Isothermal titration calorimetry for studying protein-ligand interactions

Methods in Molecular Biology

Volumn 1008, Issue , 2013, Pages 103-118

  Damian, Luminita ^a  

^a GE HEALTHCARE   (United Kingdom)

Author keywords

Affinity constant; Binding constant; Competition experiments; Dissociation constant; Drug discovery; Enthalpy change; Entropy change; Equilibrium constant; Gibbs free energy change; Heat capacity change; Hydrogen bond; Hydrophobic interactions; Isothermal titration calorimetry; ITC; Lead discovery; Lead optimization; Ligand binding; Protein interactions; Stoichiometry; Thermodynamics

Indexed keywords

LIGAND; PROTEIN; PROTEIN BINDING;

ARTICLE; CONTROLLED STUDY; ENTROPY; ISOTHERMAL TITRATION CALORIMETRY; LIGAND BINDING; PRIORITY JOURNAL; PROTEIN INTERACTION; STOICHIOMETRY; THERMODYNAMICS; BINDING COMPETITION; CALORIMETRY; CHEMICAL PHENOMENA; CHEMISTRY; DRUG DEVELOPMENT; HUMAN; HYDROGEN BOND; KINETICS; PROCEDURES; STEREOISOMERISM;

BINDING, COMPETITIVE; CALORIMETRY; DRUG DISCOVERY; HUMANS; HYDROGEN BONDING; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; KINETICS; LIGANDS; PROTEIN BINDING; PROTEINS; STEREOISOMERISM; THERMODYNAMICS;

EID: 84925559402     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-398-5_4     Document Type: Article

References (17)

1. Ladbury JE, Klebe G, Freire E (2010) Adding calorimetric data to decision making in lead discovery: A hot tip. Nat Rev Drug Discov 9:23-27
2. Atkins P, de Paula J (2002) Physical chemistry, 7th edn.OxfordUniversity Press Inc.,NewYork
3. Holdgate GA, Ward WHJ (2005) Measurements of binding thermodynamics in drug discovery. Drug Discov Today 10:1543-1550
4. Wiseman T et al (1989) Rapid measurement of binding constants and heats of binding using a new titration calorimeter. Anal Biochem 179:131-137
5. Ward WHJ, Holdgate GA (2001) Isothermal titration calorimetry in drug discovery. Prog Med Chem 38:309-376
6. Sturtevant JM (1977) Heat capacity and entropy changes in processes involving proteins. Proc Natl Acad Sci U S A 74:2236-2240
7. Niedzwiecka A et al (2002) Positive heat capacity change upon specific binding of translation initiation factor eIF4E to mRNA 5cap. Biochemistry 41:12140-12148
8. Christensen JJ, Hansen LD, Izatt RM (1976) Handbook of proton ionization heats and related thermodynamic quantities. Wiley, New York
9. Fukada H, Takahashi K (1998) Enthalpy and heat capacity changes for the proton dissociation of various buffer components in 0.1 M potassium chloride. Proteins 33:159-166
10. Leavitt S, FreireE(2001)Directmeasurement of protein binding energetics by isothermal titration calorimetry. Curr Opin Struct Biol 11:560-566
11. Jelesarov I, Bosshard HR (1999) Isothermal titration calorimetry and differential scanning calorimetry as complementary tools to investigate the energetics of biomolecular recognition. J Mol Recognit 12:3-18
12. Garidel P et al Understanding the self organisation of Association Colloids: Microcal Application Note. http://www.microcal. com/documents/ colloidsappnote.pdf
13. Todd MJ, Gomez J (2001) Enzyme kinetics determined using calorimetry: A general assay for enzyme activity? Anal Biochem 296:179-187
14. Myszka DG et al (2003) The ABRF-MIRG02 study: Assembly state, thermodynamic and kinetic analysis of an enzyme/inhibitor interaction. J Biomol Tech 14:247-269
15. OBrien R et al (1998) The effects of salt on the TATA binding protein-DNA interaction from a hyperthermophilic archaeon. J Mol Biol 279:117-125
16. Turnbull WB, Daranas AH (2003) On the value of c: Can low affinity systems be studied by isothermal titration calorimetry? J Am Chem Soc 125:14859-14866
17. Velazquez-Campoy A, Freire E (2006) Isothermal titration calorimetry to determine association constants for high-Affinity ligands. Nat Protoc 1:186-191