE MATERIALS   (Germany)

Author keywords

Directed evolution; Functional expression; Pichia pastoris; Saccharomyces cerevisiae; Unspecific peroxygenase (UPO)

Indexed keywords

CATALYST ACTIVITY; FERMENTATION; PHYSIOLOGY;

ALCOHOL OXIDASE 1 PROMOTER; BIOTECHNOLOGICAL APPLICATIONS; DIRECTED EVOLUTION; FUNCTIONAL EXPRESSION; HETEROLOGOUS EXPRESSION; MONOOXYGENASE ACTIVITIES; PICHIA PASTORIS; UNSPECIFIC PEROXYGENASE (UPO);

YEAST;

ALCOHOL OXIDASE; METHANOL; OXYGENASE; UNCLASSIFIED DRUG; UNSPECIFIC PEROXYGENASE; ALCOHOL DEHYDROGENASE; FUNGAL PROTEIN; HYBRID PROTEIN; MIXED FUNCTION OXIDASE; PEROXYGENASE;

ARTICLE; CELL DENSITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME GLYCOSYLATION; ENZYME KINETICS; ENZYME RELEASE; ENZYME STABILITY; ENZYME SYNTHESIS; FED BATCH FERMENTATION; FUNGUS MUTATION; GENE EXPRESSION SYSTEM; KOMAGATAELLA PASTORIS; LARGE SCALE PRODUCTION; MOLECULAR WEIGHT; NONHUMAN; OXIDATION REDUCTION POTENTIAL; PH; PROMOTER REGION; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; TANDEM YEAST EXPRESSION SYSTEM; YEAST; AGROCYBE; BIOSYNTHESIS; DIRECTED MOLECULAR EVOLUTION; ENZYMOLOGY; FERMENTATION; FUNGAL GENE; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PICHIA; PROCEDURES; PROTEIN ENGINEERING;

AGROCYBE AEGERITA; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

AGROCYBE; ALCOHOL OXIDOREDUCTASES; DIRECTED MOLECULAR EVOLUTION; FERMENTATION; FUNGAL PROTEINS; GENES, FUNGAL; MIXED FUNCTION OXYGENASES; PICHIA; PROMOTER REGIONS, GENETIC; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE;

EID: 84926148616     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2015.03.004     Document Type: Article

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